Diabetes Promotes DMH-Induced Colorectal Cancer by Increasing the Activity of Glycolytic Enzymes in Rats

Jia, Yanglei; Xu, Gang; Wang, Zhenzheng; Meng, Linlin; Zhou, Songnan; Xu, Xia; Yuan, Huipin; Tian, Keli

doi:10.1371/journal.pone.0110455

Cited by 4 publications

(5 citation statements)

References 32 publications

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“…In addition to some well‐known pathways related to GDM, we strikingly identified the up‐regulation of several cancer‐related pathways in second‐trimester GDM samples, such as colorectal cancer, cell cycle. It has been widely reported that diabetes could result in elevated colorectal cancer risk (Jarvandi & Davidson, ; Wu et al, ), which was also confirmed in a rat model (Jia et al, ).…”

Section: Discussionmentioning

confidence: 59%

Gene coexpression network analysis identified potential biomarkers in gestational diabetes mellitus progression

Zhao

2018

Molec Gen & Gen Med

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Background Gestational diabetes mellitus (GDM) is one of the most common problems during pregnancy. Lack of international consistent diagnostic procedures has limit improvement of current therapeutic effectiveness. Here, we aimed to screen potential gene biomarkers that might play vital roles in GDM progression for assistance of its diagnostic and treatment. Methods Gene expression profiles in four GDM placentae at first trimester, four GDM placentae at second trimester, and four normal placentae were obtained from the publicly available Gene Expression Omnibus (GEO). Weighted gene coexpression network analysis (WGCNA) indicated two gene modules, that is, black and brown module, that was significantly positively and negatively correlated with GDM progression time points, respectively. Additionally, a significant positive correlation between module membership (MM) and degree in protein–protein interaction network of brown module genes was observed. Results KIF2C, CENPE, CCNA2, AURKB, MAD2L1, CCNB2, CDC20, PLK1, CCNB1, and CDK1 all have degree larger than 50 and MM larger than 0.9, so they might be valuable biomarkers in GDM. Gene set enrichment analysis inferred tight relations between carbohydrate metabolism or steroid biosynthesis‐related processes and GDM progression. Conclusions All in all, our study should provide several novel references for GDM diagnosis and therapeutic.

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Section: Discussionmentioning

confidence: 59%

Gene coexpression network analysis identified potential biomarkers in gestational diabetes mellitus progression

Zhao

2018

Molec Gen & Gen Med

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“…Previous studies have shown a close link between diabetes and colorectal cancer whereby diabetes could accelerate the incidence of colorectal cancer. The dysfunction of energy metabolism pathways has been implicated in the occurrence and development of colorectal cancer, and the abnormal expression of enzymes involved in glycometabolism might also play an important role in the development of colorectal cancer .…”

Section: Discussionmentioning

confidence: 99%

“…also described an increased incidence of colorectal cancer in a systematic review of 41 cohort studies . Previous studies have shown that the increase of glycolytic enzyme activity promotes colorectal cancer in diabetic rats . In diabetic rats, the activities of HK and PK are increased, whereas Pyruvate dehydrogenase (PDH) activity are decreased.…”

Section: Introductionmentioning

confidence: 97%

Metformin prevents DMH‐induced colorectal cancer in diabetic rats by reversing the warburg effect

Jia

Liu

et al. 2015

Cancer Medicine

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Epidemiologic studies have shown that the treatment of diabetics with metformin reduced the risk of cancer-related mortality. Here, we investigated the chemopreventive effects of metformin on dimethylhydrazine (DMH)-induced colorectal carcinogenesis in diabetic SD rats following metformin treatment and the effect on Warburg effect involved in this process. Diabetic rat models were induced with high-fat feeding in combination with a low dose of Streptozotocin (STZ) and then induce colorectal cancer with a low dose of DMH. The formation of colorectal Aberrant crypt foci (ACF) and the incidence, number and size of the tumor were measured. The proliferation indices of colonic tissues were determined through Proliferating cell nuclear antigen (PCNA) immunostaining. Then detect the expression of PK and IDH in colonic tissues using immunohistochemistry and Western blot. The enzyme activities of HK and PDH in colonic tissues were measured. The growth and expression of PK and IDH and activity of HK and PDH in cell lines LoVo and HT-29 were measured after metformin treatment. The results showed that metformin treatment significantly inhibited the formation of ACF and tumors. The proliferation index of colonic tissues was significantly decreased following metformin treatment. In addition, metformin inhibited cell growth and decreased the imbalance in the expression of the enzymes involved in glycolysis and the TCA cycle. These findings suggested that metformin might produce a synergistic colon cancer-preventative effect in diabetic patients through the regulation of the enzymes expression involved in glucose metabolism.

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“…bacteria such as lipase, amylase, and protease contributed greatly in antioxidant activity [6]. The free radicals generated in the host body cell not only degrade the cellular integrity of the cell but also damage their functionality.…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus rhamnosus ARJD as a Functional Food with Potential Antioxidant and Antibacterial Abilities

Patil¹,

Disouza²,

Pawar³

2019

Act Scie Pharma

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Lactic acid bacteria are considered as probiotics with its use as a functional food. Consumption of these article above threshold deliberates health benefits. On this basis, Lactobacillus rhamnosus ARJD (LAB) was isolated from sheep milk with its deposition as PYRN00000000-MCC 3594 in the gene bank. The isolate was screened for probiotics nature and its antioxidant properties. TheIn-vitro studies show that LAB is resistant to change in pH, bile concentration, gastric and intestinal content. Further assay like 2,2diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging, reducing activity assay and nitric oxide showed antioxidant nature of LAB. An in-vivo study carried out on Wistar rats by its liver marker investigation showed the antioxidant nature of LAB. The fecal analysis showed that LAB not only shows various probiotics properties but also thrive and get colonized inside the gastrointestinal tract. The screening of LAB against the pathogenic bacteria showed a zone of inhibition, indicating the antibacterial properties. Finally, the isolated LAB shows antibacterial and antioxidant activity conferring the health benefits.

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Diabetes Promotes DMH-Induced Colorectal Cancer by Increasing the Activity of Glycolytic Enzymes in Rats

Cited by 4 publications

References 32 publications

Gene coexpression network analysis identified potential biomarkers in gestational diabetes mellitus progression

Gene coexpression network analysis identified potential biomarkers in gestational diabetes mellitus progression

Metformin prevents DMH‐induced colorectal cancer in diabetic rats by reversing the warburg effect

Lactobacillus rhamnosus ARJD as a Functional Food with Potential Antioxidant and Antibacterial Abilities

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