Di (2-ethylhexyl) phthalate (DEHP)-induced hepatotoxicity in quails (Coturnix japonica) via triggering nuclear xenobiotic receptors and modulating cytochrome P450 systems

Zhang, Yingzhi; Zuo, Yuanmei; Du, Zheng-Hai; Xia, Jun; Zhang, Cong; Wang, Hui; Li, Xue-Nan; Li, Jinlong

doi:10.1016/j.fct.2018.07.019

Cited by 36 publications

(21 citation statements)

References 49 publications

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“…These results suggested that the expression of the Nrf2/HO‐1 pathway was inhibited by ROS generated by the DEHP treatment. Interestingly, similar mechanisms of action were observed by Zhang et al, who showed that the DEHP treatment‐induced testicular injuries in prepubertal mal Sprague‐Dawly rats through the inhibition of the Nrf2‐HO‐1 pathway. Another study from Zhao et al showed a reduction in the Nrf2 expression in testis and epididymis of mice treated with the DEHP.…”

Section: Discussionsupporting

confidence: 73%

“…People are inevitably exposed to phthalate through environmental contamination. Exposure to DEHP was associated with a variety of toxicological outcomes in mammals, reproductive toxicity, 39 neurotoxicity, 40 carcinogenicity, 41 hepatotoxicity, 42 and lipid metabolism disruption. 43 In this study, we focused on the harmful effect of this plasticizer on HEK-293 cell line, given that the kidney is one of DEHP target organ.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Di (2‐ethylhexyl) phthalate induces cytotoxicity in HEK‐293 cell line, implication of the Nrf‐2/HO‐1 antioxidant pathway

Amara

Timoumi

Graiet

et al. 2019

Environmental Toxicology

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The di (2‐ethylhexyl) phthalate (DEHP) is a plasticizer used in the polyvinyl chloride industry. Human exposure to this plasticizer is inevitable and contributes to several side effects. In this study, we examined whether DEHP induces apoptosis and oxidative stress in embryonic kidney cells (HEK‐293) and whether the nuclear factor E2‐related factor 2 (Nrf‐2)/heme oxygenase‐1 (HO‐1) antioxidant pathway is involved in the pathogenesis of this process. We demonstrated that DEHP is cytotoxic to HEK‐293 cells. It causes oxidative damage through the generation of free radicals, induces lipid peroxidation, and alters superoxide dismutase and catalase activities. Simultaneously, DEHP treatment decreases the expression and the protein level of Nrf‐2 and HO‐1. Inhibition of the Nrf‐2/HO‐1 pathway is related to the mitochondrial pathway of apoptosis. This apoptotic process is characterized by a loss of mitochondrial transmembrane potential (ΔΨm) and upregulation of the expression of caspase‐3 mRNA as well as its protein level.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

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Di (2‐ethylhexyl) phthalate induces cytotoxicity in HEK‐293 cell line, implication of the Nrf‐2/HO‐1 antioxidant pathway

Amara

Timoumi

Graiet

et al. 2019

Environmental Toxicology

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“…Many PAEs are demonstrated to be toxic to reproduction, impair development in aquatic animals (amphibians and crustaceans), and induce genetic mutation . DEHP can cause hepatotoxicity in quails via triggering nuclear xenobiotic receptors and modulating cytochrome P450 systems (Zhang Y.-Z. et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Photodegradation Pathways of Typical Phthalic Acid Esters Under UV, UV/TiO2, and UV-Vis/Bi2WO6 Systems

Wang

Zeng

et al. 2019

Front. Chem.

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Photolysis and photocatalysis of typical phthalic acid esters (dimethyl phthalate, DMP; diethyl phthalate, DEP; dibutyl phthalate, DBP) were carried out in UV, UV/TiO 2 , and UV-Vis/Bi 2 WO 6 systems. All of the selected phthalic acid esters and their decomposition byproducts were subjected to qualitative and quantitative analysis through HPLC and GC-MS. The results of 300 min of photolysis and photodegradation reaction were that each system demonstrated different abilities to remove DMP, DEP, and DBP. The UV/TiO 2 system showed the strongest degradation ability on selected PAEs, with removal efficiencies of up to 93.03, 92.64, and 92.50% for DMP, DEP, and DBP in 90 min, respectively. UV-Vis/Bi 2 WO 6 had almost no ability to remove DMP and DEP. However, all of the systems had strong ability to degrade DBP. On the other hand, the different systems resulted in various byproducts and PAE degradation pathways. The UV system mainly attacked the carbon branch and produced o-hydroxybenzoates. No ring-opening byproducts were detected in the UV system. In the photocatalytic process, the hydroxyl radicals produced not only attacked the carbon branch but also the benzene ring. Therefore, hydroxylated compounds and ring-opening byproducts were detected by GC-MS in both the UV/TiO 2 and UV-Vis/Bi 2 WO 6 photocatalytic systems. However, there were fewer products due to direct hole oxidation in the UV-Vis/Bi 2 WO 6 system compared with the UV/TiO 2 system, which mainly reacted with the pollutants via hydroxyl radicals.

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“…As the most widely used PAEs, Di-(2-ethylhexyl) phthalate (DEHP) has been detected around the world in recent years [14]. Many studies have proved that DEHP has strong toxicity, including carcinogenicity, teratogenicity, reproductive toxicity, hepatotoxicity, mutagenicity and endocrine disruption toxicity [15,16]. In Japan, DEHP has caused 13.7 million disability-adjusted life years (DALYs) to humans [17].…”

Section: Introductionmentioning

confidence: 99%

Biochemical Pathways and Enhanced Degradation of Dioctyl Phthalate (DEHP) by Sodium Alginate Immobilization in MBR System

Zhang

Luo

et al. 2020

Preprint

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A bacterial strain that could effectively degrade DEHP was isolated from the activated sludge and identified as Bacillus sp. by DNA sequencing. The biochemical degradation pathway of DEHP was further analyzed by GC-MS, and the results showed that DEHP was first decomposed into phthalates (DBP). Diuretic sylycol (DEP) was then generated, and phthalates (PA) were generated by a continuous de-ehelateization reaction. Phthalic acid (PA) was oxidized, dehydrogenated, and decarboxylated into protocatechins. Protocatechins enter the TCA cycle through orthotopic ring opening.　To enhance DEHP degradation, sodium alginate and calcium chloride were used as embedding and cross-linking materials, and the strain was immobilized. The immobilization conditions were optimized via an orthogonal experiment, and the results showed that the optimal immobilization conditions were SA mass fraction of 4%, CaCl2 mass fraction of 5%, ratio of bacteria to SA of 1:1, and the crosslinking time of 6 hours. The immobilized bacteria agent was further applied to MBR systems. The results showed that the removal rate of DEHP (5mg/L) in the system by immobilized bacteria was 91.9%, which is significantly higher than that of free bacteria. The 3, 4-dioxygenase gene and microbial community dynamics were analyzed by q-PCR and Illumina Miseq sequencing. The q-PCR results showed that the number of copies of 3, 4-dioxygenase gene in the immobilized system was significantly higher than that of free bacteria. Illumina Miseq sequencing results showed that Micromonospora, Rhodococcus, Bacteroides and Pseudomonas were the dominant generas in the MBR system. The analysis of bacterial community structure indicated that immobilization technology had a positive impact on the system stability. The results implied that this immobilized technique had potential applications in DEHP wastewater treatment.

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Di (2-ethylhexyl) phthalate (DEHP)-induced hepatotoxicity in quails (Coturnix japonica) via triggering nuclear xenobiotic receptors and modulating cytochrome P450 systems

Cited by 36 publications

References 49 publications

Di (2‐ethylhexyl) phthalate induces cytotoxicity in HEK‐293 cell line, implication of the Nrf‐2/HO‐1 antioxidant pathway

Di (2‐ethylhexyl) phthalate induces cytotoxicity in HEK‐293 cell line, implication of the Nrf‐2/HO‐1 antioxidant pathway

Photodegradation Pathways of Typical Phthalic Acid Esters Under UV, UV/TiO2, and UV-Vis/Bi2WO6 Systems

Biochemical Pathways and Enhanced Degradation of Dioctyl Phthalate (DEHP) by Sodium Alginate Immobilization in MBR System

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