Diagnosis of Human Giardiasis

Smith, H. V.; Mank, T. G.

doi:10.1007/978-3-7091-0198-8_22

Cited by 11 publications

(12 citation statements)

References 60 publications

(24 reference statements)

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“…This may be due to the similarity of population condition in both studies. In agreement with previous studies [8], our study also revealed the highest levels of G. intestinalis infection in children ≤10 years old and then in the 21-30 age group. Children in developing countries are more likely to be exposed to G. intestinalis infection due to poor public health conditions.…”

Section: Discussionsupporting

confidence: 93%

Prevalence of Intestinal Parasitic Infections among Patients Referring to Medical Diagnostic Laboratories, Kashan, Central Iran, 2015-2018

Khodabakhsh-Arbat

Hooshyar

Sadeghi

2018

JoMMID

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Intestinal parasitic infections are among the most common human infections worldwide and are considered as critical health indicators of a community, especially in developing countries [1]. According to the World Health Organization (WHO) report, more than two billion people in the world are infected with intestinal parasitic infections [2-3]. More than 1.5 billion (24%) of the world's population are estimated to be infected with parasitic infections such as Ascaris lumbricoides, hookworms and Trichuris trichiura [4]. These infections are widespread mainly in tropical and subtropical regions. The highest incidences are reported from developing countries, more in sub-Saharan Africa, North and South America, China, and East Asia, where people are struggling with health problems, insufficient water supply, rapid population growth and other economic and social problems [4-6]. The most prominent pathogenic protozoa of human intestine are Giardia intestinalis, Entamoeba histolytica/Entamoeba dispar, Dientamoeba fragilis and Blastocystis sp. [3, 6]. Giardia intestinalis is one of the most common etiological agents of parasitic diarrhea in humans and animals. This parasite has a global distribution, and it is estimated that 280 million people are affected by this parasite worldwide [7]. The global prevalence of this protozoon in human has been estimated to be about 8 to 30% in developing countries and 1 to 8% in developed countries [8]. In Iran, like other developing countries, parasitic diseases in communities are considered

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Section: Discussionsupporting

confidence: 93%

Prevalence of Intestinal Parasitic Infections among Patients Referring to Medical Diagnostic Laboratories, Kashan, Central Iran, 2015-2018

Khodabakhsh-Arbat

Hooshyar

Sadeghi

2018

JoMMID

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“…It has been also associated with impaired growth and cognitive function in poor resource settings [ 5 – 7 ] and developed countries [ 8 ]. Human giardiasis prevalence in developed nations ranges from 1 to 8% of the population; however, in developing countries, it exceeds 30% of the population [ 9 ]. The prevalence rate in Egypt is up to 30.2% [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Genotyping of Giardia duodenalis in children in upper Egypt using assemblage- specific PCR technique

Ahmad¹,

El-Kady²,

Hassan³

2020

PLoS ONE

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Giardia duodenalis is a common gastrointestinal protozoan parasite, causing diarrheal illness in humans worldwide. Yet, the distribution of G. duodenalis genotypes among human patients and their clinical relevance remains controversial. This study aimed to detect G. duodenalis in children in Upper Egypt and identify causative genotypes and elucidate a possible correlation between genotype and clinical presentation. One hundred sixty-five children, regardless of symptoms, were tested for giardiasis. Giardia positive stool samples (40/165) were subjected to PCR amplification targeting the tpi gene with positive PCR results in only 35 cases (87.5%). Assemblage-specific amplification of genotypes (A, B, and the zoonotic E strains) revealed predominantly G. duodenalis Assemblage A (45.7%). Assemblage B and mixed A and B infections were detected in 31.4% and 22.8% of children, respectively. Assemblage E was not detected. G. duodenalis assemblage A was dominant in children who complained of diarrhea and abdominal cramps. In contrast, asymptomatic children with positive stool samples display a higher frequency of assemblage B and mixed infections. The study highlights the predominance of Giardia Assemblage A in our study locality. This study is the first for this endemic area to use the copro-PCR technique for diagnosis and genotyping of giardiasis. Study results show the value of simple species-specific primers for genotyping in communities with little access to laboratory resources. Further genetic studies are needed to clarify the association between parasite genetic diversity and patient symptomatology.

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“…The upper three layers were discarded, and the sediment was moved to a slide, covered with a cover slip and analysed under a microscope using magnifications 10X and 40X. This was as per the protocol by Smith & Mank (2011) .…”

Section: Methodsmentioning

confidence: 99%

The relationship between Helicobacter pylori infection and intestinal parasites in individuals from Khartoum state, Sudan: a case-control study

2019

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In developing countries, infection is Background:Helicobacter pylori common, as are intestinal parasites. Socioeconomic circumstances and low personal hygiene lead to the spread of these infections. This research aimed to evaluate the relationship between intestinal parasites and H. pylori in Khartoum, Sudan.This study was conducted in various hospitals in Khartoum Methods: between June and October 2018. The study involved 200 individuals: 100 patients with as a case group and 100 healthy individuals as a H. pylori control group. A stool sample was taken from each individual, and wet preparation, saturated sodium chloride flotation and formal ether concentration were used to detect intestinal parasites.The results showed that 23% of patients and 10% of Results:H. pylori healthy individuals had gastrointestinal parasites; Entamoeba histolytica was found in 12% of cases followed by (7%) and H. pylori Entamoeba coli (4%). Control group:in 5% followed Giardia lamblia Entamoeba histolytica by in 3% and E. coli in 2% of individuals. There was a significant G. lamblia difference in the prevalence of intestinal parasites between groups (P = 0.013). The prevalence rate of intestinal parasites among men and women was 24% and 22%, respectively, in the case group, and 9% and 11%, respectively, in the control group. In the case group, the highest prevalence rates (40% and 38%) were found among the age groups 1-15 and 46-60 years old, respectively, while the lowest rate (10.7%) was found among the 31-45 age group. In the control group, the highest prevalence rate (15%) was among the 31-45 age group, the lowest prevalence rate (8%) was found among the 16-30 age group.Together, we found that intestinal parasites are more Conclusion: common in patients with . We also noticed that the rate of infection H. pylori was not affected by gender while the age group was affected.

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Diagnosis of Human Giardiasis

Cited by 11 publications

References 60 publications

Prevalence of Intestinal Parasitic Infections among Patients Referring to Medical Diagnostic Laboratories, Kashan, Central Iran, 2015-2018

Prevalence of Intestinal Parasitic Infections among Patients Referring to Medical Diagnostic Laboratories, Kashan, Central Iran, 2015-2018

Genotyping of Giardia duodenalis in children in upper Egypt using assemblage- specific PCR technique

The relationship between Helicobacter pylori infection and intestinal parasites in individuals from Khartoum state, Sudan: a case-control study

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