Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI‐D) resistance by primer‐introduced restriction enzyme analysis in scytalone dehydratase gene of <i>Magnaporthe grisea</i>

Kaku, Koichiro; Takagaki, Makiichi; Shimizu, Tsutomu; Nagayama, Kozo

doi:10.1002/ps.742

Cited by 43 publications

(36 citation statements)

References 8 publications

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“…Approximately 20 rice blast lesions were collected from each generation. The frequency of the V75M-mutation in the Sdh1 gene of rice blast in each generation was analyzed by primer-introduced restriction enzyme analysis PCR (PIRA-PCR), following the method of Kaku et al (2003).…”

Section: Stability Of Mbi-d-resistant Isolates Of M Oryzae During Sumentioning

confidence: 99%

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Fitness Studies Between Field Isolates of Magnaporthe oryzae Resistant and Sensitive to Scytalone Dehydratase Inhibitors of Melanin Biosynthesis (Mbi-Ds) and Decline of Frequency of Resistant Isolates at a Rice Field

Kimura

Fujimoto

2015

J Plant Dis Prot

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Fitness of isolates of Magnaporthe oryzae resistant to scytalone dehydratase inhibitors of melanin biosynthesis (MBI-Ds) was studied in comparison with sensitive isolates. We found no significant differences in pathogenicity between sensitive and resistant isolates. In competition studies, sensitive isolates became dominant after four to six successive inoculations from the diseased rice seedlings infected by both sensitive and resistant isolates, irrespective of the initial ratio of these isolates. No revertants were detected after ten successive inoculations from diseased rice seedlings. Besides, at one rice paddy field, the frequency of resistant isolates declined dramatically after stopping the MBI-Ds applications. These results support our hypothesis that resistant isolates have lower fitness than sensitive isolates.

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Section: Stability Of Mbi-d-resistant Isolates Of M Oryzae During Sumentioning

confidence: 99%

“…1). Approximately 20 rice blast lesions were collected from infected seedlings after each cycle for the detection of the frequency of the V75M-mutation in the Sdh1 gene by PIRA-PCR (Kaku et al 2003). Data between 1 st generation and last generation were compared in X 2 analysis (P < 0.05).…”

Section: Competition Between Sensitive and Resistant Isolatesmentioning

confidence: 99%

Fitness Studies Between Field Isolates of Magnaporthe oryzae Resistant and Sensitive to Scytalone Dehydratase Inhibitors of Melanin Biosynthesis (Mbi-Ds) and Decline of Frequency of Resistant Isolates at a Rice Field

Kimura

Fujimoto

2015

J Plant Dis Prot

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“…Alternatively, fungal DNA was extracted using a microwave, then used directly for PCR as described by Kaku et al (2003).…”

Section: Fungal Isolates and Dna Extractionmentioning

confidence: 99%

“…This MBI-D resistance was caused by a single point mutation in the fungicide target gene for scytalone dehydratase (SCDH) Yamada et al 2004). Subsequently, Kaku et al (2003) developed a method for detecting MBI-D resistance in M. oryzae isolates using a primer-introduced restriction enzyme analysis polymerase chain reaction (PIRA-PCR). This method, in which the PCR products from resistant isolates are specifically recognized after treatment with restriction enzyme XbaI results in two bands on an agarose gel, is now commonly used.…”

Section: Introductionmentioning

confidence: 99%

First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens

et al. 2008

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Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein.PCR-Luminex, a novel system developed for highthroughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences.

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“…Thus, primer-introduced restriction analysis PCR (PIRA-PCR) has become a useful method to create diagnostic artificial RFLPs (Haliassos et al, 1989). A PIRA-PCR assay was developed for detecting carpropamid resistant Magnaporthe grisea (Kaku et al, 2003).…”

Section: Pcr-rflp and Primer-introduced Restriction Analysis Pcr (Pirmentioning

confidence: 99%

Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance

Capote¹,

Pastrana²,

Aguado³

et al. 2012

Plant Pathology

103

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Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI‐D) resistance by primer‐introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea

Abstract: We have established a simple diagnosis method for rice blast fungus resistant to MBI-D. This involves the preparation of PCR templates directly from the lesions in combination with primer-introduced restriction enzyme analysis PCR (PIRA-PCR).

Cited by 43 publications

References 8 publications

Fitness Studies Between Field Isolates of Magnaporthe oryzae Resistant and Sensitive to Scytalone Dehydratase Inhibitors of Melanin Biosynthesis (Mbi-Ds) and Decline of Frequency of Resistant Isolates at a Rice Field

Fitness Studies Between Field Isolates of Magnaporthe oryzae Resistant and Sensitive to Scytalone Dehydratase Inhibitors of Melanin Biosynthesis (Mbi-Ds) and Decline of Frequency of Resistant Isolates at a Rice Field

First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens

Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance

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