Devices based on light emitting fabrics dedicated to PDT preclinical studies

Abstract

Whether preclinical studies either involve a cell or animalmodel, the distribution of light plays a determinant role in the reproducibility of results of photodynamic therapy (PDT) studies. Unfortunately, only few illumination devices dedicated to preclinical studies are available and are for the most, very expensive. Mostresearch teams use home-made solutions that may not always be reproducible because of undefined light distribution, additive thermal emission, or unsuitable for shapes and volumes to illuminate.To address these issues, we developed illumination devices dedicated to our preclinical studies, which embed knitted light emitting fabrics (LEF) technology. LEF technology offers a homogeneous light distribution, without thermal emission and can be coupled with various light sources allowinginvestigation of several PDT modalities (irradiance, wavelength, illumination duration/mode).For in-vitro studies, we designed light plates,each allowing illumination of up to four 96-cells plates. For in-vivo studies, we designed mice boxes allowing three animals placement in prone position, equally surrounded by LEF and ensuring homogeneous extracorporeal illumination.Optical validation was performed and reproducibility of both preclinical systems were assessed.Both systems can deliver homogeneous light with an irradiance that can reachseveral mW/cm 2 , with varying durations and wavelengths. First results of preclinical studies demonstrate a high reproducibility, with an easy setup, and a great adaptability of illumination modalities with these devices based on light emitting fabrics.

An Efficient Photodynamic Therapy Treatment for Human Pancreatic Adenocarcinoma

“…PS2 was synthesized and provided by the team of Dr. Celine Frochot (Supplementary Figure S1). In vitro and in vivo illumination was performed as previously described with a specific device developed by OncoThAI research team [16]. For in vitro study, a homogenous illumination during 1 h at 1 mW/cm 2 (3.6 J/cm 2 ) was performed and for in vivo treatment, an extracorporeal fractionated illumination of 2 h 30 in total (1 min of illumination followed by 2 min of rest, repeated 45 times) at 11 mW/cm 2 (29.7 J/cm 2 ) was performed.…”

Photodynamic Therapy Using a New Folate Receptor-Targeted Photosensitizer on Peritoneal Ovarian Cancer Cells Induces the Release of Extracellular Vesicles with Immunoactivating Properties

“…A homogeneous illumination (1 mW/cm 2 ) with a 668 nm laser was then performed for 1 h, with a specific device developed by OncoThAI. Thanks to the device, 8 × 75 cm 2 or 25 cm 2 or 96-well plates could be illuminated simultaneously and homogeneously, as previously described [22]. After another 24 h, the supernatant was recovered, centrifuged, and then frozen at −20 • C, until further use.…”

Photodynamic therapy with light emitting fabrics: a review

“…This was connected to CELL-LEF which gave irradiance values ranging from 0.81 to 1.18 mW cm −2 with a homogeneity of 90.9%, much below the accessible emission limits of class 1 as per IEC 60,825 standard. The study also surpassed concerns regarding elevation in temperature during treatment as an illumination of 45 min led to an increase of only + 1.14 °C while the inside of the well recorded an increase of + 0.88 °C [17,18].…”