Diagnosis and grading of gastritis by non-invasive optical analysis

Charvet, Igor; Ory, Gaëlle; Thueler, Philippe; Bründler, Marie-Anne; Saint-Ghislain, Michel; Azar-Pey, Nadereh; Hadengue, Antoine; Depeursinge, Christian; Vermeulen, B.; Meda, Paolo

doi:10.1097/00042737-200411000-00018

Cited by 6 publications

(6 citation statements)

References 41 publications

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“…The gastrointestinal tract, forming an ideal dark environment, can be explored by reflectance and AF analysis techniques. By spectrophotometry, Charvet et al have shown that reflectance analysis is able to better identify pathological mucosa than endoscopic examination [3]. In a previous study, we demonstrated for the first time that multispectral imaging is capable to detect reflectance modifications at specific wavelengths on visible spectrum, associated with gastric inflammation, both in a mouse model and in human [4].…”

Section: Introductionmentioning

confidence: 77%

Multimodal imaging as optical biopsy system for gastritis diagnosis in humans, and input of the mouse model

et al. 2021

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Background: Gastric inflammation is a major risk factor for gastric cancer. Current endoscopic methods are not able to efficiently detect and characterize gastric inflammation, leading to a sub-optimal patients' care. New non-invasive methods are needed. Reflectance mucosal light analysis is of particular interest in this context. The aim of our study was to analyze reflectance light and specific autofluorescence signals, both in humans and in a mouse model of gastritis. Methods: We recruited patients undergoing gastroendoscopic procedure during which reflectance was analysed with a multispectral camera. In parallel, the gastritis mouse model of Helicobacter pylori infection was used to investigate reflectance from ex vivo gastric samples using a spectrometer. In both cases, autofluorescence signals were measured using a confocal microscope. Findings: In gastritis patients, reflectance modifications were significant in near-infrared spectrum, with a decrease between 610 and 725 nm and an increase between 750 and 840 nm. Autofluorescence was also modified, showing variations around 550 nm of emission. In H. pylori infected mice developing gastric inflammatory lesions, we observed significant reflectance modifications 18 months after infection, with increased intensity between 617 and 672 nm. Autofluorescence was significantly modified after 1, 3 and 6 months around 550 and 630 nm. Both in human and in mouse, these reflectance data can be considered as biomarkers and accurately predicted inflammatory state. Interpretation: In this pilot study, using a practical measuring device, we identified in humans, modification of reflectance spectra in the visible spectrum and for the first time in near-infrared, associated with inflammatory gastric states. Furthermore, both in the mouse model and humans, we also observed modifications of autofluorescence associated with gastric inflammation. These innovative data pave the way to deeper validation studies on larger cohorts, for further development of an optical biopsy system to detect gastritis and finally to better surveil this important gastric cancer risk factor. Funding: The project was funded by the ANR EMMIE (ANR-15-CE17-0015) and the French Gastroenterology Society (SNFGE).

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Section: Introductionmentioning

confidence: 77%

Multimodal imaging as optical biopsy system for gastritis diagnosis in humans, and input of the mouse model

et al. 2021

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“…35–38 We have already shown hat the determination of these optical coefficients enable minimal alterations of skin surface to be detected in an experimental murine model, 26 and can also correctly evaluate human mucosa in patients undergoing gastroscopy for suspected gastritis alterations. 20 Here, we have tested whether the procedure can also be used to detect alterations of the vulvar epithelium, including VIN, vulvitis and lichen sclerosis. We have further assessed whether the method has a sufficient sensitivity to become a useful complement of standard clinical diagnosis, while limiting the need for an invasive biopsy for histological diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“… 17 , 19 We have found that this equipment allows for an in vivo , real-time detection of minimal tissue lesions, like those expected at the beginning of inflammatory and cancerous alterations. 15 , 20 Here we have assessed whether the method has a sufficient sensitivity to identify alterations of vulvar tissues in patients undergoing vulvoscopy. We report that the Optiprobe method discriminated normal and pathological cases, with a sensitivity and specificity that compared well with those of histological analysis, while avoiding the need of invasive biopsies.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Vulvar Lesions by Non-Invasive Optical Analysis: A Pilot Study

Vlastos¹,

Charvet²,

Dellacasa³

et al. 2009

Rare Tumors

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A procedure that could allow an early in vivo and non-invasive detection of vulvar lesions would be extremely useful. We tested an innovative optical method (Optiprobe), which uses a harmless, visible light source for the in vivo, on-line detection of minimal alterations in the structure of vulvar epithelium. A group of 3 female volunteers without gynecological symptoms were first screened to evaluate optical properties of normal vulvar tissue. Next, a group of 16 patients undergoing gynecological examination for vulvar lesions was evaluated by the Optiprobe at suspected sites before these sites were biopsied for histological analysis. Adjacent, non-involved sites were also measured to provide internal controls. Histological analysis of the biopsies identified one case that did not show obvious alterations, 4 cases of high-grade vulvar intraepithelial neoplasia (VIN), 5 cases of vulvitis, and 6 cases of lichen sclerosis (LS).The optical properties of the VIN cases were significantly different from those of controls, due to a decrease in the absorption spectra and an increase in the scattering spectra. In contrast, a significant increase in the absorption spectra and a decrease in the scattering spectra were observed in the cases of vulvitis. In the LS cases, the absorption spectra were as in controls, whereas the scattering spectra were significantly decreased. We conclude that the Optiprobe provides a useful tool for a rapid and non-invasive detection of vulvar alterations. The method should contribute to reduce the number of biopsies and to facilitate the long-term follow-up of vulvar lesions.

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“…Depending on the cellular architecture as well as the biochemical compounds embedded in the tested tissue sample, these parameters provide valuable histological information that can be effectively used for staging and grading precancerous/ cancerous tissue lesions. Such a technique, aimed at characterizing these optical properties within small tissue volumes of approximately 1 mm 3 by carrying spatially-spectrally resolved reflectance measurements, has been introduced (6,7) and successfully used through the instrument channel of an endoscope for grading gastritis (8). The development of MEMS with spectrometric capabilities would certainly benefit the development of endoscopic instruments using such non-imaging optical measurement modalities.…”

Section: Non-imaging Endoscopic Techniquesmentioning

confidence: 99%

MEMS for enhanced optical diagnostics in endoscopy

Chamot

Depeursinge

2007

Minimally Invasive Therapy & Allied Technologies

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Endoluminal microscopy and spectroscopy could significantly improve the efficiency of clinical endoscopic examination by allowing in-situ detection, staging and grading of potentially cancerous lesions. Indeed, high-end optical microscopy techniques such as confocal, coherence-gated and single-/multi-photon microscopy today deliver optical histology information and spectrally/spatially resolved measurements of tissue reflectance allow grading and staging of precancerous/cancerous lesions. Owing to the brisk development of MEMS technologies, miniaturization requirements satisfying the dimension requirements for endoscope integration have been met within the last decade and the present paper will report on the current and future development of MEMS-based endoscopes for optical diagnosis.

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Diagnosis and grading of gastritis by non-invasive optical analysis

Cited by 6 publications

References 41 publications

Multimodal imaging as optical biopsy system for gastritis diagnosis in humans, and input of the mouse model

Multimodal imaging as optical biopsy system for gastritis diagnosis in humans, and input of the mouse model

Diagnosis of Vulvar Lesions by Non-Invasive Optical Analysis: A Pilot Study

MEMS for enhanced optical diagnostics in endoscopy

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