Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization

Martins, Lara Barros; Rosseto, José Henrique Feijó; Andrade, Natália Silva; Braz‐Silva, Paulo Henrique; Ortega, Karem L.

doi:10.1155/2017/3457479

Cited by 8 publications

(12 citation statements)

References 20 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The methods used here are in line with those previously used. Our results are somewhat confusing as other tumour related viruses such as Epstein-Barr and Human papilloma virus have been detected in tumour tissue, both applying IHC and ISH techniques [32, 33]. In our opinion, for a convincing statement on the causal relationship between a virus and a tumour, IHC results should be confirmed with a technique detecting signs of the virus at a genomic or RNA level.…”

Section: Discussionmentioning

confidence: 62%

Expression of CMV protein pp65 in cutaneous malignant melanoma

2019

View full text Add to dashboard Cite

Human cytomegalovirus (CVM) has been detected by immunohistochemistry (IHC) in brain tumours; however, whether CMV antigen is seen in melanomas has not yet been elucidated. Applying IHC, melanoma tissue was assessed for the expression of pp65, a tegument protein of CMV. Two cohorts were available, cohort-I and II, the latter included also related metastasis. In addition to IHC, in situ hybridisation (ISH) was carried out to assess whether CMV related genetic sequences were detectable in a subset of cases. Seventy per cent of the 142 cases in cohort-I and 50% of the 37 cases in cohort-II displayed immunoreactivity (IR). In both cohorts, the IHC outcome correlated with T-stage (Cohort I: Spearman 0.22, p = 0.01, Cohort II: Fisher exact text 0.04). In 30 of cohort-II cases, when IHC staining was carried out on both the primary tumour and the corresponding metastasis, no change in IR was noted in 53%; in 20%, the IR was lower and in 27% higher in the metastasis when compared with the primary tumour. These results were significant (Fisher exact test 0.03). Applying ISH technique on four tumour cases with detectable pp65 protein, CMV related genetic sequence was not detected. Here, we demonstrate, congruent with observations published for brain tumours, that the protein pp65 is indeed observed in substantial number of melanoma cases with IHC; however, no signal was detected with ISH technique. These findings are in line with previously reported studies, demonstrating that the role of CMV in tumours is still debatable.

show abstract

Section: Discussionmentioning

confidence: 62%

Expression of CMV protein pp65 in cutaneous malignant melanoma

2019

View full text Add to dashboard Cite

show abstract

“…is usually related to human immunodeficiency virus (HIV) and other immunocompromised conditions. 1 Herein, we aimed to present a case of OHL and make an emphasis on treatment options. as it may manifest at CD4 T cell counts less than 500/mm 3 .…”

mentioning

confidence: 99%

Treatment options for oral hairy leucoplakia: A case report

Müller

Stoeckle

Goldust

2020

Dermatologic Therapy

View full text Add to dashboard Cite

“…Presence of unilateral or bilateral, hairlike, white plaques on the lateral borders of the tongue (3,5,6,11,12). It is due to Epstein-Barr virus infection in immunosuppressed or immunocompromised patients (3,5,6,11,12). The plaques are adherent and, therefore, cannot be scraped off (1,12).…”

Section: Oral Hairy Leukoplakiamentioning

confidence: 99%

“…It is due to Epstein-Barr virus infection in immunosuppressed or immunocompromised patients (3,5,6,11,12). The plaques are adherent and, therefore, cannot be scraped off (1,12). This pathology is usually asymptomatic (1,12).…”

Section: Oral Hairy Leukoplakiamentioning

confidence: 99%

See 1 more Smart Citation

Hairy Tongue: Differential Diagnosis by Use of Widefield Optical Fluorescence

Andrade

Ribeiro²,

Pratavieira

et al. 2019

Braz. Dent. J.

View full text Add to dashboard Cite

Hairy tongue is a benign pathology, characterized clinically by hyperkeratinized plaques on the dorsal surface of the tongue, hairlike, whose coloration ranges from unpigment, whitish, yellowish, green, brown to black. Diagnosis is clinical, and, in cases of whitish plaques, it may be difficult to differentiate between oral hairy leukoplakia, potentially malignant leukoplakia or squamous cell carcinoma. Thus, widefield optical fluorescence complementary examination may allow a better visualization of the local hairlike pattern of hyperkeratinization, typical of the hairy tongue, facilitating the diagnosis. In this work, a 57-year-old man was referred to the Dental Specialties Department of the Divinópolis Health Department (MG, Brazil) by a general dental practitioner, aiming a differential diagnosis of possible malignant lesion on the dorsal tongue surface. The complementary examination by wide-field optical fluorescence was performed. For this, it was employed a device with high-power light-emitting diode emitting light centered at a wavelength of (400±10) nm and maximum irradiance of (0.040±0.008) W/cm2 was used for fluorescence visualization. Fluorescence images showed projections of hairlike appearance in tongue dorsal surface with no aspects of malignancy. Hairlike appearance is the principal feature of hairy tongue. In this way, the final diagnosis was established. In conclusion, in this case, the use of widefield optical fluorescence in oral diagnostic routine provided a differential diagnosis, with no need of an incisional biopsy.

show abstract

Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization

Cited by 8 publications

References 20 publications

Expression of CMV protein pp65 in cutaneous malignant melanoma

Expression of CMV protein pp65 in cutaneous malignant melanoma

Treatment options for oral hairy leucoplakia: A case report

Hairy Tongue: Differential Diagnosis by Use of Widefield Optical Fluorescence

Contact Info

Product

Resources

About