Di-2-ethylhexyl phthalate (DEHP) exposure induces sperm quality and functional defects in mice

Liu, Xuexia; Liu, YaNan; Zi, Teng; YuSheng, Zhang; Wang, Zelin; Peng, Zhu; Xie, Meng; Liu, Fujun

doi:10.1016/j.chemosphere.2022.137216

Cited by 10 publications

(5 citation statements)

References 34 publications

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“…The occurrence of spermatozoa malformations (shortening of body length, pericardial cyst, yolk cyst and tail askew) showed a concentration-dependent increase following TBPH exposure. Similarly, DEHP has also been reported to induce sperm quality and functional defects in mice . Therefore, our results confirmed that subchronic exposure to TBPH at environmentally relevant doses could affect the sperm traits (morphology and motility) in male zebrafish, which may contribute to the above-mentioned abnormal development of their offspring.…”

Section: Results and Discussionsupporting

confidence: 86%

“…Similarly, DEHP has also been reported to induce sperm quality and functional defects in mice. 57 Therefore, our results confirmed that subchronic exposure to TBPH at environmentally relevant doses could affect the sperm traits (morphology and motility) in male zebrafish, which may contribute to the above-mentioned abnormal development of their offspring.…”

Section: Impairment On Sperm Motility and Morphologysupporting

confidence: 80%

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Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Fu,

Hua,

Zhang

et al. 2023

Environ. Sci. Technol.

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Bis(2-ethylhexyl)tetrabromophthalate (TBPH) has been widely detected in the environment and organisms; thus, its toxic effects on male reproduction were systematically studied. First, we found that TBPH can stably bind to the androgen receptor (AR) based on in silico molecular docking results and observed an antagonistic activity, but not agonistic activity, on the AR signaling pathway using a constructed AR-GRIP1 yeast assay. Subsequently, we validated the adverse effects on male germ cells by observing inhibited androgen production and proliferation in Leydig cells upon in vitro exposure and affected general motility and motive tracks of zebrafish sperm upon ex vivo exposure. Finally, the in vivo reproductive toxicity was demonstrated in male zebrafish by reduced mating behavior in F0 generation when paired with unexposed females and abnormal development of their offspring. In addition, reduced sperm motility and impaired germ cells in male zebrafish were also observed, which may be related to the disturbed homeostasis of sex hormones. Notably, the specifically suppressed AR in the brain provides further evidence for the antagonistic effects as above-mentioned. These results confirmed that TBPH affected male reproduction through a classical nuclear receptor-mediated pathway, which would be helpful for assessing the ecological and health risks of TBPH.

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Section: Results and Discussionsupporting

confidence: 86%

Section: Impairment On Sperm Motility and Morphologysupporting

confidence: 80%

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Fu,

Hua,

Zhang

et al. 2023

Environ. Sci. Technol.

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“…Previous studies have reported that exposure to pollutants could affect the sperm quality and reproductive success in zebrafish or in mice. 31,32 In contrast, we also observed significantly increased average pathway velocity (Figure 2C), straight-line velocity (Figure 2D) as well as curvilinear velocity (Figure 2E), which indicated hyperactivity of sperm from the males at 0.1 μg/L BTBPE treatment. Previous studies have proved that exposure to decabromodiphenyl ethane (DBDPE), another NBFR, at lower concentrations may evoke an excitatory response before causing toxic effects in zebrafish.…”

Section: Btbpe Impaired the Reproductive Health Status Of Male Zebrafishmentioning

confidence: 79%

Mechanistic Insights into 1,2-bis(2,4,6-tribromophenoxy)ethane-Induced Male Reproductive Toxicity in Zebrafish

Zheng,

Wang,

Zhang

et al. 2024

Environ. Sci. Technol.

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The novel brominated flame retardant, 1,2-bis(2,4,6tribromophenoxy)ethane (BTBPE), has increasingly been detected in environmental and biota samples. However, limited information is available regarding its toxicity, especially at environmentally relevant concentrations. In the present study, adult male zebrafish were exposed to varying concentrations of BTBPE (0, 0.01, 0.1, 1, and 10 μg/L) for 28 days. The results demonstrated underperformance in mating behavior and reproductive success of male zebrafish when paired with unexposed females. Additionally, a decline in sperm quality was confirmed in BTBPE-exposed male zebrafish, characterized by decreased total motility, decreased progressive motility, and increased morphological malformations. To elucidate the underlying mechanism, an integrated proteomic and phosphoproteomic analysis was performed, revealing a predominant impact on mitochondrial functions at the protein level and a universal response across different cellular compartments at the phosphorylation level. Ultrastructural damage, increased expression of apoptosis-inducing factor, and disordered respiratory chain confirmed the involvement of mitochondrial impairment in zebrafish testes. These findings not only provide valuable insights for future evaluations of the potential risks posed by BTBPE and similar chemicals but also underscore the need for further research into the impact of mitochondrial dysfunction on reproductive health.

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“…Previous studies[ 23 , 24 ] have reported that DEHP is soluble in corn oil. Hence, this study used corn oil as the solvent for DEHP.…”

Section: Methodsmentioning

confidence: 99%

Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice

Shi,

Zhao,

Peng

et al. 2023

World J Gastroenterol

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Esophageal cancer (EC) ranks among the most prevalent malignant tumors affecting the digestive tract. Esophageal squamous cell carcinoma (ESCC) stands as the prevailing pathological subtype, encompassing approximately 90% of all EC patients. In clinical stage II-IVA locally advanced ESCC cases, the primary approach to treatment involves a combination of neoadjuvant therapy and surgical resection. Despite concerted efforts, the long-term outcomes for ESCC patients remain unsatisfactory, with dismal prognoses. However, recent years have witnessed remarkable strides in immunotherapy, particularly in the second- and first-line treatment of advanced or metastatic ESCC, with the development of monoclonal antibodies that inhibit programmed death 1 or programmed death ligand 1 demonstrating encouraging responses and perioperative clinical benefits for various malignancies, including ESCC. This comprehensive review aims to present the current landscape of perioperative immunotherapy for resectable ESCC, focusing specifically on the role of immune checkpoint inhibitors during the perioperative period. Additionally, the review will explore promising biomarkers and offer insights into future prospects.

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Di-2-ethylhexyl phthalate (DEHP) exposure induces sperm quality and functional defects in mice

Cited by 10 publications

References 34 publications

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Integrated Studies on Male Reproductive Toxicity of Bis(2-ethylhexyl)-tetrabromophthalate: in Silico, in Vitro, ex Vivo, and in Vivo

Mechanistic Insights into 1,2-bis(2,4,6-tribromophenoxy)ethane-Induced Male Reproductive Toxicity in Zebrafish

Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice

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