Dextran-stabilized Fe-Mn bimetallic oxidase-like nanozyme for total antioxidant capacity assay of fruit and vegetable food

Han, Ximei; Liu, Ling; Gong, Huiyu; Luo, Linpin; Han, Yaru; Fan, Jingyuan; Xu, Chenfei; Yue, Tianli; Wang, Jianlong; Zhang, Wentao

doi:10.1016/j.foodchem.2021.131115

Cited by 45 publications

(19 citation statements)

References 37 publications

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“…A good linear detection could be achieved and the detection results were visually reflected well. As shown in Table 1 , this method is more general than most TAL assays that only detect ascorbic acid [ 44 – 47 ].…”

Section: Resultsmentioning

confidence: 99%

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Construction of Pd Single Site Anchored on Nitrogen-Doped Porous Carbon and Its Application for Total Antioxidant Level Detection

Zhang

et al. 2022

Nanoscale Res Lett

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Natural enzymes have excellent catalytic activity. However, due to their unstable nature and high cost, current research has turned to the synthesis and development of enzyme-like nanomaterials and single-atomic nanozymes. In this study, a single-atomic palladium-loaded nitrogen-doped porous carbon catalyst (SA-Pd/NPC) was prepared and used as a mimetic peroxidase to catalyze the substrates oxidation. The catalytic capability of the SA-Pd/NPC was tested by the TMB-H2O2 system, and it expressed a superior catalytic capability owing to the plentiful catalytic centers of the single-atom Pd, its high porosity, the large specific surface area, and the strong electron transfer capability of the NPC. For the color reaction of TMB, thiol antioxidants (e.g., glutathione, GSH) and non-thiol antioxidants (e.g., ascorbic acid, AA) are suitable for different inhibition mechanisms. GSH and AA are typical substances of these two main antioxidant types, respectively. Here, we demonstrate that this prepared catalyst could be used to simultaneously determine a variety of major known physiologically relevant thiol-containing and thiol-free antioxidants, accompanied by a blue color gradient change with UV–Vis spectra at 652 nm through the SA-Pd/NPC-catalyzed TMB-H2O2 system. Linear responses to GSH and AA could be obtained in the concentration ranges of 0.01–0.10 mM and 1–13 μM (both R2 values were greater than 0.970), respectively, while the limits of detection were 3 μM and 0.3 μM, respectively. The ability of the nanozyme to detect overall antioxidant levels (TAL) was also confirmed in subsequent tests on artificial saliva and biological samples.

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Section: Resultsmentioning

confidence: 99%

“…As shown in Table 1 , this method is more general than most TAL assays that only detect ascorbic acid [ 44 – 47 ].…”

Section: Resultsmentioning

confidence: 99%

Construction of Pd Single Site Anchored on Nitrogen-Doped Porous Carbon and Its Application for Total Antioxidant Level Detection

Zhang

et al. 2022

Nanoscale Res Lett

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“…The nanozyme-based colorimetric sensor was based on the OXD-like activity of CoOOH NSs being inhibited by SO 3 2− , and it showed a remarkable analytical performance to detect SO 3 2− in sugar, biscuits, and beer with a LOD as low as 2 × 10 −8 M. 21 Zhang et al synthesized gold doped copper hexacyanoferrate (Au@Cu-HCF) as POD-like nanozymes to detect the total antioxidant capacity (TAC) of some agroproducts, and the nanozymes realized LODs for the TAC of 4.61 mM in lotus root, 6.35 mM in citrus juice, and 1.00 mM in lemon beverage. 146 Moreover, Han et al prepared a 2D Fe−Mn bimetallic OXD-like nanozyme to detect the TAC of fruit and vegetable agroproducts, achieving a LOD as low as 1.17 μM and linear range of 1−30 μM (Figure 3A), 73 while Cai et al developed MnO 2 NSs with OXD-like activity to construct a lateral flow immunoassay for the detection of AFB 1 in maize, achieving a LOD as low as 15 pg mL −1 (Figure 3B). 147 Huang et al reported GMP-Cu nanozymes with LAC-like activity that could be significantly enhanced by sulfide.…”

Section: Nanozymes In Agroproductsmentioning

confidence: 99%

“…Afterward, methanol molecules originated from the recombination of Cu-bound OH groups with CH 3 radicals . Moreover, ROS intermediates also originated from dissolved oxygen molecules, which were adsorbed and activated by nanozymes with large surface areas. − This type of nanozyme, including metal NPs, nanostructured metal oxides, MOFs, and nanohybrids, is also mainly applied in the development of agricultural nanosensors. For example, Pt NPs, MnO 2 NSs, Ce-BPyDC MOFs, and 2D Fe–Mn bimetallic nanozymes have been exploited for the development of agricultural nanosensors. − …”

Section: Classifications Of Nanozymes In Sustainable Agriculturementioning

confidence: 99%

Lighting Up Agricultural Sustainability in the New Era through Nanozymology: An Overview of Classifications and Their Agricultural Applications

Cui

Zhang

et al. 2022

J. Agric. Food Chem.

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With the concept of sustainable agriculture receiving increasing attention from humankind, nanozymes, nanomaterials with enzyme-like activity but higher environmental endurance and longer-term stability than natural enzymes, have enabled agricultural technologies to be reformative, economic, and portable. Benefiting from their multiple catalytic activities and renewable nanocharacteristics, nanozymes can shine in agricultural scenarios using enzyme engineering and nanoscience, acting as sustainable toolboxes to improve agricultural production and reduce the risk to agricultural systems. Herein, we comprehensively discuss the classifications of nanozymes applied in current agriculture, including peroxidase-like, oxidase-like, catalase-like, superoxide dismutase-like, and laccase-like nanozymes, as well as their biocatalytic mechanisms. Especially, different applications of nanozymes in agriculture are deeply reviewed, covering crop protection and nutrition, agroenvironmental remediation and monitoring, and agroproduct quality monitoring. Finally, the challenges faced by nanozymes in agricultural applications are proposed, and we expect that our review can further enhance agricultural sustainability through nanozymology.

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“…Optical assays owning distinct superiorities in simplicity, cost-effectiveness, and rapidity enable them to be broadly utilized in POC devices. − Recently, an emerging class of nanomaterials with imitative enzyme features (called nanozymes) have attracted conspicuous attention. Thanks to their extraordinary catalytical performance, high stability, and low cost, nanozymes have been regarded as the ideal candidate for fabricating colorimetric sensors. − Until now, several nanozyme-based colorimetric approaches have been explored to assay liver-related biomarkers. For example, some inorganic nanomaterials, such as Au nanoclusters and Ce-metal organic frameworks, can serve as peroxidase mimics to induce chromogenic reactions for the determination of ALP.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel-Involved Colorimetric Platforms Based on Layered Double Oxide Nanozymes for Point-of-Care Detection of Liver-Related Biomarkers

Liu

Mei

Yang

et al. 2022

ACS Appl. Mater. Interfaces

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Monitoring the liver status in a convenient and low-cost way is significant for obtaining a warning about drug-indued liver diseases promptly. Herein, we designed a novel colorimetric point-ofcare (POC) platform for the determination of three liver-related biomarkersaspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP). This platform integrated agarose hydrogels into a portable device, where hydrogels were loaded with nanozymes and different reaction substances for triggering specific reactions and generating colorimetric signals. Typically, Au-decorated CoAl-layered double oxide (Au/LDO) was for the first time developed as the nanozyme with peroxidase (POD) mimic activity, which can accelerate the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxTMB with the coexistence of hydrogen peroxide (H 2 O 2 ). The detection mechanism of AST and ALT is based on the fact that they can cause individual cascade reactions to generate H 2 O 2 , and H 2 O 2 further activates the Au/LDO nanozyme to catalyze the chromogenic reaction of TMB. As for ALP, it can catalytically hydrolyze L-ascorbic acid-2-phosphate to ascorbic acid. The latter then discolored the oxTMB that was produced with the assistance of Au/LDO. Teaming up with a smartphone, the color information of hydrogels can be converted to hue values, which allow quantitative analysis of ALT, AST, and ALP with detection limits of 15, 10, and 5 U/L, respectively. Moreover, the simple and cost-effective platform was successfully applied for the simultaneous determination of the three analytes in human plasma. Additionally, since the hydrogel is disposable and can be replaced by new ones loaded with different reaction regents, the platform is expected to serve the POC testing of various chem/bio targets.

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Dextran-stabilized Fe-Mn bimetallic oxidase-like nanozyme for total antioxidant capacity assay of fruit and vegetable food

Cited by 45 publications

References 37 publications

Construction of Pd Single Site Anchored on Nitrogen-Doped Porous Carbon and Its Application for Total Antioxidant Level Detection

Construction of Pd Single Site Anchored on Nitrogen-Doped Porous Carbon and Its Application for Total Antioxidant Level Detection

Lighting Up Agricultural Sustainability in the New Era through Nanozymology: An Overview of Classifications and Their Agricultural Applications

Hydrogel-Involved Colorimetric Platforms Based on Layered Double Oxide Nanozymes for Point-of-Care Detection of Liver-Related Biomarkers

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