Dextran/Albumin hydrogel sealant for Dacron® vascular prosthesis

Abstract

In this paper, the authors describe a novel type of hydrogel coating prepared from the copolymer of human serum albumin and oxidized dextran. The material was designed as a hydrogel sealant for polyester (Dacron®)-based vascular grafts. Dextran was chosen as a coating material due to its anti-thrombogenic properties. Prepared hydrogels were compared with similar, already known biomaterial made from gelatine with the same cross-linking agent. Obtained hydrogels, prepared from various ratios of oxidized dextran/albumin or oxidized dextran/gelatine, showed different cross-linking densities, which caused differences in swelling, degradation rate and mechanical properties. Permeability tests confirmed the complete tightness of the hydrogel-modified prosthesis. Results showed that application of the hydrogel coating provided leakage-free prosthesis and eliminated the need of pre-clotting.

Detection of fluorescent organic nanoparticles by confocal laser endomicroscopy in a rat model of Barrett’s esophageal adenocarcinoma

“…In this system, the growth of dye In water, oxidized dextran can be reacted with molecules bearing a free amino group, to form biocompatible hydrogel coatings. 34 The method is highly versatile and allows the binding of a variety of biologically-active molecules. In the present case, peptides were grafted on the fluorescent polysaccharide NPs using this Schiff base reaction.…”

Albumin‐based hydrogels for regenerative engineering and cell transplantation

“…Chemical crosslinking is the most reported method to derive albumin hydrogels (Abbate, Kong, & Bansal, ; Bai et al, ; Feldman & McCauley, ; Gallego, Junquera, Meana, Álvarez‐Viejo, & Fresno, ; Gallego, Junquera, Meana, García, & García, ; He, Jean‐Francois, & Fortier, ; Hirose, Tachibana, & Tanabe, ; Kim et al, ; Li et al, ; Lisman, Butruk, Wasiak, & Ciach, ; Ma et al, ; Manokruang & Lee, ; Noteborn, Gao, Jesse, Kros, & Kieltyka, ; Oss‐Ronen & Seliktar, ; Overby & Feldman, ; Raja, Thiruselvi, Mandal, & Gnanamani, ; Scholz et al, ; Upadhyay & Rao, ; Zhao et al, ; Zhou et al, ). Synthetic polymers such as polyethylene glycol (PEG) are activated to form PEG‐albumin complexes (e.g., with 4‐nitrophenyl‐chloroformate), or alternatively functional groups may be added to the ends of the PEG molecule to target specific chemical compositions or binding sites of other target proteins for conjugation.…”

“…This can be circumvented by creating a porous scaffold through sacrificial molding then seeding cells within it (Shirahama et al, ). Other less‐commonly used agents to crosslink albumin include glutaraldehyde (Gallego, Junquera, Meana, Álvarez‐Viejo, et al, ; Gallego, Junquera, Meana, García, et al, ; Ma et al, ; Upadhyay & Rao, ; Zhao et al, ), glutathione (Bai et al, ; Raja et al, ), dithiothreitol (Hirose et al, ), transglutaminase (Li et al, ), polyaminourethane (Manokruang & Lee, ), oxidized dextran (Lisman et al, ), and N , N ‐methylenebisacrylamide (Abbate et al, ).…”

“…Apart from human MSCs, human skin fibroblasts (BJ‐5ta; Ribeiro et al, ) and mouse adipose fibroblasts (L929; Hirose et al, ; Lisman et al, ) have also been cultured successfully on albumin hydrogels but these were used only in the context of cytotoxicity testing.…”

Hydrogel‐coated polyurethane/urea shape memory polymer foams

“…Therefore, the pores in these grafts must be sealed initially. Typically, “preclotting” the pores with blood, which introduces its own set of thrombosis risks, is used . It has been proposed to seal the pores of the graft with hydrogels, as they are cheaper than protein‐based plugs .…”