Developments in mycotoxin analysis: an update for 2010-2011

Shephard, Gordon S.; Berthiller, Franz; Burdaspal, P. A.; Crews, Colin; Jonker, Marco A.; Krska, Rudolf; MacDonald, Susan; Malone, R.J.; Maragos, Chris M.; Sabino, Myrna; Solfrizzo, Michele; Egmond, Hans van; Whitaker, T. B.

doi:10.3920/wmj2011.1338

Cited by 80 publications

(33 citation statements)

References 203 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Numerous studies in the recent years highlighted enzyme linked immunosorbent assay (ELISA) as the most frequently used technique for that purpose, followed by high performance liquid chromatography with fluorescence detector (HPLC-FLD) and liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). 18,19 Thin layer chromatography is almost abandoned as a technique since it is reliable for detection of AFM1 only for concentration above or around 0.05 μg/kg. 20 Further, recent studies have resulted in publication of a several biosensor-based methods for AFM1 determination in milk samples.…”

Section: Kos Et Al: Comparison Of Elisa Hplc-fld and Hplc-ms/ms mentioning

confidence: 99%

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

Kos

Hajnal²,

Jajić³

et al. 2016

ACSi

View full text Add to dashboard Cite

Presence of aflatoxin M1 (AFM1) in milk should be continuously controlled in order to protect the population from risks associated with its proven toxicity and carcinogenicity. During recent years, there has been an increase in demand for development of sensitive, accurate, simple and fast method which is reliable for detection of AFM1 at low concentrations found in milk samples. For that purpose, enzyme linked immunosorbent asssay (ELISA), high performance liquid chromatography with fluorescence detector (HPLC-FLD) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were optimized and validated in order to apply them for AFM1 analysis in naturally contaminated milk samples, and to assess the closeness of agreement between results of three different methods. The obtained validation parameters indicate that all three methods are suitable for determination of AFM1 in milk samples. The statistical analysis of variance between the methods and the obtained correlation coefficients indicate that there is a strong correlation between methods. All three methods are satisfactory in meeting the requirements for official control purposes. To the best of author's knowledge, this study represents the first report of an investigation and comparison of ELISA, HPLC-FLD and HPLC-MS/MS methods for determination of AFM1 in naturally contaminated milk samples.

show abstract

Section: Kos Et Al: Comparison Of Elisa Hplc-fld and Hplc-ms/ms mentioning

confidence: 99%

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

Kos

Hajnal²,

Jajić³

et al. 2016

ACSi

View full text Add to dashboard Cite

show abstract

“…About 5 billion people are estimated to suffer from uncontrolled exposure to aflatoxin worldwide (Strosnider et al 2006). The group of aflatoxins comprise B1, B2, G1 and G2 (Aflatoxin B: AFB1 and AFB2, Aflatoxin G: AFG1 and AFG2) (Shephard et al 2012), among which aflatoxin B1 is the most potent hepatotoxic and carcinogenic compound (Khlangwiset and Wu 2010;Yang et al 2012). Aspergilluis parasiticus is one of Aspergillus species that is able to produce aflatoxins under certain conditions (Khlangwiset and Wu 2010).…”

Section: Introductionmentioning

confidence: 99%

Antitoxin Characteristic of Licorice Extract: The Inhibitory Effect on Aflatoxin Production in Aspergillus parasiticus

Mohseni

Noorbakhsh

Moazeni

et al. 2014

Journal of Food Safety

View full text Add to dashboard Cite

Recent investigations revealed the effects of herbal extracts on both fungal growth as well as aflatoxins production. In the present study, we tried to evaluate antifungal activity as well as antitoxin activity of Glycyrrhiza glabra (licorice) extract. Strain American Type Culture Collection 15517 of Aspergillus parasiticus was used to perform antifungal susceptibility test according to Clinical and Laboratory Standards Institute document M27-A3, and the rate of aflatoxin production was determined using high-performance liquid chromatography technique after exposure to different concentrations of licorice extract. Quantitative changes in the expression of the aflR gene were analyzed by measuring the cognate aflR mRNA level with quantitative real-time reverse-transcription polymerase chain reaction assay. Our obtained results demonstrated the inhibitory effect of licorice extract on Aspergillus parasiticus growth at 500 mg/mL of licorice extract. In addition, a significant decrease in aflatoxin production was revealed at the same concentration. However, the production of aflatoxin B1 was entirely inhibited in 10 g/mL of licorice extract. The level of aflR gene expression was significantly decreased after the exposure of fungal cells to 500 g/mL of licorice extract. Evaluation of the antifungal and antitoxin activity of licorice extract on Aspergillus parasiticus revealed its antifungal properties as well as its effective ability to decrease aflatoxin production.

show abstract

“…Analytical methods for the detection of OTA, alone or in combination with other mycotoxins, have been reviewed [7][8][9][10]. Immunoaffinity column (IAC) clean-up in combination with high-performance liquid chromatography (HPLC) with fluorescence detection (FLD) is the most widely used procedure for the determination of OTA in food and feed.…”

Section: Introductionmentioning

confidence: 99%

Analytical performances of a DNA-ligand system using time-resolved fluorescence for the determination of ochratoxin A in wheat

Girolamo

Le²,

Penner³

et al. 2012

Anal Bioanal Chem

View full text Add to dashboard Cite

The analytical performances of a novel DNA-ligand system using the time-resolved fluorescence (TRF) response of ochratoxin A (OTA)-terbium-DNA aptamer interaction were tested for the quantitative determination of OTA in wheat. Wheat was extracted with acetonitrile/water (60:40, v/v) followed by clean-up through affinity columns containing a DNA-aptamer-based oligosorbent. Then, OTA was detected by TRF spectroscopy after reaction with a terbium fluorescent solution containing the DNA-aptamer probe. The entire procedure was performed in less than 30 min, including sample preparation, and allowed analysis of several samples simultaneously with a 96-well microplate reader. The average recovery from samples spiked with 2.5-25 μg kg(-1) OTA was 77%, with a relative standard deviation lower than 6% and a quantification limit of 0.5 μg kg(-1). Comparative analyses of 29 naturally contaminated (up to 14 μg kg(-1)) wheat samples using the aptamer-affinity column/TRF method or the immunoaffinity column/high-performance liquid chromatography method showed good correlation (r = 0.985) in the range tested. The trueness of the aptamer-based method was additionally assessed by analysis of two quality control wheat materials for OTA. The DNA-ligand system is innovative, simple and rapid, and can be used to screen large quantities of samples for OTA contamination at levels below the EU regulatory limit with analytical performances satisfying EU criteria for method acceptability.

show abstract

Developments in mycotoxin analysis: an update for 2010-2011

Cited by 80 publications

References 203 publications

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples

Antitoxin Characteristic of Licorice Extract: The Inhibitory Effect on Aflatoxin Production in Aspergillus parasiticus

Analytical performances of a DNA-ligand system using time-resolved fluorescence for the determination of ochratoxin A in wheat

Contact Info

Product

Resources

About