“…The RAST technique was used as a diagnosis method, where the positive skin test patient sera were evaluated using a crude extract of the potential allergenic seafood, such as in crustaceans, prawn, shrimp, crawfish, and lobster (Daul et al, 1988;Halmepuro et al, 1987;Nagano et al, 1984). This technique was used to study the cross-reactivity between the oyster and crustacean (Lehrer & McCants, 1987) and caddis fly with arthropoda and mollusca (Koshte et al, 1989), which indicated to the non-specificity of RAST to be a diagnostic assay.…”