Diagnosis of influenza viruses with special reference to novel H1N1 2009 influenza virus

Broor, Shobha; Chahar, Harendra Singh; Kaushik, Samander

doi:10.1007/s12088-009-0054-5

Cited by 16 publications

(15 citation statements)

References 14 publications

(14 reference statements)

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“…Virus isolation is the gold standard but has many problems with it [18]. Molecular diagnostic assays are comparatively better than other diagnostic methods for viral agents [19][20][21][22][23][24][25][26][27][28][29]. In general, our study showed male predominance accounting for 64% of all GC cases and the mean age was 58 years suggesting that gastric cancer appears more often in older individuals.…”

Section: Discussionmentioning

confidence: 82%

Study of EBV and Associated Gastric Malignancies

Supraja

Vardarajalu

Karkuzhali

et al. 2021

JPRI

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Introduction: Epstein Barr virus (EBV), is a member of the genus Lymphocryptoviridae and Herpesviridae subfamily. It is pervasive and infects more than 90% of the adult population worldwide. In childhood and early adulthood primary infection occurs through salivary contact. The majority of children are asymptomatic, but some adolescents and young adults can develop infectious mononucleosis with harmless clinical manifestations. Objective: To analyze the expression of Epstein Barr Virus (EBV) Latent membrane protein – 1 (LMP-1) in various histological subtypes of gastric carcinoma. Materials and Methods: Out of the 43cases, 32 cases were proven to be malignant, out of which 30 cases who had adequate clinical data were included in the study. The materials were processed and sections were cut at 5 microns. Hematoxylin and eosin staining of sections was done. Histopathological examination of these sections was done. Result: The most common presenting symptom associated with Gastric carcinoma was upper abdominal pain (epigastric pain). Both the LMP-1 IHC positive cases showed a low score when assessed using the LMP-1 immunohistochemistry scoring system (used in nasopharyngeal carcinomas). This may be due to the older age group of patients. For the effective control of viral cancer, there should be rapid, sensitive, specific, cost-effective diagnostics assays and management. Conclusion: Hence our study justifies the role of EBV in the oncogenesis of gastric carcinoma. More elaborate and extensive studies are warranted to further emphasize this theory.

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Section: Discussionmentioning

confidence: 82%

Study of EBV and Associated Gastric Malignancies

Supraja

Vardarajalu

Karkuzhali

et al. 2021

JPRI

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“…For the effective control of a viral outbreak, there should be a rapid, sensitive, specific and cost-effective diagnosis assay should be present. Molecular diagnosis assays are the best diagnostic methods as compared with other methods [ 2 , 4 , 7 , 8 , 31 ]. Dengue is still a big challenge for most of the countries and has become a global health issue.…”

Section: Discussionmentioning

confidence: 99%

In-vitro and in silico activity of Cyamopsis tetragonoloba (Gaur) L. supercritical extract against the dengue-2 virus

et al. 2020

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Our health and wealth are highly influenced by a number of viruses. Dengue is one of them having a global influence in absence of vaccines and antiviral. WHO suggested that the morbidity of dengue is increasing more than 6 times from 0.5 million in 2010 to over 3.34 million in 2016, following a sharp increase in 2019. The aim of the present study is to check the in vitro and in silico antidengue activity of Cyamopsis tetragonoloba supercritical extract in cell lines. The optimum yield of supercritical extract was obtained 0.13 g/10 g (1.3% w/w) at 40°C temp and 15 MPa pressure and further characterized by GC-MS. The antiviral assay was performed on C6/36 cell lines with 100 copies of dengue-2 virus and maximum nontoxic dose (31.25 lg/ml) of supercritical extract and their effect was detected by real-time RT-PCR. This study revealed that C. tetragonoloba supercritical extract inhibited the dengue-2 virus (99.9%). GC-MS analysis of C. tetragonoloba supercritical extract showed the presence of 10 compounds. The major compounds identified were Hexadecanoic acid, 15-methyl-methyl ester (24.498%); 9,12-octadecadienoyl chloride, (z,z)-(23.718%); methyl dodecanoic acid (13.228%); methyl-stearate (8.696%); Tridecanoic acid, 12-methyl-, methyl-ester (8.426%), dodecanoic acid (6.102%). The study reveals that C. tetragonoloba can be exploited to develop an effective, inexpensive, and specific anti-dengue. The molecular docking study demonstrated the binding energy of 1,2benzenedicarboxylic acid, bis(2-methylpropyl) ester (-4.1 kcal/mol), 9,12-octadecadienoyl chloride (z,z) (-4.0 kcal/mol) ligands were higher than others. It is concluded that C. tetragonoloba can play a major role to inhibit dengue-2 virus.

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“…Current prevalent techniques to reliably screen for and clinically diagnose the H1N1 virus include direct antigen detection tests, virus isolation in cell culture or detection of influenza-specific RNA by real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR). 9 Here, we demonstrate the application of PMs for specific and sensitive detection of the H1N1 HA Peptide as an example of both antigen-antibody binding and the H1N1 virus marker detection.…”

Section: Author(s) All Article Content Except Where Otherwise Notedmentioning

confidence: 96%

Piezoresistive measurement of Swine H1N1 Hemagglutinin peptide binding with microcantilever arrays

et al. 2014

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Effective detection of Swine H1N1 Hemagglutinin peptide is crucial as it could be used as a positive control to screen for highly infectious flu strains such as Swine-Origin Influenza A (H1N1). Piezoresistive microcantilever arrays present a pathway towards highly sensitive and label-free detection of biomolecules by transducing the antigen-antibody binding into change in resistivity via induced surface stress variation. We demonstrate a mechanical transduction of Swine H1N1 Hemagglutinin peptide binding and suggest the employed technique may offer a potential platform for detection of the H1N1 virus, which could be clinically used to diagnose and provide subsequent relief.

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Diagnosis of influenza viruses with special reference to novel H1N1 2009 influenza virus

Cited by 16 publications

References 14 publications

Study of EBV and Associated Gastric Malignancies

Study of EBV and Associated Gastric Malignancies

In-vitro and in silico activity of Cyamopsis tetragonoloba (Gaur) L. supercritical extract against the dengue-2 virus

Piezoresistive measurement of Swine H1N1 Hemagglutinin peptide binding with microcantilever arrays

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