Developments in Plant-Based Vaccines Against Diseases of Concern in Developing Countries

Gómez, Emma Laura Alfaro; Zoth, Silvina Chimeno; Carrillo, Elisa; Berinstein, Analía

doi:10.2174/1874279301004010055

Cited by 7 publications

(13 citation statements)

References 84 publications

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“…This method requires very high cost and can harm the plant. [14,41] Nuclear transformation and chloroplast transformation can be done by biolistic method. These were the two types of antigen expression method [42].…”

Section: Direct Gene Delivery Methodsmentioning

confidence: 99%

Edible Vaccines: Promises and Challenges

Kurup

Thomas

2019

Mol Biotechnol

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Vaccines are biological preparations that improve immunity to particular diseases and form an important innovation of 19th century research. It contains a protein that resembles a disease-causing microorganism and is often made from weak or killed forms of the microbe. Vaccines are agents that stimulate the body's immune system to recognize the antigen. Now, a new form of vaccine was introduced which will have the power to mask the risk side of conventional vaccines. This type of vaccine was produced from plants which are genetically modified. In the production of edible vaccines, the gene-encoding bacterial or viral disease-causing agent can be incorporated in plants without losing its immunogenic property. The main mechanism of action of edible vaccines is to activate the systemic and mucosal immunity responses against a foreign diseasecausing organism. Edible vaccines can be produced by incorporating transgene in to the selected plant cell. At present edible vaccine are developed for veterinary and human use. But the main challenge faced by edible vaccine is its acceptance by the population so that it is necessary to make aware the society about its use and benefits. When compared to other traditional vaccines, edible vaccines are cost effective, efficient and safe. It promises a better prevention option from diseases.

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Section: Direct Gene Delivery Methodsmentioning

confidence: 99%

Edible Vaccines: Promises and Challenges

Kurup

Thomas

2019

Mol Biotechnol

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“…For example, preclinical trials with influenza VLPs indicated their capacity to induce both humoral and cellular immune responses at low antigen doses. Several authors have reported antibody response to parenterally or orally administered plant‐derived antigens . As exogenous antigens, VLPs are taken up by professional antigen presenting cells (APCs), especially DCs, followed by antigen processing and presentation via MHC class II molecules, DC activation and maturation through up‐regulation of co‐stimulatory molecules and cytokine production, and stimulation of CD4+ T helper cells.…”

Section: Immunostimulation By Vlpsmentioning

confidence: 99%

Different applications of virus‐like particles in biology and medicine: Vaccination and delivery systems

2015

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Virus-like particles (VLPs) mimic the whole construct of virus particles devoid of viral genome as used in subunit vaccine design. VLPs can elicit efficient protective immunity as direct immunogens compared to soluble antigens co-administered with adjuvants in several booster injections. Up to now, several prokaryotic and eukaryotic systems such as insect, yeast, plant, and E. coli were used to express recombinant proteins, especially for VLP production. Recent studies are also generating VLPs in plants using different transient expression vectors for edible vaccines. VLPs and viral particles have been applied for different functions such as gene therapy, vaccination, nanotechnology, and diagnostics. Herein, we describe VLP production in different systems as well as its applications in biology and medicine.

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“…Traditional vaccines are produced by applying fermentation technology in various cell culture systems. However, numerous limitation related to fermentation so that development of alternative systems for the production of vaccines is timely (Davoodi et al, 2009;Daniell et al, 2009;Gomez et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Regeneration and Transformation of Gene Encoding the Hemagglutinin Antigen of the H5N1 Virus in Frond of Duckweed (Spirodela polyrhiza L.)

Thu

Huong

Tien

et al. 2015

JAS

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H5N1 (Avian influenza virus) has caused economic loss and remained a serious human health risk in many countries, including Vietnam. Plant-derived vaccines have offered the advantages over tradition subunit vaccine with low-cost production potential. Duckweed (Sprirodela polyrhiza L.) has found a valuable target plant for various application. In this study, we have attempted to re-generate and transform the gene encoding the hemagglutinin antigen of H5N1 virus in frond of S. polyrhiza, a widely great duckweed grown in Vietnam. The results have shown that the culture medium for S. polyrhiza duckweed is Hutner medium with reduced mineral concentration ½ (H/2) supplemented with 10g/l sucrose at pH 6. This medium showed appropriate for the best growth and development of duckweed. Results of PCR analysis of S. polyrhiza lines from 14 transformation experiments that has obtained 4 duckweed lines carrying HA1 gene. This prelinary results will be provide the basis for transgenic research in the dukweed species and be useful for further generate the producing enable vaccine via the transgenic plants

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Developments in Plant-Based Vaccines Against Diseases of Concern in Developing Countries

Cited by 7 publications

References 84 publications

Edible Vaccines: Promises and Challenges

Edible Vaccines: Promises and Challenges

Different applications of virus‐like particles in biology and medicine: Vaccination and delivery systems

Regeneration and Transformation of Gene Encoding the Hemagglutinin Antigen of the H5N1 Virus in Frond of Duckweed (Spirodela polyrhiza L.)

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