Diagnosis of IgE-mediated reactions to food antigens by radioimmunoassay

Hoffman, Donald R.; Haddad, Zack H.

doi:10.1016/0091-6749(74)90053-0

Cited by 104 publications

(32 citation statements)

References 13 publications

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“…For instance, Hoff man and Haddad [10] were able to detect by radio-im munoassay IgE antibody against /i-lactoglobulin in only a few milk-sensitive patients' sera. And Kletteret al [I I], using a similar technique, failed to detect IgE antibodies against /J-lactoglobulin in the circulations of a group of infants under I year of age who had been fed cow's milk.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Allergenicity of β-Lactoglobulin and Its Cleavage Fragments

Huang¹,

Coleman²,

Stanworth³

1985

Int Arch Allergy Immunol

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Fragments of β-lactoglobulin were produced by proteolytic cleavage with trypsin, and chemical cleavage with cyanogen bromide, followed by gel filtration on Sephadex G-50. The antigenicity and allergenicity of the products were studied, before and after reductive cleavage by treatment with 2-mercaptoethanol. The former was determined by inhibition ELISA using IgG anti-β-lactoglobulin raised in rabbits, whilst the latter was determined by inhibition ELISA and mast cell challenge, using respectively the sera and peritoneal cells of rats experimentally sensitised to β-lactoglobulin. The findings raise interesting points about the structural basis of allergenicity in relation to antigenicity.

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Section: Discussionmentioning

confidence: 99%

Investigation of the Allergenicity of β-Lactoglobulin and Its Cleavage Fragments

Huang¹,

Coleman²,

Stanworth³

1985

Int Arch Allergy Immunol

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“…After further washing in 0.15 M NaCI, the discs were placed in plastic tubes (16 x 125 mm (17)), and the radioactivity bound to the discs was determined in a Nuclear Chicago Ultrascaler I1 gamma counter. Disintegrations per minute were compared with standard controls according to the method of Hoffman and Haddad (4). In this procedure, the nonspecific binding of lZSI-anti-lgE was determined for each type of allergen disc without prior incubation with serum.…”

Section: Radioallergosorbent Testmentioning

confidence: 99%

Serum Immunoglobulin E Concentrations and Radioallergosorbent Tests in Children with Atopic Dermatitis

1976

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ExtractSerum IgE concentrations and the presence of allergen-specific IgE were determined in a series of 23 children with atopic dermatitis. In this group 83% had significantly elevated serum levels of IgE, 91% had coexistent respiratory allergy, 70% had radioallergosorbent test (RAST) evidence of pollen hypersensitivity, and 43% gave a history and demonstrated a RAST score consistent with milk or egg hypersensitivity. In patients with eczema a significant proportion of the elevated serum IgE appears to be antigen specific. SpeculationThe mechanisms involved in the pathogenesis of atopic dermatitis remain unknown. Elevated serum IgE and the presence of antigen specific IgE suggest a causal relationship of atopic eczema with immediate hypersensitivity. Alternatively, the disease process may ,have a marked stimulatory effect on the specific IgE response. The precise nature of this association remains to be determined.Atopic dermatitis or eczema has been defined as an allergic dermatosis which is characterized by erythema, intense itching, and vesiculation (3). Although the pathogenesis is unknown, the strong association of atopic dermatitis, asthma, hay fever, and family history of atopic disease suggests that immunologic mechanisms are frequently involved. Elevated serum IgE levels have been reported frequently in atopic dermatitis, (1,(6)(7)(8)10) and the concentration of IgE appears to be correlated with the severity of the dermatitis (6, 7). Further, Hoffman et al. (5,6) have recently demonstrated specific IgE to a wide variety of antigen in patients with eczema.The present study was undertaken to evaluate serum IgE levels in a selected group of pediatric patients seen in an allergy clinic to determine the presence or absence of allergen-specific IgE and to examine the relation of atopic dermatitis to inhalant and food allergies. SUBJECTS AND METHODS PATIENT POPULATIONThe patient population consisted of 23 children ranging in age from I to 15 years with varying degrees of symptomatic atopic dermatitis seen at the Georgetown University Allergy-lmmunology-Respiratory Disease (AIR) Clinic (Table 1). There were 10 males and 13 females in the study group. In order to examine the relationships of age and IgE, the patients were divided into four age categories: 9 were 1-4 years of age, 4 were 5-8 years of age, 6 were 9-12 years of age, and 4 were 13-15 years of age. CLASSIFICATION OF DISEASEAtopic dermatitis was classified as mild, moderate, or severe according to the extent of the eruption at the time serum samples were obtained. 'Dermatitis of limited distribution with few or no active lesions was classified as "mild." Dermatitis limited to the face, neck, or flexor surfaces of the extremities with limited erythema and vesidulation was "moderate." Extensive involvement of the trunk and extensor surfaces, severe lichenification, extensive erythema, and vesiculation or any of these in combination were classified as "severe."For the purpose of this study, allergic rhinitis or asthma was designated as present (+) or a...

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“…Seafood, including Crustacea, are among the most frequently mentioned foods which are claimed to cause adverse reactions in food-sensitive individuals [1][2][3][4][5]. Adverse reactions to foods may be mediated by nonimmunologic mechanisms, such as natural toxins, contaminants and preservatives present in the food, as well as by immunologic mechanisms [3].…”

Section: Introductionmentioning

confidence: 99%

Crawfish and Lobster Allergens: Identification and Structural Similarities with Other Crustacea

Halmepuro¹,

Salvaggio²,

Lehrer³

1987

Int Arch Allergy Immunol

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Antigenic and allergenic components in crawfish and lobster extracts were studied using crossed immunoelectrophoretic techniques. Crossed immunoelectrophoresis with rabbit antisera revealed 23 antigens in crawfish and 17 antigens in lobster extracts. Both extracts exhibited structural similarities in antigens mutually and with other Crustacea in crossed-line immunoelectrophoresis. Crossed radioimmunoelectrophoresis (CRIE) demonstrated 6 crawfish and 4 lobster allergens when individual or pooled sera from radioallergosorbent test (RAST)-positive crustacea-sensitive subjects were used. Since radiostaining was also observed with sera from RAST-negative nonsensitive subjects, specificity of IgE binding was tested using CRIE-inhibition. Preincubation of RAST-positive sera with crawfish or lobster extract decreased radiostaining in CRIE, while no changes occurred when using control sera. These results confirmed the presence of IgE-mediated mechanisms in seafood allergy and demonstrated a number of shared antigenic determinants among Crustacea allergens.

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Diagnosis of IgE-mediated reactions to food antigens by radioimmunoassay

Cited by 104 publications

References 13 publications

Investigation of the Allergenicity of β-Lactoglobulin and Its Cleavage Fragments

Investigation of the Allergenicity of β-Lactoglobulin and Its Cleavage Fragments

Serum Immunoglobulin E Concentrations and Radioallergosorbent Tests in Children with Atopic Dermatitis

Crawfish and Lobster Allergens: Identification and Structural Similarities with Other Crustacea

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