Di 2-ethyl hexyl phthalate affects differentiation and matrix mineralization of rat calvarial osteoblasts – in vitro

Bhat, Firdous Ahmad; Govindan, Ramajayam; Parameswari, S.; Vignesh, R.; Kalimuthu, S; Karthikeyan, G.; Karthikeyan, S.; Arunakaran, J.; Srinivasan, N.

doi:10.1016/j.tiv.2012.09.003

Cited by 33 publications

(17 citation statements)

References 43 publications

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“…In this respect, it has been demonstrated that an in vitro BBP treatment (10 −6 M) for 6 hr in rat osteoblastic cultures induced increased D3 expression and abnormal cell proliferation via the agonistic effects of 17‐β‐estradiol (Agas et al, ). The above data are in agreement with other findings, which showed that the DEHP treatment (10 M) for 48 hr in rat calvarial cultures augmented cell proliferation and decreased differentiated markers such as ALP, TAZ, Runx2 and collagen synthesis (Bhat et al, ).…”

Section: Bone and Bone Marrow‐disrupting Chemicalssupporting

confidence: 93%

Bone and bone marrow disruption by endocrine‐active substances

Agas

Lacava

Sabbieti

2018

Journal Cellular Physiology

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Bone is a multifaceted dynamic tissue, involved in mobility, mineral metabolism, and mesenchymal or stromal and hematopoietic progenitor or stem cells breading. Recently, an endocrine role has been attributed to bone due to its ability to produce at least two hormones (osteocalcin and fibroblast growth factor 23) and to participate directly or indirectly in leptin, insulin, estrogens, and serotonin signaling; regulation; and action. Also, bearing in mind the enormous amounts of substances secreted by the different bone marrow cell types, it becomes understandable the contribution of bone tissue to systemic homeostasis. Besides, bone is a well-known estrogen-responsive tissue, reacting to environmental influences. Thus, it has been coined as a critical target of environmental xenoestrogens, known as endocrine-disrupting chemicals (EDCs). The exposure to EDCs results to disruption or imbalance of the systemic hormonal regulation of the skeleton including bone modeling and remodeling, local hormones, and cytokine or chemokine release. The present report highlights the harmful EDCs effects on bone tissue and provides up-to-date information of xenoestrogen action on proliferation, maturation, and homing of bone marrow inhabitants.

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Section: Bone and Bone Marrow‐disrupting Chemicalssupporting

confidence: 93%

Bone and bone marrow disruption by endocrine‐active substances

Agas

Lacava

Sabbieti

2018

Journal Cellular Physiology

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“…ROB were isolated from neonatal (<3 day old) Sprague-Dawley rat calvarias by a sequential enzymatic digestion method according to Bhat et al [18] and Hino et al [19] with minor modifications. Briefly, eight calvariae were excised aseptically, cleaned of soft tissues, and cut into fragments (1 mm × 1 mm × 1 mm).…”

Section: Methodsmentioning

confidence: 99%

“…After incubation for 4 h, formazan crystals formed were dissolved in DMSO and the absorbance value was determined at 490 nm immediately. The effects of treatments on cell viability were expressed as ratio of Absorbance of treated cells/Absorbance of control cells [18]. …”

Section: Methodsmentioning

confidence: 99%

The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone ResorptionIn Vitro

Zhai

Pan

Niu

et al. 2014

International Journal of Endocrinology

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Osteoporosis treatment always aimed at keeping the balance of bone formation and bone resorption. Recently, prenyl group in natural products has been proposed as an active group to enhance the osteogenesis process. Osthole has both the prenyl group and bone-protective activities, but the relationship is still unknown. In this study we found that osthole exerted a potent ability to promote proliferation and osteogenic function of rat bone marrow stromal cells and osteoblasts, including improved cell viability, alkaline phosphatase activity, enhanced secretion of collagen-I, bone morphogenetic protein-2, osteocalcin and osteopontin, stimulated mRNA expression of insulin-like growth factor-1, runt-related transcription factor-2, osterix, OPG (osteoprotegerin), RANKL (receptor activator for nuclear factor-κB ligand), and the ratio of OPG/RANKL, as well as increasing the formation of mineralized nodules. However, 7-methoxycoumarin had no obvious effects. Osthole also inhibited osteoclastic bone resorption to a greater extent than 7-methoxycoumarin, as shown by a lower tartrate-resistant acid phosphatase activity and lower number and smaller area of resorption pits. Our findings demonstrate that osthole could be a potential agent to stimulate bone formation and inhibit bone resorption, and the prenyl group plays an important role in these bone-protective effects.

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“…MEHP has been shown to significantly suppress ALP activity, Runx2 expression, and osterix expression in MSCs (76). DEHP results in reduction of ALP expression, Runx2 protein levels, and mineralization in osteoblasts (105). The mechanisms by which 10 µM of MEHP and DEHP and 20 µM of MEHP inhibited osteogenesis were not determined in these studies, although Watt and Schlezinger and Bhat et al demonstrated that the reduction in osteogenesis was not a result of decreased cell viability.…”

Section: Osteogenesismentioning

confidence: 99%

The Effects of Endocrine Disruptors on Adipogenesis and Osteogenesis in Mesenchymal Stem Cells: A Review

et al. 2017

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Endocrine-disrupting chemicals (EDCs) are prevalent in the environment, and epidemiologic studies have suggested that human exposure is linked to chronic diseases, such as obesity and diabetes. In vitro experiments have further demonstrated that EDCs promote changes in mesenchymal stem cells (MSCs), leading to increases in adipogenic differentiation, decreases in osteogenic differentiation, activation of pro-inflammatory cytokines, increases in oxidative stress, and epigenetic changes. Studies have also shown alteration in trophic factor production, differentiation ability, and immunomodulatory capacity of MSCs, which have significant implications to the current studies exploring MSCs for tissue engineering and regenerative medicine applications and the treatment of inflammatory conditions. Thus, the consideration of the effects of EDCs on MSCs is vital when determining potential therapeutic uses of MSCs, as increased exposure to EDCs may cause MSCs to be less effective therapeutically. This review focuses on the adipogenic and osteogenic differentiation effects of EDCs as these are most relevant to the therapeutic uses of MSCs in tissue engineering, regenerative medicine, and inflammatory conditions. This review will highlight the effects of EDCs, including organophosphates, plasticizers, industrial surfactants, coolants, and lubricants, on MSC biology.

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Di 2-ethyl hexyl phthalate affects differentiation and matrix mineralization of rat calvarial osteoblasts – in vitro

Cited by 33 publications

References 43 publications

Bone and bone marrow disruption by endocrine‐active substances

Bone and bone marrow disruption by endocrine‐active substances

The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone ResorptionIn Vitro

The Effects of Endocrine Disruptors on Adipogenesis and Osteogenesis in Mesenchymal Stem Cells: A Review

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