DHEA improves the antioxidant capacity of endometrial stromal cells and improves endometrium receptivity via androgen receptor

Qin, Aiping; Qin, Junqi; Jin, Yufu; Li, Fan; Jiang, Lin; Mo, Fuhua

doi:10.1016/j.ejogrb.2016.01.016

Cited by 22 publications

(19 citation statements)

References 28 publications

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“…Therefore, a third possibility of augmented HOXA-10 expression after DHEA might be related to an AR enhancer effect of DHEA. The greatest support for our hypothesis come from the study by Qin et al ( 39 ) who investigated the impact of DHEA on decidual PRL-related protein (dPRP), AR, and HOXA-10 expressions in mouse endometrial cells. They reported that DHEA had an insignificant effect on endometrial dPRP expression.…”

Section: Discussionmentioning

confidence: 62%

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DHEA supplementation improves endometrial HOXA-10 mRNA expression in poor responders

Çelik

Acet

Imren

et al. 2017

J Turkish German Gynecol Assoc

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Objective:The study was planned to investigate whether DHEA supplementation had an impact on endometrial receptivity in women who were poor responders (POR).Material and Methods:Twenty-eight POR women who were undergoing hysteroscopy and five fertile control subjects were included. The POR women were equally subdivided into two separate groups as patients who were currently using DHEA and those who were not. Endometrial samples of the subjects were obtained during hysteroscopy at the late follicular phase. Expression levels of endometrial HOXA-10, HOXA-11, and LIF mRNA were measured with the using real-time polymerase chain reaction. Spontaneous clinical pregnancy rates were also noted.Results:Compared with POR women who were not given DHEA, upregulated endometrial HOXA-10 (7.33-fold) and HOXA-11 (2.39-fold) mRNA expression were detected in POR women on DHEA. The increase in HOXA-10 mRNA was significant (p<0.03). The fold increase in HOXA-11 mRNA was found as 2.39, which indicated a positive upregulation. However, this fold increment was insignificant (p<0.45). An insignificant increase in spontaneous clinical pregnancy rates in POR women on DHEA (53.3%) was observed compared with POR women who were not given DHEA (43.8%).Conclusion:Oral DHEA supplementation in POR upregulates endometrial HOXA-10 mRNA expression, which is known to positively modulate endometrial receptivity.

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Section: Discussionmentioning

confidence: 62%

“…Moreover, they showed that DHEA-mediated upregulation in HOXA-10 was diminished by treatment with an AR antagonist. Together, if physiologic androgen deficiency in aging women leads to a decline in AR, we can suggest that exogenous DHEA can increase endometrial AR, which might lead to a rise in HOXA-10 ( 39 ).…”

Section: Discussionmentioning

confidence: 95%

DHEA supplementation improves endometrial HOXA-10 mRNA expression in poor responders

Çelik

Acet

Imren

et al. 2017

J Turkish German Gynecol Assoc

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“…Antioxidant and cytoprotective effects of BCB extract ROS, oxidative stress, and antioxidants have been implicated in the establishment and progression of pregnancy including embryo implantation, placental differentiation, and embryo growth [21,22]. Recently, a study reported that dehydroepiandrosterone improves endometrium receptivity and enhances embryo implantation by inhibiting the generation of intracellular ROS in endometrial stromal cells [23]. On that basis, we hypothesized that the bene cial effects of BCB are probably due to its antioxidant effect.…”

Section: Resultsmentioning

confidence: 97%

“…ROS concentration may also have major effects on the implantation and fertilization of eggs [72]. Recently, Qin et al (2016) reported that dehydroepiandrosterone improves endometrium receptivity and enhance embryo implantation by inhibiting the generation of intracellular ROS in endometrial stromal cells [23], and Nicol et al (2000) reported glucose 6-phosphate dehydrogenase prevented oxidative stress-induced embryopathies [73]. In concert with those ndings, in this study, BCB showed strong scavenging activities against DPPH and the superoxide anion radicals, and inhibited ROS production in CHO-K1 cells treated with H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

Administration of Herb Formulation Enhanced Blastocyst Implantation via Iκb Activation in Mouse Endometrium

Jeon

Liu

Cai

et al. 2020

Preprint

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BackgroundBaelan Chagsang Bang (BCB), consisting of a mixture of 11 herbs, may be prescribed as a reproductive function supplement to improve ovulation and implantation during the treatment of infertility and recurrent abortion in Oriental Medicine. This study aimed to investigate the effects and mechanisms of action of water-extracted BCB on endometrial receptivity and blastocyst implantation in a mifepristone (RU486)-induced implantation failure model.MethodsIn in vitro, antioxidant potentials of BCB were evaluated using DPPH and superoxide anion radical scavenging assays and DCFH-DA assay. In vivo, C57BL/6 female mice (n = 6 per group) orally received BCB (300 mg/kg/day), a dose similar to that used clinically. BCB was administrated from 7 days before pregnancy until the end of the experiment. They were housed with male mice until pregnancy was confirmed. On day 4 of pregnancy, RU486 (4 mg/kg) was injected subcutaneously to induce implantation failure.ResultsAdministration of the BCB water extract increased the number of newborn pups in BCB-treated mice compared to that of sham-treated mice and improved the number of implantation sites in pregnant mice despite RU486 injection. BCB increased the protein expression level of cyclooxygenase-2 and inducible nitric oxide synthase through IκB activation. Moreover, the expression level of matrix metalloproteinases (MMPs) at the uterus implantation site was up-regulated in the BCB-treated group compared to that of the RU486-treated group. To determine the mechanisms of action of BCB, the anti-oxidative effect of BCB was examined via in vitro assay. The BCB water extract showed strong anti-oxidative effects in the DPPH assay and the superoxide anion free-radical scavenging activity assay.ConclusionOur findings provide evidence that BCB has therapeutic potential against poor endometrial receptivity.

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“…Excess ROS in follicles may overwhelm follicular uid antioxidant defense, directly damage oocytes, cause defective fertilization, and even when fertilization has been achieved, oxidative stress-induced apoptosis may result in embryo fragmentation, implantation failure, abortion, impaired placentation, or congenital abnormalities [56]. Nicol et al (2000) reported glucose 6-phosphate dehydrogenase prevented oxidative stress-induced embryopathies [57], and more recently, Qin et al (2016) reported that dehydroepiandrosterone improves endometrium receptivity and enhances embryo implantation by inhibiting the generation of intracellular ROS in endometrial stromal cells [58]. In concert with these ndings, in the present study, BCB showed strong scavenging activities against DPPH and superoxide anion radicals and inhibited ROS production in CHO-K1 cells treated with H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

Administration of a herbal formulation enhanced blastocyst implantation via IκB activation in mouse endometrium

Jeon

Liu

Cai

et al. 2020

Preprint

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Background: BaelanChagsangBang (BCB), a herbal formulation consisting of eleven herbs, may be prescribed as a reproductive functional supplement to improve ovulation and implantation during the treatment of infertility and recurrent abortion in Korean Medicine. This study aimed to investigate the effects and action mechanisms of water-extracted BCB on endometrial receptivity and blastocyst implantation under normal conditions and in a mifepristone (RU486)-induced implantation failure murine model.Methods: In vitro, the antioxidant potentials of BCB were evaluated using DPPH and superoxide anion radical scavenging assays and a DCFH-DA assay, and the cytotoxic and cytoprotective effects of BCB were confirmed using an MTT assay. In vivo, C57BL/6 female mice (n = 6 per group) orally received BCB (300 mg/kg/day), a dose similar to that used clinically, from 7 days before pregnancy until the end of the experiment. On day 4 of pregnancy, RU486 (4 mg/kg) was injected subcutaneously to induce implantation failure. The effect of BCB on embryo implantation was evaluated by implantation rate analysis, histological examination, and western blotting of uterus tissues.Results: BCB water extract showed strong anti-oxidative and cytoprotective effects in vitro. In vivo administration of BCB water extract increased the number of newborn pups in BCB-treated mice versus sham-treated mice under normal conditions and improved the number of implantation sites in pregnant mice despite RU486 injection. BCB increased the protein levels of cyclooxygenase-2 and inducible nitric oxide synthase through IκB activation. Moreover, the expression levels of matrix metalloproteinases (MMPs) at uterus implantation sites were up-regulated in the BCB-treated group as compared with those in the RU486-treated group. Conclusion: These results show BCB improved embryo implantation through IκB activation in our mouse model and suggest that BCB has therapeutic potential in the context of poor endometrial receptivity.

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DHEA improves the antioxidant capacity of endometrial stromal cells and improves endometrium receptivity via androgen receptor

Cited by 22 publications

References 28 publications

DHEA supplementation improves endometrial HOXA-10 mRNA expression in poor responders

DHEA supplementation improves endometrial HOXA-10 mRNA expression in poor responders

Administration of Herb Formulation Enhanced Blastocyst Implantation via Iκb Activation in Mouse Endometrium

Administration of a herbal formulation enhanced blastocyst implantation via IκB activation in mouse endometrium

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