Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques

Parkash, Om; Shueb, Rafidah Hanim

doi:10.3390/v7102877

Cited by 93 publications

(78 citation statements)

References 81 publications

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“…As shown in Table 1, similar to that of the reference qRT-PCR for all four serotypes, the sensitivity of the RT-iiPCR system was at biological titers of around 10 0 PFU/ml. This was consistent to the performance of other molecular detection methods for DENV [37][38][39]. As reported previously, RNA copy numbers were likely significantly higher than PUFs, due to defective virus particles or viral RNA freed from infected cells in the sample matrix [40][41][42].…”

Section: Discussionsupporting

confidence: 90%

An RT-PCR panel for rapid serotyping of dengue virus serotypes 1 to 4 in human serum and mosquito on a field-deployable PCR system

et al. 2019

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BackgroundDengue fever, a mosquito-borne disease, is caused by dengue virus (DENV) which includes four major serotypes (DENV-1, -2, -3, and -4). Some serotypes cause more severe diseases than the other; severe dengue is associated with secondary infections by a different serotype. Timely serotyping can provide early warning of dengue epidemics to improve management of patients and outbreaks. A mobile insulated isothermal PCR (iiPCR) system is available to allow molecular detection of pathogens near points of need.Methodology/Principle findingsIn this study, side-by-side comparison with the CDC DENV-1-4 Real Time RT-PCR (qRT-PCR) was performed to evaluate the performance of four singleplex DENV-1–4 serotyping reverse transcription-iiPCR (RT-iiPCR) reagents for DENV subtyping on the mobile PCR system. The four RT-iiPCRs did not react with Zika virus and chikungunya virus; tests with serial dilutions of the four DENV serotypes made in human serum showed they had detection endpoints comparable to those of the reference method, indicating great analytical sensitivity and specificity. Clinical performance of the RT-iiPCR reagents was evaluated by testing 40 serum samples each (around 20 target serotype-positive and 20 DENV-negative); all four reagents had high agreement (97.5–100%) with the reference qRT-PCR. Moreover, testing of mosquitoes separately infected experimentally with each serotype showed that the four reagents detected specifically their target DENV serotypes in mosquito.Conclusions/SignificanceWith analytical and clinical performance comparable to the reference qRT-PCR assay, the four index RT-iiPCR reagents on the field-deployable PCR system can serve as a useful tool for DENV detection near points of needs.

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Section: Discussionsupporting

confidence: 90%

An RT-PCR panel for rapid serotyping of dengue virus serotypes 1 to 4 in human serum and mosquito on a field-deployable PCR system

et al. 2019

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“…DENV is surrounded by an envelope, which encloses ss‐positive sense ∼1,100 nucleotide base RNA. Translation of viral RNA produces a single polypeptide, which upon the proteolytic cleavage by proteases results in the formation of three structural proteins (capsid, membrane and envelope) and seven non‐structural (NS) proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5) . Currently, the dengue diagnostic is performed using hemagglutination inhibition (HI) assays, ELISAs that detect the presence of IgM or IgG against the virus or DENV NS1 Antigen .…”

Section: Tropical Diseasesmentioning

confidence: 99%

“…Translation of viral RNA produces a single polypeptide, which upon the proteolytic cleavage by proteases results in the formation of three structural proteins (capsid, membrane and envelope) and seven non‐structural (NS) proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5) . Currently, the dengue diagnostic is performed using hemagglutination inhibition (HI) assays, ELISAs that detect the presence of IgM or IgG against the virus or DENV NS1 Antigen . This type of assay suffers three limitations: antibodies are not detectable after 5 days of infection, some patients do not show IgM in the secondary infection, and they are prone to false positives .…”

Section: Tropical Diseasesmentioning

confidence: 99%

“…[79] Currently, the dengue diagnostic is performed using hemagglutination inhibition (HI) assays, ELISAs that detect the presence of IgM or IgG against the virus or DENV NS1 Antigen. [79] This type of assay suffers three limitations: antibodies are not detectable after 5 days of infection, some patients do not show IgM in the secondary infection, and they are prone to false positives. [2] For instance, flavivirus producing yellow fever shares a similar protein structure with dengue.…”

Section: Diseases Caused By Virusesmentioning

confidence: 99%

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Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

2017

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Rapid and reliable diagnosis of viral infections and tropical diseases and timely initiation of appropriate treatment are essential for their successful clinical management. This Review summarizes some basic concepts regarding the main viral infection and tropical diseases to which society is facing today and the conventional methods available for their detection. The tremendous potential offered by electrochemical affinity biosensors to address this important challenge, meeting the required demands for viral infections and tropical diseases diagnosis, is clearly stated by discussing challenges, opportunities, implementation, and application of selected examples focused on the determination of specific biomarkers of different molecular (genetic, regulatory, and functional) levels. The highlighted approaches demonstrate the use of a plethora of specific receptors and assay formats and a great diversity of attractive electrochemical approaches in this rapidly advancing and highly interesting field.

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“…El desarrollo de ensayos biosensores podrían superar las limitaciones del ELISA ya que la tecnología biosensora tiene sensibilidad similar o incluso más alta con la ventaja de portabilidad y miniaturización, además de ser fácil de usar y barata en comparación; sin embargo, aún queda un camino largo para su comercialización. 28 …”

Section: Diagnósticounclassified

Arbovirus en Latinoamérica

2016

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ResumenLas enfermedades producidas por arbovirus son un problema muy grave a nivel mundial, debido a que su vigilancia y prevención implica también la vigilancia de sus vectores, lo que hace difícil su control y casi imposible evitar su expansión a cualquier país tropical o subtropical, e incluso algunas regiones templadas. A nivel mundial, el dengue es la enfermedad por arbovirus más común con 40% de la población mundial viviendo en zonas de transmisión del virus del dengue. De las 390 millones de infecciones estimadas y 100 millones de casos anuales, una proporción pequeña de estos casos progresa a dengue grave. Aproximadamente uno de cada 2,000 casos de dengue causan la muerte; sin embargo, la tasa de letalidad de los pacientes con dengue grave se puede reducir de casi 10% a menos del 0.1% si se actúa rápidamente y con calidad en la atención clínica que reciben los pacientes. Esto, aunado a la reciente y rápida propagación del virus chikungunya y el brote de virus zika en países latinoamericanos, como México, hace necesaria la actualización médica y revisión de bibliografía respecto a la prevención, control y gestión de las infecciones causadas por arbovirus. Por lo tanto, presentamos una revisión de lo más reciente sobre enfermedades por arbovirus en América Latina. Arbovirus in Latin AmericaAbstract Diseases produced by arboviruses are a major worldwide problem due to the fact that the surveillance and prevention of them implies the surveillance of vectors, which makes it very difficult to control them, and almost impossible to avoid their expansion to any tropical or subtropical country, and even some temperate regions. Globally, dengue is the most common arboviral disease, with 40% of the world population living in areas with dengue virus transmission. Of the estimated 390 million infections and 100 million cases annually, a small proportion of these cases progress to severe dengue. Approximately one in 2,000 dengue cases result in death. However, the case-fatality rate of patients with severe dengue can be reduced from almost 10% to less than 0.1% by the timing and quality of clinical care that patients receive. This, coupled with the recent and quick spread of Chikungunya virus and the outbreak of Zika virus in Latin American countries including Mexico, makes it necessary the medical update and review of bibliography concerning the prevention, control and management of arboviral-caused diseases. Therefore we present a review of the most recent literature on arboviral diseases in Latin America.

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Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques

Cited by 93 publications

References 81 publications

An RT-PCR panel for rapid serotyping of dengue virus serotypes 1 to 4 in human serum and mosquito on a field-deployable PCR system

An RT-PCR panel for rapid serotyping of dengue virus serotypes 1 to 4 in human serum and mosquito on a field-deployable PCR system

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

Arbovirus en Latinoamérica

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