Applicability of the extended Derjaguin–Landau–Verwey–Overbeek theory on the adsorption of bovine serum albumin on solid surfaces

Abstract

Protein adsorption is the prerequisite for bacterial attachment and cellular adhesion, which are critical for many biomedical applications. To understand protein adsorption onto substrates, predictive models are generally informative prior to experimental studies. In this study, the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory was employed to determine whether or not it could interpret the protein adsorption behaviors. The experimental results of fluorescein isothiocyanate labeled bovine serum albumin (BSA) adsorbed on six different surfaces: glass, octadecyltrichlorosilane modified glass, 2-[methoxypoly(ethyleneoxy)propyl]trimethoxy-silane (PEG)-modified glass, polystyrene, poly(dimethylsiloxane), and poly(methyl methacrylate) were utilized. The XDLVO interaction energy curves, especially from the contribution of acid-base interactions, obtained using the surface properties of substrates and BSA molecules qualitatively predict/interpret the protein adsorption behaviors on these surfaces. Some derivation of the experimental results from the prediction was noticed for the glass and the PEG-modified glass. When including a hydration layer to the PEG-modified glass surface, the nonfouling result of such surface by proteins was also elucidated by the XDLVO theory.

The Bacterial Life Cycle in Textiles is Governed by Fiber Hydrophobicity

“…4 ). This corresponds well with the XDLVO theory, which predicts that bacteria adhere more strongly to more hydrophobic surfaces ( 2 ). The XDLVO theory is a simplification of bacterial attachment, as it considers bacteria as colloidal particles and predicts their interactions based on average physicochemical properties while ignoring specific interactions of surface appendages that differ from the average cell surface properties.…”

“…This force is sufficient to drive the bacterium to adhere irreversibly once the water film is thin enough relative to the cell diameter. The capillary force can create a potential that is 2 orders of magnitude larger than the energy barrier to irreversible adhesion created by the XDLVO potential ( 2 ). This is likely to make the size of the XDLVO energy barrier (and therefore the hydrophobicity of the surface) irrelevant to adhesion during drying.…”

“…We hypothesize that biofilms will establish faster on the more adhesive fibers, which will affect the bacterial behavior during the other stages of the life cycle. The physics of bacterial adhesion to surfaces can be described by the extended Derjaguin, Landau, Verwey, and Overbeek (XDLVO) theory ( 2 ), which predicts that bacteria will adhere more strongly to more hydrophobic fibers. After adhesion, biofilm growth is limited by the availability of nutrients.…”

Understanding the interaction of proteins to ion exchange chromatographic supports: A surface energetics approach

“…A recent study is published that explains a comparison of different methods used for the determination of surface energies of colloidal particles 20 . This theory has also been used to study bovine serum interaction to different solid surfaces 33 . This theory has also been modified to study protein–protein interaction 34,35 …”

“…20 This theory has also been used to study bovine serum interaction to different solid surfaces. 33 This theory has also been modified to study protein-protein interaction. 34,35 All these studies suggest that the surface energetics approach, utilizing XDLVO theory, has the potential to understand protein interaction to ion exchange chromatographic supports, which has never been attempted earlier.…”