Dextran-coated superparamagnetic nanoparticles modified with folate for targeted drug delivery of camptothecin

Abstract

Drug delivery vehicles based on magnetic nanoparticles present a promising strategy for cancer treatment, due to controlled targeted treatment, high loading efficiency, and biosafety as compared to traditional drug delivery approaches. In the present study, superparamagnetic iron oxide (Fe3O4) nanoparticles (SPIONs) were synthesised by a co-precipitation approach, stabilised with dextran (DEX), and successfully conjugated with folate (FA) for delivery of camptothecin (CPT) in prostate cancer cells. Size and other characteristics of the modified nanoparticles were measured using scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta-potential. The results demonstrated that the prepared FA-DEX-SPIONs were spherical in shape with an average diameter of 63.31 nm. They had anticancer activity with high CPT loading efficiency in AT3B-1cancer cells, enabling the therapeutic activity of the drug via its active delivery and release at 37 °C in phosphate and citrate buffer solutions. MTT results exhibited no cytotoxicity on rat androgen independent prostate cancer (AT3B-1) and normal human prostate (RWPE-1) cells. In conclusion, FA-DEX-SPION composite is a promising candidate that could be used for the targeted release of CPT anti-cancer drug.

Smart Nanoformulation Based on Polymeric Magnetic Nanoparticles and Vincristine Drug: A Novel Therapy for Apoptotic Gene Expression in Tumors

“…This finding clearly demonstrates that FA-DEX-VNC-SPION is a really powerful carrier that can be used for Tera-1 cancer cell-targeted drugs as a delivery mechanism. The results were considered satisfactory for targeted applications and are in agreement with those previously published [ 9 , 11 , 13 , 15 ].…”

“…Dextran has been extensively and efficiently used mostly for various biomedical purposes. The DEX encapsulation of SPIONs provides desirable stability with no toxicity reported [ 11 , 12 , 13 ]. Besides that, an efficient nanostructured framework is provided for potential treatment delivery to the target [ 14 ].…”

Design and Synthesis of Multi-Functional Superparamagnetic Core-Gold Shell Nanoparticles Coated with Chitosan and Folate for Targeted Antitumor Therapy

“…The rapid response to the external magnetic field, the stable formulation, and the desired stability of SPIONs in solution make these particles suitable nanocarriers [ 38 ]. The capacity of SPION@Au-CS-FA NPs for DOX release was studied under different pH conditions of 5.4 and 7.4 at 37 °C in PBS [ 39 ]. The amounts of (DOX) drug released from FA-CS-SPION@Au NPs were detected by calculating the supernatant’s fluorescent emission intensity at various pH values ( Figure 3 a).…”

Study the Effect of Laser Wavelength on Polymeric Metallic Nanocarrier Synthesis for Curcumin Delivery in Prostate Cancer Therapy: In Vitro Study

“…Radiation of laser is focused on a metal target within the particular solution fabrication nanoparticles dispersion [19][20][21]. The structures and properties of the production are easily dominated by modifying conditions of experimental like solutions, external environment, target materials, and laser parameters, the process can be performed at normal temperature and pressure [22][23][24][25]. The purpose of this study is to prepare and synthesized nanoencapsulation as drug delivery by PLAL method with a new formulation of FA-CU-CS-Fe@Au NPs to treat prostate cancer (PC3) and normal cell line (RWPE-1).…”