Developments in Serological Methods to Detect and Identify Plant Viruses

Torrance, L.

doi:10.1007/978-94-015-8951-2_8

Cited by 5 publications

(5 citation statements)

References 29 publications

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“…conversion of the chromogenic substrate p-nitrophenyl phosphate (colourless) to pnitrophenol (yellow in alkaline solution) that reveals the presence of bound enzyme. This assay can be very sensitive, detecting viruses at concentrations of 1-10 ng ml 71 (Torrance 1998). Assays in which antibodies or virus particles are bound to nitrocellulose membrane followed by the incubation with antibody-enzyme conjugates are known as immunoblots or dot blots.…”

Section: Serological Methodsmentioning

confidence: 99%

Technical advancement in plant virus diagnosis – an appraisal

Jan

Azam

Warsi

et al. 2012

Archives Of Phytopathology And Plant Protection

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Plants infected by a wide range of viruses often cause important agronomic and economic losses worldwide. Detection of plant viruses is becoming more challenging as globalisation of trade, particularly the ornamental plants, and the potential effects of climate change facilitate movement of viruses and their vectors, thereby transforming the diagnostic landscape. Under such circumstances, detection of viruses at premature stages of infection by use of rapid, sensitive and accurate detection methods seems crucial to ensure safe and sustainable agriculture and as such reduces economic losses. For proper identification, detection technique needs to be rapid, most accurate and inexpensive, as it forms the key step in developing appropriate practical solutions to manage plant virus diseases that pose continuous threats to the viability of certain horticultural and agricultural industries. This review is concerned with the advancement in techniques in the diagnosis of viruses in plants.

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Section: Serological Methodsmentioning

confidence: 99%

Technical advancement in plant virus diagnosis – an appraisal

Jan

Azam

Warsi

et al. 2012

Archives Of Phytopathology And Plant Protection

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“…Serological detection systems use specific antibody developed in animals in respond to antigens (Torrance, 1998). Viruses can be detected if viral antigens are used to develope antibody.…”

Section: Serological Methodsmentioning

confidence: 99%

A Review of Detection Methods for the Plant Viruses

Jeong¹,

Ju²,

Noh³

2014

Research in Plant Disease

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The early and accurate detection of plant viruses is an essential component to control those. Because the globalization of trade by free trade agreement (FTA) and the rapid climate change promote the country-tocountry transfer of viruses and their hosts and vectors, diagnosis of viral diseases is getting more important. Because symptoms of viral diseases are not distinct with great variety and are confused with those of abiotic stresses, symptomatic diagnosis may not be appropriate. From the last three decades, enzyme-linked immunosorbent assays (ELISAs), developed based on serological principle, have been widely used. However, ELISAs to detect plant viruses decrease due to some limitations such as availability of antibody for target virus, cost to produce antibody, requirement of large volume of sample, and time to complete ELISAs. Many advanced techniques allow overcoming demerits of ELISAs. Since the polymerase chain reaction (PCR) developed as a technique to amplify target DNA, PCR evolved to many variants with greater sensitivity than ELISAs. Many systems of plant virus detection are reviewed here, which includes immunological-based detection system, PCR techniques, and hybridization-based methods such as microarray. Some of techniques have been used in practical, while some are still under developing to get the level of confidence for actual use.

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“…The use of antibody technology is well established in medical diagnostics and interest in its use in plant pathology has been increasing over the last decade. Methods for producing antibodies for plant pathogen diagnostics and their use have been reviewed by several authors (Werres & Steffens 1994;Dewey & Thornton, 1995;Barker, 1996;Dewey et al, 1997;Torrance, 1998;Schots, 1995).…”

Section: Antibody-based Techniquesmentioning

confidence: 99%

Plant pathogen diagnostics: immunological and nucleic acid‐based approaches

Ward

Foster

Fraaije

et al. 2004

Annals of Applied Biology

186

121

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Accurate disease diagnosis and precise identification of any pathogens involved is an essential prerequisite for understanding plant diseases and controlling them effectively. Traditional methods of identifying plant pathogens can be slow and inconclusive, and this has prompted the search for alternative diagnostic techniques. Here, we review recent developments in antibody-based and nucleic acid-based diagnostic methods, and their applications in plant pathology. We also highlight some new developments in diagnostics that are likely to make an impact in the future.

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Developments in Serological Methods to Detect and Identify Plant Viruses

Cited by 5 publications

References 29 publications

Technical advancement in plant virus diagnosis – an appraisal

Technical advancement in plant virus diagnosis – an appraisal

A Review of Detection Methods for the Plant Viruses

Plant pathogen diagnostics: immunological and nucleic acid‐based approaches

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