Smart Nanoformulation Based on Polymeric Magnetic Nanoparticles and Vincristine Drug: A Novel Therapy for Apoptotic Gene Expression in Tumors

Abstract

Background: Advanced nanobiotechnology provides safe and efficient drug delivery systems to deliver chemotherapy that targets cancer cells efficiently. Methods: A polymeric-magnetic nanocarrier was composed of a dextran (DEX) shell, a superparamagnetic iron oxide (SPION) core and was conjugated with folate (FA) to carry the anticancer drug vincristine (VNC) in Tera-1 testicular tumor cells. The molecular mechanisms by which apoptosis was induced were analyzed using flow cytometry and qPCR, which exhibited anticancer activity of nanoparticles (NPs). Results: This nanocarrier revealed a controlled release of VNC in citrate and phosphate buffer solutions that were maintained at pH 5.5 and pH 7.4, respectively. The Inhibitory concentration (IC50) values were greater than 5 mg/mL and displayed ten times higher cytotoxicity than the comparable free drug concentration. The Caspase-9 and P53 expressions were increased, whereas P21 and AKt1 decreased noticeably in the treated cells. The results point to the possible activation of apoptosis following treatment with NPs loaded with vincristine.

Biocompatible Nanomaterials as an Emerging Technology in Reproductive Health; a Focus on the Male

“…Studies of nanosystems specifically aimed at TC are rare – perhaps due to its relatively low incidence – however, one such study by Al-Musawi et al (2021) , covalently attached the anticancer agent vincristine to a SPION and encased this entity in dextran with surface folic acid to develop a targeted and directable NP. These NPs demonstrated a 10-fold increase in toxicity compared to the free drug when applied to Tera-1 testicular tumor cells in vitro ( Al-Musawi et al, 2021 ).…”

“…Studies of nanosystems specifically aimed at TC are rare – perhaps due to its relatively low incidence – however, one such study by Al-Musawi et al (2021) , covalently attached the anticancer agent vincristine to a SPION and encased this entity in dextran with surface folic acid to develop a targeted and directable NP. These NPs demonstrated a 10-fold increase in toxicity compared to the free drug when applied to Tera-1 testicular tumor cells in vitro ( Al-Musawi et al, 2021 ). Attention has also increasingly focused on the longer-term treatment sequelae as up to 60% of survivors of TC face the aforementioned consequences of lowered testosterone levels corresponding to the cumulative dose of cisplatin ( Poniatowska et al, 2020 ).…”

Study the Effect of Laser Wavelength on Polymeric Metallic Nanocarrier Synthesis for Curcumin Delivery in Prostate Cancer Therapy: In Vitro Study

“…Figure (2-b) showed the FA-CU-CS-Fe@Au NPs synthesized with an average size of 212.8 nm. To investigate the ability of the gold shell to keep the Fe core from, oxidation [28]. 4) presented for 96 h releases CU from (FA-CU-CS-Fe@Au NPs) and the result shows that the release time is faster at pH (5.4), when compared with pH (7.4).…”

Cytotoxicity Effect and Antibacterial Activity of Al2O3 Nanoparticles Activity against Streptococcus Pyogenes and Proteus Vulgaris

“…The significant interest of nanomaterial research and development, particularly NPs, is due to their attractive characteristics, revealed by their small size, unusual high surface area activity offering excellent catalytic, optical, and electrical properties [8][9][10]. Therefore, Metallic NPs have been active in comprehensive research, methods, and advanced micro-and nanotechnology applications [1,[11][12][13][14][15][16]. Due to their unique physicochemical properties in biological applications, metal oxide NPs like iron oxide NPs (Fe3O4), manganese oxide NPs (MgO), titanium dioxide NPs (TiO2), zinc oxide NPs (ZnO).…”