Diagnosis of <i>Helicobacter pylori</i> Infection

Krogfelt, Karen A.; Lehours, Philippe; Mégraud, Françis

doi:10.1111/j.1523-5378.2005.00341.x

Cited by 50 publications

(41 citation statements)

References 77 publications

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“…Heterogeneous results have been reported concerning the performance of the rapid ImmunoCard STAT! HpSA test in pretreatment versus posttreatment patients (1,7,9). The difference could also be due to the higher percentage of males (70%) than females (30%) than in our investigation (49% and 51%, respectively).…”

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confidence: 42%

“…While the value of enzyme immunoassays (EIAs) using either polyclonal or monoclonal antibodies is well documented, the sporadic existing data for the first developed and commercially available rapid test (ImmunoCard STAT! HpSA) showed enormous differences among the studies (1,7,8,14,17), and its reliability is reported to be somewhat lower than that of monoclonal fecal antigen EIA (2,9).…”

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confidence: 99%

“…In the last years interest has been focused on noninvasive methods, especially the detection of the pathogen in feces. The H. pylori stool antigen test provides a simple alternative to the urea breath test and is appropriate for diagnosis and follow-up of infection (3,9,11,12,14,(16)(17)(18). While the value of enzyme immunoassays (EIAs) using either polyclonal or monoclonal antibodies is well documented, the sporadic existing data for the first developed and commercially available rapid test (ImmunoCard STAT!…”

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confidence: 99%

“…No data are available on its reliability as a screening test for primary diagnosis. Moreover, the results of stool antigen tests may vary according to geographical locations (3,9,12). The objective of this trial was to evaluate the performance of this novel test with a group of dyspeptic adults in our region, in comparison to a well-defined H. pylori status established by invasive diagnostic methods.…”

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Evaluation of a Rapid New Stool Antigen Test for Diagnosis of Helicobacter pylori Infection in Adult Patients

2008

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The evaluation of a new rapid stool antigen test showed different levels of sensitivity for final readings of test results at 20 min (59.1%) and 30 min (76.9%). Significant differences in performance were observed between the two sexes and the various age categories, with higher efficiency in male patients and young adults. Generally, this test is efficient and can be used to detect H. pylori infection in adults. However, further studies are required to confirm its accuracy.Helicobacter pylori is well recognized as a major cause of gastrointestinal diseases (10,11). Patients with successful eradication therapy show evidence for this cause-and-effect relationship; gastritis and ulcers often are cured, and the risk of recurrence is greatly reduced (4). Therefore, reliable detection of H. pylori infection is of major importance. In the last years interest has been focused on noninvasive methods, especially the detection of the pathogen in feces. The H. pylori stool antigen test provides a simple alternative to the urea breath test and is appropriate for diagnosis and follow-up of infection (3,9,11,12,14,(16)(17)(18). While the value of enzyme immunoassays (EIAs) using either polyclonal or monoclonal antibodies is well documented, the sporadic existing data for the first developed and commercially available rapid test (ImmunoCard STAT! HpSA) showed enormous differences among the studies (1,7,8,14,17), and its reliability is reported to be somewhat lower than that of monoclonal fecal antigen EIA (2, 9).Rapid Hp StAR (DakoCytomation Ltd., United Kingdom) is a newly developed qualitative immunochromatographic membrane-based assay using monoclonal antibodies and amplification technology for the direct detection of H. pylori antigens in human feces. The test has two capture lines, one coated with an H. pylori-specific amplified capture reagent (test line) and one with a control capture reagent (control line). A recent study reported good performance with posttreatment patients (14). No data are available on its reliability as a screening test for primary diagnosis. Moreover, the results of stool antigen tests may vary according to geographical locations (3, 9, 12). The objective of this trial was to evaluate the performance of this novel test with a group of dyspeptic adults in our region, in comparison to a well-defined H. pylori status established by invasive diagnostic methods. To determine sources of heterogeneity that may have an influence on applicability, the test was evaluated in relation to clinical manifestations and the ages and genders of the patients.(The results of this study were presented in part at the 17th European Congress of Clinical Microbiology and Infectious Diseases, Munich, Germany, 2007.) A total of 72 consecutive patients (37 females and 35 males) (mean age, 58.4 Ϯ 12 years; range, 24 to 88 years) who were referred to the Department of Surgery at the University Hospital of Kiel and to a gastroenterological private practice were enrolled in the study between 2002 and 2003. All patients who pre...

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confidence: 99%

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confidence: 99%

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Evaluation of a Rapid New Stool Antigen Test for Diagnosis of Helicobacter pylori Infection in Adult Patients

2008

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“…Therefore, stool PCR may be considered as better test than HpSA because the HpSA test shows heterogeneity in the sensitivity rates that ranged from 58% to 96% and specificity ranged from 67% to96 % [14][15][16][17][18][19][20][21][22]. This is because of the difficulty in obtaining polyclonal antibodies of constant quality [23].…”

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confidence: 99%

Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection

Mishra

Singh

Jain

et al. 2008

J Infect Dev Ctries

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Background: Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection. Methodology: A total of 52 patients with dyspeptic symptoms were included in the study. Antral biopsy was collected during preand post-therapy periods for rapid urease test (RUT) and PCR. Similarly stool specimens for PCR and HpSA test were collected during both the periods from all 52 patients. Biopsy, PCR and RUT results together were considered the "gold standard." Results: On the basis of gold standard tests, 40/52 patients were H. pylori positive. The sensitivity and specificity of HpSA test were 80% and 83.3% respectively in untreated patients. On the other hand, the sensitivity and specificity of stool PCR in untreated patients were 72.5% and 100% respectively. After eradication therapy, the results of both RUT and biopsy PCR were negative in 87.5% and positive in 12.5% of the patients. Although post treatment sensitivity of HpSA and stool PCR was equal (60%), specificity of HpSA and stool PCR were 68.6% and 97.1% respectively. Conclusion: The H. pylori stool tests represent a non-invasive concept for diagnosis of infection. Both HpSA and stool PCR seem to be satisfactory tests for pre-eradication as well as assessment of infection. But stool PCR is a better indicator than HpSA test in the post-eradication assessment of infection.

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A Diagnostic Device for Helicobacter pylori Infection

Mandenius

Björkman

2011

Biomechatronic Design in Biotechnology

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Diagnosis of Helicobacter pylori Infection

Cited by 50 publications

References 77 publications

Evaluation of a Rapid New Stool Antigen Test for Diagnosis of Helicobacter pylori Infection in Adult Patients

Evaluation of a Rapid New Stool Antigen Test for Diagnosis of Helicobacter pylori Infection in Adult Patients

Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection

A Diagnostic Device for Helicobacter pylori Infection

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