Diagnosis of potato virus Y<sup>N</sup>: a comparison between polyclonal and monoclonal antibodies and a biological assay

D, Rose; McCARRA, Susan; Mitchell, D. H.

doi:10.1111/j.1365-3059.1987.tb02182.x

Cited by 12 publications

(7 citation statements)

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“…The introduction of strain-specific MAbs represents a significant advancement in the detection and diagnosis of the various PVY strains [15,16,52]. PVY C and PVY O are serologically distinguishable, and it is possible to distinguish these using monoclonal antibodies that recognise a specific epitopes [42,58].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of potato virus Y isolates from Iran

et al. 2010

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A survey of Potato virus Y (PVY) was conducted in cultivated fields in six Iranian provinces between January 2005 to July 2007. Two hundred samples from potato and tomato were collected and analyzed by enzyme-linked immunosorbent assay (ELISA) for potyviruses. Almost one fourth of the samples were found to be infected by PVY. Analysis of these PVY-positive samples using three monoclonal antibodies (MAbs) facilitating the simultaneous detection of three main strains namely the ordinary (PVY(O)), strain (PVY(N)) and C (PVY(C)) strains. However, the fourth strain (PVY(NTN)) and some others recombinant isolates were also identified by molecular methods. Host range and symptoms analysis using sap inoculation of four different strains of PVY onto a range of plants revealed that the four strains showed biological properties that seemed to be consistent with their molecular grouping. Fourteen isolates of PVY were chosen based on the host and geographical location, primer specificity and serology for further biological and molecular characterisation. The coat protein (CP) and P1 genes and 3'-non-translated region (3'NTR) from 14 representative isolates were sequenced and analysed with the sequences available in GenBank. Composite analysis of the P1, CP and 3'-UTR sequences with all full genome sequences of PVY revealed that there are three potential strains of PVY in Iran, PVY(O), PVY(N)-W and PVY(NTN). Isolate KER.SA(N) was the most divergent of all the 14 isolates reacted with PVY(N) specific MAbs but grouped with PVY(O) strains in maximum likelihood phylogentic analysis. The PVY(NTN) isolates from Iran more closely related to the European than North American PVY(NTN) isolates.

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Section: Introductionmentioning

confidence: 99%

Characterisation of potato virus Y isolates from Iran

et al. 2010

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“…There are many instances of monoclonal antibodies being used to discriminate between strains of virus, bacteria or fungi. For example, there are monoclonal antibodies that will differentiate between the veinal necrosis and wild-type strains of potato virus Y (Rose, McCarra and Mitchell, 1987), between pathovars of Xanthomonas campestris (Alvarez, Benedict and Mizumoto, 1985) and between Sirococcus strobilinus strains that colonize western hemlock (Tsuga heterophylla) and those that colonize either pine (Pinus sp.) or spruce (Picea sp.)…”

Section: Serological Methodsmentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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“…PVY strains are known to be closely related serologically and no consistent serological differences have been observed between the three main strain groups using polyclonal antisera [5]. However, some monoclonal antibodies have been produced which only recognize strains in the PVY N group and not in the other two groups [13,28,29], indicating the presence of strain-specific epitopes on coat proteins of some PVY strains.…”

Section: Introductionmentioning

confidence: 99%

Coat protein of Potyviruses

Shukla

Thomas²,

McKern

et al. 1988

Archives of Virology

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Four strains of potato virus Y, PVY-D, PVY-10, PVY-18, and PVY-43, obtained from different Australian sources were compared on the basis of their biological, serological and coat protein structural properties. Each of the strains could be distinguished on the basis of their reactions on selected test plant species. Two of the PVY strains, PVY-D and PVY-10, induced symptoms similar to those produced by the PVYO strain group. The reactions of PVY-18 and PVY-43, although comparable to PVYN in some hosts, did not completely match the description of the PVYN strain group. In contrast to the other three strains, PVY-18 could not be transmitted by Myzus persicae in repeated tests. No difference was observed in the serological properties of the four PVY strains in different assay systems, using polyclonal antisera. The amino acid sequences of the coat proteins of PVY-10, PVY-18, and PVY-43 were obtained and compared with the coat protein sequences of pepper mottle virus (PeMV) [Dougherty WG, Allison RF, Parks TD, Johnston RE, Feild MJ, Armstrong FB (1985) Virology 146: 282-292] and PVY-D [Shukla DD, Inglis AS, McKern NM, Gough KH (1986) Virology 152: 118-125]. The homology between the PVY strains ranged from 96.3 to 99.3% and with the PeMV sequence, 91.4 to 92.9%. Based on this high sequence homology, and the previous observation that coat protein sequences of potyvirus strains are always greater than 90% identical, PeMV could be considered a strain of PVY. However, PVY and PeMV are reported to be only distantly serologically related and on this basis PeMV is currently considered to be an independent member of the Potyvirus group.

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Diagnosis of potato virus Y^N: a comparison between polyclonal and monoclonal antibodies and a biological assay

Cited by 12 publications

References 5 publications

Characterisation of potato virus Y isolates from Iran

Characterisation of potato virus Y isolates from Iran

New Approaches to the Detection of Microbial Plant Pathogens

Coat protein of Potyviruses

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Diagnosis of potato virus YN: a comparison between polyclonal and monoclonal antibodies and a biological assay

Cited by 12 publications

References 5 publications

Characterisation of potato virus Y isolates from Iran

Characterisation of potato virus Y isolates from Iran

New Approaches to the Detection of Microbial Plant Pathogens

Coat protein of Potyviruses

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Diagnosis of potato virus Y^N: a comparison between polyclonal and monoclonal antibodies and a biological assay