Combined Delivery of a Lipopolysaccharide‐Binding Peptide and the Heme Oxygenase‐1 Gene Using Deoxycholic Acid‐Conjugated Polyethylenimine for the Treatment of Acute Lung Injury

Kim, Ji Yeon; Piao, Chunxian; Kim, Gyeungyun; Lee, Seon Yeong; Lee, Min Sang; Jeong, Ji Hoon; Lee, Minhyung

doi:10.1002/mabi.201600490

Cited by 17 publications

(8 citation statements)

References 34 publications

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“…Recombinant gp12 (the T4 phage tail adhesin) retains the ability to bind LPS, and gp12 was successfully used in an in vivo experiment to counteract LPS-induced inflammation in rice ( Miernikiewicz et al, 2016 ). Kim et al (2017) demonstrated that a non-toxic LPS binding peptide was an effective anti-inflammatory peptide for the treatment of acute lung injury. Brackett et al (1997) demonstrated that a synthetic peptide corresponding to amino acid residues 20 through 44 of the neutrophil-derived 37-kDa cationic antimicrobial protein (CAP37 P20–44) can bind lipid A of LPS, which could be useful in attenuating in vivo responses induced during endotoxemia, including sepsis.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Tail Sheath Protein of N4-Like Phage phiAxp-3

et al. 2018

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Achromobacter phage phiAxp-3, an N4-like bacteriophage, specifically recognize Achromobacter xylosoxidans lipopolysaccharide (LPS) as its receptor. PhiAxp-3 tail sheath protein (TSP, ORF69) shares 54% amino acid sequence identity with the TSP of phage N4 (gp65); the latter functions as a receptor binding protein and interacts with the outer membrane receptor NfrA of its host bacterium. Thus, we hypothesized that ORF69 is the receptor-binding protein of phiAxp-3. In the present study, a series of ORF69 truncation variants was constructed to identify the part(s) of this protein essential for binding to A. xylosoxidans LPS. Phage adsorption and enzyme-linked immunosorbent assay showed that amino acids 795–1195 of the TSP, i.e., ORF69(795–1195), are sufficient and essential for receptor and binding. The optimum temperature and pH for the functions of ORF69 and ORF69(795–1195) are 4/25°C and 7, respectively. In vitro cytotoxicity assays showed that ORF69 and ORF69(795–1195) were respectively toxic and non-toxic to a human immortalized normal hepatocyte cell line (LO2; doses: 0.375–12 μg). The potential of this non-toxic truncated version of phiASP-3 TSP for clinical applications is discussed.

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Section: Discussionmentioning

confidence: 99%

Characterization of Tail Sheath Protein of N4-Like Phage phiAxp-3

et al. 2018

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“…claimed that increased bacteria-derived 5-hydroxytryptophan (5-HT) in the peripheral circulation activates the formation of pulmonary NETs as well ( 129 ). While, several metabolites from intestinal bacteria, such as bile acids (ocaliva and ursodeoxycholic acid), butyric acid, etc., are proven to have protective effects on PALI ( 130 – 132 ).…”

Section: Mechanisms Of Ali Caused By Intestinal Bacterial Dysbiosismentioning

confidence: 99%

Intestinal Microbiota - An Unmissable Bridge to Severe Acute Pancreatitis-Associated Acute Lung Injury

Wang

Liu

et al. 2022

Front. Immunol.

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Severe acute pancreatitis (SAP), one of the most serious abdominal emergencies in general surgery, is characterized by acute and rapid onset as well as high mortality, which often leads to multiple organ failure (MOF). Acute lung injury (ALI), the earliest accompanied organ dysfunction, is the most common cause of death in patients following the SAP onset. The exact pathogenesis of ALI during SAP, however, remains unclear. In recent years, advances in the microbiota-gut-lung axis have led to a better understanding of SAP-associated lung injury (PALI). In addition, the bidirectional communications between intestinal microbes and the lung are becoming more apparent. This paper aims to review the mechanisms of an imbalanced intestinal microbiota contributing to the development of PALI, which is mediated by the disruption of physical, chemical, and immune barriers in the intestine, promotes bacterial translocation, and results in the activation of abnormal immune responses in severe pancreatitis. The pathogen-associated molecular patterns (PAMPs) mediated immunol mechanisms in the occurrence of PALI via binding with pattern recognition receptors (PRRs) through the microbiota-gut-lung axis are focused in this study. Moreover, the potential therapeutic strategies for alleviating PALI by regulating the composition or the function of the intestinal microbiota are discussed in this review. The aim of this study is to provide new ideas and therapeutic tools for PALI patients.

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“…Kim et al reported the synthesis of DCA conjugated polyethyleneimine (PEI) through post-polymerization modulation on PEI. [78] Dicyclohexylcarbodiimide (DCC) and NHS were employed during the coupling process. The resultant PEI-DCA material leads to the formation of a ternary complex along with lipopolysaccharide-binding peptide (LBP) and plasmid DNA, for synergistic action in combinatorial therapy.…”

Section: Design Trends Of Deoxycholic Acid-based Polymersmentioning

confidence: 99%

Recent Progress in Macromolecular Design and Synthesis of Bile Acid‐Based Polymeric Architectures

Sahoo

Ghosh

Khan

et al. 2021

Macro Chemistry & Physics

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Macromolecular engineering presents a pleasant tactic for planning the construction of desired molecular architectures from nano to macro configurations. The mechanistic pathways and synthetic features of macromolecular designs mostly rely on the precursor composition, topology, functionality, and complexity in architectures. Specifically, macromolecular design with biomolecular conjugation has attracted remarkable attention due to its typical biochemical and physiological affirmative traits. In this review article, it is intended to highlight the major progress on the macromolecular designs and synthesis of polymers comprising bile acid (BA) as the biomolecular colleague. BAs are steroidal biocomposites, produced through cholesterol metabolism in the bile of mammals and vertebrates. They are low priced, biocompatible, abundant, trivially toxic, and structurally unique for facewise amphiphilicity, which make them suitable for designing BA-based polymers (BAPs) to be utilized in biomedical fields. The major aim of this review article is to summarize the recent progress (2015-present) on the requisite necessity of BA in BAPs toward design and synthetic avenues. In particular, the design of various polymers with primary and secondary BAs at side chain, main chain, chain end, crosslinking zone, and core confined area are illustrated in this review article via numerous reported methods.

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Combined Delivery of a Lipopolysaccharide‐Binding Peptide and the Heme Oxygenase‐1 Gene Using Deoxycholic Acid‐Conjugated Polyethylenimine for the Treatment of Acute Lung Injury

Cited by 17 publications

References 34 publications

Characterization of Tail Sheath Protein of N4-Like Phage phiAxp-3

Characterization of Tail Sheath Protein of N4-Like Phage phiAxp-3

Intestinal Microbiota - An Unmissable Bridge to Severe Acute Pancreatitis-Associated Acute Lung Injury

Recent Progress in Macromolecular Design and Synthesis of Bile Acid‐Based Polymeric Architectures

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