Dexamethasone‐Conjugated Polyamidoamine Dendrimer for Delivery of the Heme Oxygenase‐1 Gene into the Ischemic Brain

Jeon, Pureum; Choi, Manbok; Oh, Jungju; Lee, Minhyung

doi:10.1002/mabi.201500058

Cited by 30 publications

(23 citation statements)

References 15 publications

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“…In previous studies, PAMAM-Dexa and PEI-Dexa were shown to have an anti-inflammatory effect due to the dexamethasone moiety (17). The anti-inflammatory effect of dexamethasone is beneficial to decrease the inflammatory reaction in the ischemic brain (10).…”

Section: Characterization Of Pamam G2-dexamentioning

confidence: 97%

“…Preparation of pDNA pβ-Luc, pSV-HO-1, and pEpo-SV-HO-1 were constructed previously (17,25). pEmpty was pSI, which was purchased from Promega (Madison, WI, USA).…”

Section: Synthesis Of Pamam-dexa and Pei-dexamentioning

confidence: 99%

“…Conjugation of dexamethasone to PAMAM G2 and PEI2k was performed as described previously (17). Briefly, PAMAM G2 or PEI2k was mixed with 2 equivalents of Traut's reagent and 2 equivalents of dexamethasone 21-mesylate (Seraloids, Saint Louis, MO, USA) in anhydrous DMSO.…”

Section: Synthesis Of Pamam-dexa and Pei-dexamentioning

confidence: 99%

“…To enhance the anti-inflammatory effect, dexamethasone was also conjugated to PAMAM G2 (16,17), since PAMAM is reported to have an anti-inflammatory effect by reducing proinflammatory cytokine levels (18). As a result, dexamethasone-conjugated PAMAM G2 (PAMAM-Dexa) had higher anti-inflammatory effect than PEI-Dexa (17). In addition, the gene delivery efficiency of PAMAM-Dexa was higher than that of PEI-Dexa.…”

Section: Introductionmentioning

confidence: 95%

“…Furthermore, the dexamethasone in PEI-Dexa has an anti-inflammatory effect (10,14,15), indicating that PEI-Dexa might be an anti-inflammatory drug as well as a gene carrier. To enhance the anti-inflammatory effect, dexamethasone was also conjugated to PAMAM G2 (16,17), since PAMAM is reported to have an anti-inflammatory effect by reducing proinflammatory cytokine levels (18). As a result, dexamethasone-conjugated PAMAM G2 (PAMAM-Dexa) had higher anti-inflammatory effect than PEI-Dexa (17).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Delivery of Hypoxia-Inducible Heme Oxygenase-1 Gene for Site-Specific Gene Therapy in the Ischemic Stroke Animal Model

Choi

Rhim

et al. 2016

Pharm Res

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Section: Characterization Of Pamam G2-dexamentioning

confidence: 97%

“…Preparation of pDNA pβ-Luc, pSV-HO-1, and pEpo-SV-HO-1 were constructed previously (17,25). pEmpty was pSI, which was purchased from Promega (Madison, WI, USA).…”

Section: Synthesis Of Pamam-dexa and Pei-dexamentioning

confidence: 99%

Section: Synthesis Of Pamam-dexa and Pei-dexamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Delivery of Hypoxia-Inducible Heme Oxygenase-1 Gene for Site-Specific Gene Therapy in the Ischemic Stroke Animal Model

Choi

Rhim

et al. 2016

Pharm Res

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Dendrimers as Powerful Building Blocks in Central Nervous System Disease: Headed for Successful Nanomedicine

Leiro

Santos

Lopes

et al. 2017

Adv Funct Materials

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Dendrimers have emerged as a powerful class of nanomaterials in the nanomedicine field due to their unique structural features: globular, welldefined, highly branched and controllable structure, nanosizescale, low polydispersity, and the presence of several terminal groups that can be functionalized with different ligands simulating the multivalency present in different biological systems. Although in its infancy, the application of dendrimers as therapeutics or theranostic tools in central nervous system (CNS) disorders is already significant and has opened promising avenues in the treatment of many conditions where the inherent "smartness" of the dendritic structures is being explored to effectively target the CNS. Here we present an overview of the past and future challenges of the use of dendrimers to respond to one of the ultimate challenges in the (nano)medicine field: to attain CNS repair and regeneration.

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Combined Delivery of a Lipopolysaccharide‐Binding Peptide and the Heme Oxygenase‐1 Gene Using Deoxycholic Acid‐Conjugated Polyethylenimine for the Treatment of Acute Lung Injury

Kim

Piao

Kim

et al. 2017

Macromolecular Bioscience

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A ternary complex comprising plasmid DNA, lipopolysaccharide-binding peptide (LBP), and deoxycholic acid-conjugated polyethylenimine (PEI-DA) is prepared for combinational therapy of acute lung injury (ALI). The LBP is designed as an anti-inflammatory peptide based on the lipopolysaccharide (LPS)-binding domain of HMGB-1. In vitro cytokine assays show that LBP reduces levels of proinflammatory cytokines by inhibiting LPS. PEI-DA is synthesized as the gene carrier by conjugation of deoxycholic acid to low-molecular weight polyethylenimine (2 kDa, PEI2k). PEI-DA has higher transfection efficiency than high-molecular weight polyethylenimine (25 kDa, PEI25k). The ternary complex of an HO-1 plasmid (pHO-1), PEI-DA, and LBP is prepared as a combinational system to deliver the therapeutic gene and peptide. The transfection efficiency of the ternary complex is higher than that of the pHO-1/PEI-DA binary complex. The ternary complex also reduces TNF-α secretion in LPS-activated Raw264.7 macrophage cells. Administration of the ternary complex into the lungs of an animal ALI model by intratracheal injection induces HO-1 expression and reduces levels of proinflammatory cytokines more efficiently than the pHO-1/PEI-DA binary complex or LBP alone. In addition, the ternary complex reduces inflammation in the lungs. Therefore, the pHO-1/PEI-DA/LBP ternary complex may be an effective treatment for ALI.

show abstract

Dexamethasone‐Conjugated Polyamidoamine Dendrimer for Delivery of the Heme Oxygenase‐1 Gene into the Ischemic Brain

Cited by 30 publications

References 15 publications

Delivery of Hypoxia-Inducible Heme Oxygenase-1 Gene for Site-Specific Gene Therapy in the Ischemic Stroke Animal Model

Delivery of Hypoxia-Inducible Heme Oxygenase-1 Gene for Site-Specific Gene Therapy in the Ischemic Stroke Animal Model

Dendrimers as Powerful Building Blocks in Central Nervous System Disease: Headed for Successful Nanomedicine

Combined Delivery of a Lipopolysaccharide‐Binding Peptide and the Heme Oxygenase‐1 Gene Using Deoxycholic Acid‐Conjugated Polyethylenimine for the Treatment of Acute Lung Injury

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