Diagnosis of congenital toxoplasmosis by polymerase chain reaction

Rasti, Sima; Behrashi, Mitra; Kazemi, Bahram; Fatahian, A; Mousavi, Gholamabbas; Namakchian, M

doi:10.4103/0255-0857.96725

Indian Journal of Medical Microbiology

2012

DOI: 10.4103/0255-0857.96725

|View full text |Cite

Diagnosis of congenital toxoplasmosis by polymerase chain reaction

Sima Rasti

Mitra Behrashi

Bahram Kazemi

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2014

2016

Publication Types

Select...

Article5

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 6 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA of 0.5 g of placenta samples were extracted using DNA extraction kit, according to the manufacturer’s instructions (Bioneer, Korea). PCR was carried out to amplify the 400 bp segments of T. gondii by primer G529 with annealing temperature of 48ºC for 30 sec ( 6 ). Forward 5’-TTT TGA CTC GGG CCC AGC 3’; Reverse ; 5'-GTC CAA GCC TCC GAC TCT 3 '.…”

mentioning

confidence: 99%

“…The highest prevalence of T. gondii infection was among the age group of 20-29 years and undergraduate and housekeepers women. Based on false negativity in serological data, PCR using the placental tissue is useful for the rapid and accurate diagnosis of congenital toxoplasmosis ( 4 , 6 ). In our study, the rate of T. gondii infection using PCR and G529 primer was 3.67 times higher in the aborted placenta compared to those with normal delivery, but the difference was not significant (P = 0.17).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Molecular Diagnosis of Toxoplasma gondii in Aborted Women

Ghasemi

Rasti

Bandehpour

et al. 2014

Jundishapur J Microbiol

View full text Add to dashboard Cite

mentioning

confidence: 99%

mentioning

confidence: 99%

Molecular Diagnosis of Toxoplasma gondii in Aborted Women

Ghasemi

Rasti

Bandehpour

et al. 2014

Jundishapur J Microbiol

View full text Add to dashboard Cite

Blood Levels of Oxidant/Antioxidant Parameters in Rats Infected with Toxoplasma gondii

Bahrami

Shahriari

Tavalla

et al. 2016

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Toxoplasmosis is a common parasitic infection in the world. Since increased free radicals and oxidative stress are reported in many parasitic diseases the purpose of the present study was to evaluate the oxidative stress in acute and chronic toxoplasmosis. RH strains of Toxoplasma tachyzoites were used in the present study. Twenty-five female rats were infected with the parasite while 25 other rats were as the control group that received normal saline. Zero-, 5-, 7-, 10-, and 45-day postinfection (DPI) blood samples were taken. Some parameters related to oxidant and antioxidants such as antioxidant enzymes, malondialdehyde, and total antioxidant capacity were measured. On day 7 after infection, GPX activity and GSH level were significantly increased and in the mentioned day the amount of total antioxidant capacity was significantly reduced. In other cases, there were no significant differences between the groups in different days. Overall, based on the results it seems that, on day 7 after infection, in infected rats responses to oxidative stress were triggered and led to decrease of total antioxidant capacity. Furthermore, glutathione was increased to cope with stress. It seems that probably antioxidant defense system entered the infection to the chronic phase and changed the parasites stage.

show abstract

Molecular diagnosis of Toxoplasma gondii infection in Libya

et al. 2016

View full text Add to dashboard Cite

BackgroundToxoplasma gondii infections are prevalent in humans and animals throughout Libya. Current diagnosis is based on detection of Toxoplasma-specific IgM and IgG. In this study, we established and optimized a diagnostic PCR assay for molecular diagnosis of T. gondii in Libya.MethodsFrom January to December, 2010, 177 blood and serum samples were collected from suspected patients. This includes: 140 women who have had spontaneous abortions, 26 HIV-positive patients, nine patients with leukemia and lymphoma, and two infants with ocular infection. Samples were screened for anti-Toxoplasma IgG and IgM antibodies before DNA extraction. The surface antigen gene 2 (SAG2) was targeted in a semi-nested PCR to amplify a 999 bp and a 614 bp fragment in the first and the second run respectively.ResultsA total of 54/140 (38.5 %) women who have had spontaneous abortions, 23/26 (88 %) HIV patients, 6/9 (66.6 %) of the leukaemia and lymphoma patients, and one child with ocular infection were seropositive for anti-Toxoplasma IgG and/or IgM. Genomic DNA was extracted from 38 selected seropositive samples. The PCR was sensitive enough to detect DNA concentration of 12 ng/μL. PCR analysis was performed for 38 selected seropositive patients (16 women who have had spontaneous abortions, 15 positive HIV patients, six leukaemia patients and one child with ocular infection). Our designed primers were successfully amplified in 22/38 (57.9 %) samples; 5/12 (35.7 %) from serum and 17/26 (65.8 %) from whole blood samples. All PCR positive samples were IgG-positive except two samples which were IgM and IgG & IgM-positive serum samples respectively. The semi-nested PCR confirmed five more samples. These included two leukaemia and two HIV-positive whole blood samples and one serum sample from an aborted woman.ConclusionThe ability of PCR to diagnose active toxoplasmosis is needed in immunocompromised patients and congenital toxoplasmosis cases, especially when serological techniques fail. For the first time in Libya, we established and optimized semi-nested PCR of SAG2 gene. The developed PCR method was able to detect as little as 12 ng/μL of T. gondii DNA and was useful to diagnose the diseases in women who have had spontaneous abortions, HIV-positive patients, patients with leukemia and lymphoma, and infants with ocular infection.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Diagnosis of congenital toxoplasmosis by polymerase chain reaction

Cited by 6 publications

References 2 publications

Molecular Diagnosis of Toxoplasma gondii in Aborted Women

Molecular Diagnosis of Toxoplasma gondii in Aborted Women

Blood Levels of Oxidant/Antioxidant Parameters in Rats Infected with Toxoplasma gondii

Molecular diagnosis of Toxoplasma gondii infection in Libya

Contact Info

Product

Resources

About