DHI Increases the Proliferation and Migration of Schwann Cells Through the PI3K/AKT Pathway and the Expression of CXCL12 and GDNF to Promote Facial Nerve Function Repair

Abstract

BackgroundDanhong injection (DHI) is a commonly used drug in the treatment of cardiovascular and cerebrovascular diseases, and its neuroprotective research has been fully con rmed. Schwann cells, as myelin forming cells of peripheral nerve, play an important role in the process of injury and repair. The purpose of this study was to explore the effect of DHI on Schwann cells and its role in facial nerve injury. MethodsRSC 96 Schwann cells were treated with different concentrations (0 -2%) of DHI for different time intervals (12 and 36 h). Effect of DHI on cell viability and migration were determined by CCK8 and Transwell assays. The levels of PI3K-Akt signaling related proteins were measured by western blotting analysis, and the effects of DHI on GDNF and CXCL12 using Western Blot, RT-qPCR, and ELISA assays at the cellular and animal levels, respectively. Then LY294002, an inhibitor of PI3K, was used to study the

Electrospun Composite PLLA‐PPSB Nanofiber Nerve Conduits for Peripheral Nerve Defects Repair and Regeneration

“…Gao et al found that activating the PI3K/Akt pathway boosted SC proliferation, aiding remyelination and nerve regeneration post-facial nerve injury. [70] Ma et al found that the proliferative capability of SCs was restored by downregulating the long non-coding RNA, which negatively affected the PI3K/Akt pathway, subsequently facilitating functional recovery in rats post sciatic nerve injury. [71] Therefore, it can be inferred that PPSB 60:40 nanofibers enhanced SC proliferation by activating the PI3K/Akt signaling pathway.…”

Neural stem cell transplantation improves neurological function in focal cerebral ischemia through GDNF/PI3K/AKT axis

“…The endogenous nervous system consists of intestinal neurons and intestinal glial cells, of which the latter can maintain the integrity of the enteric nervous system and secrete glial cell-derived neurotrophic factor (GDNF), a nutritional factor that promotes neuronal growth, differentiation, and repair (6). The PI3K/AKT axis involved in GDNF has also been shown to work in the maintenance and differentiation of NSCs in the developing brain and contributes to the recovery of neurological function after CI injury (7,8). However, it remains to characterize whether NSC transplantation plays a role in treating ischemic brain injury through the GDNF/PI3K/AKT axis.…”