Developmentally induced changes in the sclerotial proteome of Sclerotinia sclerotiorum

Liang, Yue; Rahman, Masudur; Strelkov, Stephen E.; Kav, Nat N. V.

doi:10.1016/j.funbio.2010.05.003

Cited by 25 publications

(22 citation statements)

References 50 publications

(48 reference statements)

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“…In particular, the expression level increased between the development and maturation stages (Figure 3). An earlier study also reported a similar finding during MS development in Sclerotinia sclerotiorum [37]. In the present study, maximum consumption of O 2 was observed at the MS initiation (SI) period (data not shown), demonstrating that O 2 − was continuously produced and the alteration occurred between the UD and SI period.…”

Section: Discussionsupporting

confidence: 85%

Comparative transcriptome analysis of microsclerotia development in Nomuraea rileyi

et al. 2013

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BackgroundNomuraea rileyi is used as an environmental-friendly biopesticide. However, mass production and commercialization of this organism are limited due to its fastidious growth and sporulation requirements. When cultured in amended medium, we found that N. rileyi could produce microsclerotia bodies, replacing conidiophores as the infectious agent. However, little is known about the genes involved in microsclerotia development. In the present study, the transcriptomes were analyzed using next-generation sequencing technology to find the genes involved in microsclerotia development.ResultsA total of 4.69 Gb of clean nucleotides comprising 32,061 sequences was obtained, and 20,919 sequences were annotated (about 65%). Among the annotated sequences, only 5928 were annotated with 34 gene ontology (GO) functional categories, and 12,778 sequences were mapped to 165 pathways by searching against the Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) database. Furthermore, we assessed the transcriptomic differences between cultures grown in minimal and amended medium. In total, 4808 sequences were found to be differentially expressed; 719 differentially expressed unigenes were assigned to 25 GO classes and 1888 differentially expressed unigenes were assigned to 161 KEGG pathways, including 25 enrichment pathways. Subsequently, we examined the up-regulation or uniquely expressed genes following amended medium treatment, which were also expressed on the enrichment pathway, and found that most of them participated in mediating oxidative stress homeostasis. To elucidate the role of oxidative stress in microsclerotia development, we analyzed the diversification of unigenes using quantitative reverse transcription-PCR (RT-qPCR).ConclusionOur findings suggest that oxidative stress occurs during microsclerotia development, along with a broad metabolic activity change. Our data provide the most comprehensive sequence resource available for the study of N. rileyi. We believe that the transcriptome datasets will serve as an important public information platform to accelerate studies on N. rileyi microsclerotia.

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Section: Discussionsupporting

confidence: 85%

Comparative transcriptome analysis of microsclerotia development in Nomuraea rileyi

et al. 2013

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“…Two proteins with the GenBank accession numbers SS1G_13314 and SS1G_13315 have been identified (Liang et al, 2010). The deduced amino acid sequences of these two proteins are annotated in GenBank with the hypothetical functions of scytalone dehydratase and trihydroxynaphthalene reductase (1,3,8naphthalenetriol reductase), respectively.…”

Section: Sequence and Phylogenetic Analysismentioning

confidence: 99%

“…Some genes and proteins involved in sclerotial development in S. sclerotiorum have been identified and functionally characterized Rollins, 2009, 2010;Liang et al, 2010;Russo et al, 1982). Among them, two melanin biosynthesis-related proteins have been found to be up-regulated between the initiation and developmental stages of sclerotium formation, and the abundance of these two proteins is maintained at high levels after the initiation stage (Liang et al, 2010). As the effect of sclerotial melanin on the biology and pathogenicity of S. sclerotiorum is barely understood, in this study, we investigated the biological and pathogenicity function of the genes encoding these two proteins through a target gene replacement approach.…”

Section: Introductionmentioning

confidence: 99%

Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum

Liang

Wang

Steinkellner

et al. 2018

Molecular Plant Pathology

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The fungus Sclerotinia sclerotiorum is a necrotrophic plant pathogen causing significant damage on a broad range of crops. This fungus produces sclerotia that serve as the long-term survival structures in the life cycle and the primary inoculum in the disease cycle. Melanin plays an important role in protecting mycelia and sclerotia from ultraviolet radiation and other adverse environmental conditions. In this study, two genes, SCD1 encoding a scytalone dehydratase and THR1 encoding a trihydroxynaphthalene reductase, were disrupted by target gene replacement, and their roles in mycelial growth, sclerotial development and fungal pathogenicity were investigated. Phylogenetic analyses indicated that the deduced amino acid sequences of SCD1 and THR1 were similar to the orthologues of Botrytis cinerea. Expression of SCD1 was at higher levels in sclerotia relative to mycelia. THR1 was expressed at similar levels in mycelia and sclerotia at early stages, but was up-regulated in sclerotia at the maturation stage. Disruption of SCD1 or THR1 did not change the pathogenicity of the fungus, but resulted in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to ultraviolet light.

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“…Expressed sequence tag (EST) analysis was used to identify genes associated with pathogenesis by comparing the transcriptome of S. sclerotiorum grown on artificial medium to that during infection of Brassica napus [33]. A similar approach was used to identify genes expressed during different stages of S. sclerotiorum development on this host [34], which was later supported by proteomics analysis [35]. Subsequently, microarray [36] and RNA-Seq analysis [37] was used to explore the B. napus responses to S. sclerotiorum .…”

Section: Introductionmentioning

confidence: 99%

Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

et al. 2017

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Background Sclerotinia sclerotiorum causes stem rot in Brassica napus, which leads to lodging and severe yield losses. Although recent studies have explored significant progress in the characterization of individual S. sclerotiorum pathogenicity factors, a gap exists in profiling gene expression throughout the course of S. sclerotiorum infection on a host plant. In this study, RNA-Seq analysis was performed with focus on the events occurring through the early (1 h) to the middle (48 h) stages of infection.ResultsTranscript analysis revealed the temporal pattern and amplitude of the deployment of genes associated with aspects of pathogenicity or virulence during the course of S. sclerotiorum infection on Brassica napus. These genes were categorized into eight functional groups: hydrolytic enzymes, secondary metabolites, detoxification, signaling, development, secreted effectors, oxalic acid and reactive oxygen species production. The induction patterns of nearly all of these genes agreed with their predicted functions. Principal component analysis delineated gene expression patterns that signified transitions between pathogenic phases, namely host penetration, ramification and necrotic stages, and provided evidence for the occurrence of a brief biotrophic phase soon after host penetration.ConclusionsThe current observations support the notion that S. sclerotiorum deploys an array of factors and complex strategies to facilitate host colonization and mitigate host defenses. This investigation provides a broad overview of the sequential expression of virulence/pathogenicity-associated genes during infection of B. napus by S. sclerotiorum and provides information for further characterization of genes involved in the S. sclerotiorum-host plant interactions.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3642-5) contains supplementary material, which is available to authorized users.

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Developmentally induced changes in the sclerotial proteome of Sclerotinia sclerotiorum

Cited by 25 publications

References 50 publications

Comparative transcriptome analysis of microsclerotia development in Nomuraea rileyi

Comparative transcriptome analysis of microsclerotia development in Nomuraea rileyi

Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum

Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

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