DgC3H1, a CCCH zinc finger protein gene, confers cold tolerance in transgenic chrysanthemum

Bai, Huiru; Lin, Ping; Li, Xin; Liao, Xiaoqin; Wan, Lihua; Yang, Xiaohan; Luo, Yunchen; Zhang, Lei; Fan, Zhengqiu; Liu, Shiliang; Liu, Qinglin

doi:10.1016/j.scienta.2021.109901

Cited by 16 publications

(15 citation statements)

References 29 publications

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“…Several MBS cis -acting elements associated with drought inducibility within the promoter regions of HvC3H28 and HvC3H1 were predicted, suggesting that these genes might play a potential role in the response to drought stress. In Chrysanthemum , DgC3H1 enhances low-temperature tolerance by regulating the ROS system and the expression of downstream cold-related genes [ 24 ]. However, the expression of HvC3H43 , which is orthologous to DgC3H1 , was not induced by cold stress according to RNA-seq and qRT-PCR analyses.…”

Section: Discussionmentioning

confidence: 99%

“…Switchgrass PvC3H72 was the first CCCH family gene identified to be involved in plant chilling and freezing tolerance, possibly through an ABA-mediated pathway [ 23 ]. Moreover, DgC3H1 confers cold tolerance in Chrysanthemum plants by regulating the osmotic and reactive oxygen species (ROS) system, as well as the expression of genes associated with the cold stress response [ 24 ]. In addition, CCCH genes are involved in other adaptive processes, such as resistance to bacterial blight disease [ 25 ], zinc homeostasis [ 26 ], hydrogen peroxide [ 11 ], and oxidative stress [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Pan

Zeng

et al. 2022

BMC Plant Biol

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Background CCCH transcription factors are important zinc finger transcription factors involved in the response to biotic and abiotic stress and physiological and developmental processes. Barley (Hordeum vulgare) is an agriculturally important cereal crop with multiple uses, such as brewing production, animal feed, and human food. The identification and assessment of new functional genes are important for the molecular breeding of barley. Results In this study, a total of 53 protein-encoding CCCH genes unevenly dispersed on seven different chromosomes were identified in barley. Phylogenetic analysis categorized the barley CCCH genes (HvC3Hs) into eleven subfamilies according to their distinct features, and this classification was supported by intron–exon structure and conserved motif analysis. Both segmental and tandem duplication contributed to the expansion of CCCH gene family in barley. Genetic variation of HvC3Hs was characterized using publicly available exome-capture sequencing datasets. Clear genetic divergence was observed between wild and landrace barley populations in HvC3H genes. For most HvC3Hs, nucleotide diversity and the number of haplotype polymorphisms decreased during barley domestication. Furthermore, the HvC3H genes displayed distinct expression profiles for different developmental processes and in response to various types of stresses. The HvC3H1, HvC3H2 and HvC3H13 of arginine-rich tandem CCCH zinc finger (RR-TZF) genes were significantly induced by multiple types of abiotic stress and/or phytohormone treatment, which might make them as excellent targets for the molecular breeding of barley. Conclusions Overall, our study provides a comprehensive characterization of barley CCCH transcription factors, their diversity, and their biological functions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Pan

Zeng

et al. 2022

BMC Plant Biol

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“…In addition, compared with the wild type, low temperature stress-related genes such as DgCOR413 , DgDREBa , DgCSD1 and DgCSD2 were up-regulated in the overexpression line and down-regulated in the antisense line. The results showed that DgC3H1 can act as a transcription factor to improve the resistance of Chrysanthemum to cold stress [ 191 ].…”

Section: The Roles Of Ccch Zinc-finger Proteins In Abiotic Stressmentioning

confidence: 99%

The Roles of CCCH Zinc-Finger Proteins in Plant Abiotic Stress Tolerance

Han

Qiao

et al. 2021

IJMS

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Zinc-finger proteins, a superfamily of proteins with a typical structural domain that coordinates a zinc ion and binds nucleic acids, participate in the regulation of growth, development, and stress adaptation in plants. Most zinc fingers are C2H2-type or CCCC-type, named after the configuration of cysteine (C) and histidine (H); the less-common CCCH zinc-finger proteins are important in the regulation of plant stress responses. In this review, we introduce the domain structures, classification, and subcellular localization of CCCH zinc-finger proteins in plants and discuss their functions in transcriptional and post-transcriptional regulation via interactions with DNA, RNA, and other proteins. We describe the functions of CCCH zinc-finger proteins in plant development and tolerance to abiotic stresses such as salt, drought, flooding, cold temperatures and oxidative stress. Finally, we summarize the signal transduction pathways and regulatory networks of CCCH zinc-finger proteins in their responses to abiotic stress. CCCH zinc-finger proteins regulate the adaptation of plants to abiotic stress in various ways, but the specific molecular mechanisms need to be further explored, along with other mechanisms such as cytoplasm-to-nucleus shuttling and post-transcriptional regulation. Unraveling the molecular mechanisms by which CCCH zinc-finger proteins improve stress tolerance will facilitate the breeding and genetic engineering of crops with improved traits.

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“…However, the expression of HvC3H27, which is orthologous to DgC3H1, was not induced by cold stress for both RNA-seq and qRT-PCR analysis. Moreover, there was no cis-acting element, such as LTR, involved in low temperature responsiveness within the promoter region of HvC3H27 [31]. These results indicate that HvC3H27 might have functionally diversi ed in barley.…”

Section: Expansion Patterns Might Affect the Gene Family Size Of Hvc3hsmentioning

confidence: 86%

“…Overexpression of PvC3H72 improves cold tolerance in switchgrass, possibly via the ABA-mediated pathway [30]. More recently, DgC3H1 has been shown to confer cold tolerance in Chrysanthemum plants by regulating the osmotic and ROS system, as well as the expression of genes associated with the response to cold stress [31]. In addition, CCCH proteins have been shown to be involved in other adaptive processes, such as bacterial blight disease resistance [32], zinc homeostasis [33], and hydrogen peroxide [17] and oxidative stress [34].…”

Section: Introductionmentioning

confidence: 99%

CCCH Zinc Finger Genes in Barley: Genome-Wide Identification, Evolution, Expression and Haplotype Analysis

Pan

Zeng

et al. 2021

Preprint

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Background: CCCH transcription factors are important zinc finger transcription factors involved in the response to biotic and abiotic stress and physiological and developmental processes. Barley (Hordeum vulgare) is an agriculturally important cereal crop with multiple uses, such as brewing production, animal feed, and human food. The identification and assessment of new functional genes are important for the molecular breeding of barley. Results: In this study, a total of 35 protein-encoding CCCH genes unevenly dispersed on seven different chromosomes were identified in barley. Phylogenetic analysis categorized the barley CCCH genes (HvC3Hs) into seven subfamilies according to their distinct features, and this classification was supported by intron–exon structure and conserved motif analysis. Despite the large genome size of barley, the lower number of CCCH genes in barley might be attributed to the low frequency of segmental and tandem duplication events. Furthermore, the HvC3H genes displayed distinct expression profiles for different developmental processes and in response to various types of stresses. The expression of HvC3H9 was significantly induced by multiple types of abiotic stress and/or phytohormone treatment, which might make it an excellent target for the molecular breeding of barley. Genetic variation of HvC3Hs was characterized using publicly available exome-capture sequencing datasets. Clear genetic divergence was observed between wild and landrace barley populations in HvC3H genes. For most HvC3Hs, nucleotide diversity and the number of haplotype polymorphisms decreased during barley domestication. Conclusion: Overall, our study provides a comprehensive characterization of barley CCCH transcription factors, their diversity, and their biological functions.

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DgC3H1, a CCCH zinc finger protein gene, confers cold tolerance in transgenic chrysanthemum

Cited by 16 publications

References 29 publications

CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

The Roles of CCCH Zinc-Finger Proteins in Plant Abiotic Stress Tolerance

CCCH Zinc Finger Genes in Barley: Genome-Wide Identification, Evolution, Expression and Haplotype Analysis

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