Lactobacillus rhamnosus ARJD as a Functional Food with Potential Antioxidant and Antibacterial Abilities

Abstract

Lactic acid bacteria are considered as probiotics with its use as a functional food. Consumption of these article above threshold deliberates health benefits. On this basis, Lactobacillus rhamnosus ARJD (LAB) was isolated from sheep milk with its deposition as PYRN00000000-MCC 3594 in the gene bank. The isolate was screened for probiotics nature and its antioxidant properties. TheIn-vitro studies show that LAB is resistant to change in pH, bile concentration, gastric and intestinal content. Further assay like 2,2diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging, reducing activity assay and nitric oxide showed antioxidant nature of LAB. An in-vivo study carried out on Wistar rats by its liver marker investigation showed the antioxidant nature of LAB. The fecal analysis showed that LAB not only shows various probiotics properties but also thrive and get colonized inside the gastrointestinal tract. The screening of LAB against the pathogenic bacteria showed a zone of inhibition, indicating the antibacterial properties. Finally, the isolated LAB shows antibacterial and antioxidant activity conferring the health benefits.

Encapsulation of Lactic Acid Bacteria by Lyophilisation with Its Effects on Viability and Adhesion Properties

“…Similarly, Lr in all forms showed 91% ± 2.1 autoaggregation analysed by the formation of the precipitate and turbid solution formation ( Table 2 ). This similar type of work was observed in the case of Clostridium butyrium broth showing precipitate even after a wash with PBS solution [ 23 ]. The autoaggregation helps the LAB to prevent the adhesion of pathogenic microbes to gastrointestinal mucus.…”

“…Significantly, no difference in hydrophobicity was observed in the case of Lr as it showed 7.2 ± 0.51% along with its other excipient combinations ( Table 2 ). Vinderola et al tried to find such a relationship between adhesion and hydrophobicity, but none of them were successful [ 23 ]. The same results were observed in the case of both LAB, thus not showing any relationship between hydrophobicity and adhesion abilities.…”

“…Two different probiotic nature lactic acid bacteria were isolated from sheep's milk samples using selective De Man, Rogosa, and Sharpe broth and agar media (MRS). This media was gifted as a free sample from Siffin Pharma, Germany [ 19 , 22 , 23 ]. These strains were identified as Lactobacillus by morphological and molecular identification using the 16s rRNA illumination Nextseq platform technique [ 24 ].…”

Banana fibers camouflaging as a gut worm in a 6-month-old infant

“…Thus, the history of infant diet and symptoms along with the routine stool analysis is needed to be monitored thoroughly before the conclusion. Many times physicians observed to give many new probiotics formulations to infants to resolve the gastrointestinal discomfort [4][5][6][7][8][9][10].…”