Dewetting of Polystyrene Thin Films on Poly(ethylene glycol)-Modified Surfaces as a Simple Approach for Patterning Proteins

Cai, Yuzhe; Newby, Bi‐min Zhang

doi:10.1021/la703923z

Cited by 27 publications

(18 citation statements)

References 42 publications

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“…20,21,27,28 The role of surface topography in protein adsorption and cell behavior has also been widely studied 19,29,30 ; in general, it is difficult to differentiate between the relative effect of topography and chemistry on protein adsorption and cell behavior. 20 In the present study, measurements for surface roughness revealed that differences for surface roughness values between the studied substrates were significant (P b .05) in all cases, except when comparing glass and PDMS.…”

Section: Discussionmentioning

confidence: 99%

The role of surface energy of technical polymers in serum protein adsorption and MG-63 cells adhesion

Comelles

Estévez

Martínez

et al. 2010

Nanomedicine: Nanotechnology, Biology and Medicine

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Section: Discussionmentioning

confidence: 99%

The role of surface energy of technical polymers in serum protein adsorption and MG-63 cells adhesion

Comelles

Estévez

Martínez

et al. 2010

Nanomedicine: Nanotechnology, Biology and Medicine

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“…30 Briefly, cleaned glass slides (1 Â 1 cm 2 ) were oxidized with UV/ozone oxidation for 6 min. 30 Briefly, cleaned glass slides (1 Â 1 cm 2 ) were oxidized with UV/ozone oxidation for 6 min.…”

Section: B Surface Preparationmentioning

confidence: 99%

Applicability of the extended Derjaguin–Landau–Verwey–Overbeek theory on the adsorption of bovine serum albumin on solid surfaces

Hua

Newby

2014

Biointerphases

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Protein adsorption is the prerequisite for bacterial attachment and cellular adhesion, which are critical for many biomedical applications. To understand protein adsorption onto substrates, predictive models are generally informative prior to experimental studies. In this study, the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory was employed to determine whether or not it could interpret the protein adsorption behaviors. The experimental results of fluorescein isothiocyanate labeled bovine serum albumin (BSA) adsorbed on six different surfaces: glass, octadecyltrichlorosilane modified glass, 2-[methoxypoly(ethyleneoxy)propyl]trimethoxy-silane (PEG)-modified glass, polystyrene, poly(dimethylsiloxane), and poly(methyl methacrylate) were utilized. The XDLVO interaction energy curves, especially from the contribution of acid-base interactions, obtained using the surface properties of substrates and BSA molecules qualitatively predict/interpret the protein adsorption behaviors on these surfaces. Some derivation of the experimental results from the prediction was noticed for the glass and the PEG-modified glass. When including a hydration layer to the PEG-modified glass surface, the nonfouling result of such surface by proteins was also elucidated by the XDLVO theory.

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“…Other groups 21,22 have used polymer blend systems to study the selective protein adsorption on phase-separated polymer surfaces. Meanwhile, researchers 23,24 have patterned proteins on templates created by the controlled dewetting of the top film in a bilayer consisting of a hydrophobic polymer and a hydrophilic polymer.…”

Section: Introductionmentioning

confidence: 99%

Protein Nanopatterning on Self-Organized Poly(styrene-b-isoprene) Thin Film Templates

2009

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Abstract:Templated surfaces can be used to create patterns of proteins for applications in cell biology, bio-sensors and tissue engineering. A diblock copolymer template, which contains a pair of hydrophobic blocks, has been developed. The template is created from well-ordered, non-equilibrium surface structures of poly(styrene-b-isoprene) (PS-b-PI) diblock copolymers, which are achieved in ultrathin films having a thickness less than one domain period. Adsorption and nanopatterning of bovine serum albumin (BSA) on these thin films were studied. After incubation of the copolymer templates in BSA solutions (500 µg/ml) for a period of one hour, BSA molecules formed either a striped or a dense, ring-like structure, closely resembling the underlying polymer templates. In this "hard-soft" PS-b-PI system, BSA molecules were preferentially adsorbed on the hard PS domains, rather than on the soft PI domains. SIMS and contact angle analysis revealed that with more PI localized at the free surface, fewer BSA molecules were adsorbed. SIMS analysis confirmed that BSA molecules were adsorbed selectively on the PS blocks. This is the first example of two hydrophobic blocks of a diblock copolymer being used as a protein patterning template. Previously reported diblock copolymer templates used hydrophilic and hydrophobic pairs. A potentially useful characteristic of this template is that it is effective at high protein solution concentrations (up to 1 mg/ml) and for long incubation times (up to two hours), which broadens its range of applicability in various uses.

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Dewetting of Polystyrene Thin Films on Poly(ethylene glycol)-Modified Surfaces as a Simple Approach for Patterning Proteins

Cited by 27 publications

References 42 publications

The role of surface energy of technical polymers in serum protein adsorption and MG-63 cells adhesion

The role of surface energy of technical polymers in serum protein adsorption and MG-63 cells adhesion

Applicability of the extended Derjaguin–Landau–Verwey–Overbeek theory on the adsorption of bovine serum albumin on solid surfaces

Protein Nanopatterning on Self-Organized Poly(styrene-b-isoprene) Thin Film Templates

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