Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum

Liang, Yue; Wang, Xiong; Steinkellner, Siegrid; Feng, Jie

doi:10.1111/mpp.12627

Cited by 54 publications

(35 citation statements)

References 36 publications

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“…The SM biosynthesis gene expressed at the highest level (LogFC = 7.6-9.2) was a gene encoding the PKS responsible for dihydroxy naphthalene (DHN) melanin biosynthesis (PKS13; sscle_03g031520) at 6-12 hpi as compared to the in vitro control, indicating a possible role in penetration during chickpea infection (Additional le: Table S5). In a previous study, disruption of S. sclerotiorum genes involved in melanin biosynthesis showed no change in pathogenicity; however, slower development of mycelial and hyphal branching was observed [32]. The current results indicate the importance of melanin to aid appressoria mediated penetration of S. sclerotiorum.…”

Section: S Sclerotiorum Secondary Metabolite Synthesis and Detoxi Casupporting

confidence: 55%

Analysis of Differentially Expressed Sclerotinia Sclerotiorum Genes During the Interaction With Moderately Resistant and Highly Susceptible Chickpea Lines

Mwape

Mobegi

Regmi

et al. 2021

Preprint

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Background: Sclerotinia sclerotiorum, the cause of Sclerotinia stem rot (SSR), is a host generalist necrotrophic fungus that can cause major yield losses in chickpea (Cicer arietinum) production. This study used RNA sequencing to conduct a timecourse transcriptional analysis of S. sclerotiorum gene expression during chickpea infection. The study explored S. sclerotiorum pathogenicity and developmental factors employed during chickpea infection. Results: During infection of moderately resistant and highly susceptible chickpea lines, 9,491 and 10,487 S. sclerotiorum genes, respectively, were significantly differentially expressed relative to in vitro. Analysis of the upregulated genes revealed enrichment of Gene Ontology biological processes, such as oxidation-reduction process, metabolic process, carbohydrate metabolic process, response to stimulus, and signal transduction. Several gene functional categories were upregulated in planta, including carbohydrate-active enzymes, secondary metabolite biosynthesis clusters, transcription factors and candidate secreted effectors. Differences in S. sclerotiorum genes expressed on varieties with different levels of susceptibility were also observed. Conclusion: These findings provide a framework for a better understanding of S. sclerotiorum interactions with hosts of varying susceptibility levels. Here, we report for the first time on the S. sclerotiorum transcriptome during chickpea infection, which could be important for further studies on this pathogen's molecular biology.

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Section: S Sclerotiorum Secondary Metabolite Synthesis and Detoxi Casupporting

confidence: 55%

Analysis of Differentially Expressed Sclerotinia Sclerotiorum Genes During the Interaction With Moderately Resistant and Highly Susceptible Chickpea Lines

Mwape

Mobegi

Regmi

et al. 2021

Preprint

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“…The most marked result of this analysis was the up-regulation of genes related secondary metabolism, particularly melanin. So far, only Scd1 (Sscle03g031470), Thr1 (Sscle03g031480), and Sspks13 (Sscle03g031520) have been characterized as genes pivotal in melanin biosynthesis within S. sclerotiorum [75], although a tetrahydroxynaphthalene reductase (Sscle09g070740) also contains homology to known components of this pathway in other fungi. All four of these genes were upregulated in the R infection at 24 hpi with logFC = ~ 2.0–2.4.…”

Section: Resultsmentioning

confidence: 99%

Gene regulation of Sclerotinia sclerotiorum during infection of Glycine max: on the road to pathogenesis

et al. 2019

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Background Sclerotinia sclerotiorum is a broad-host range necrotrophic pathogen which is the causative agent of Sclerotinia stem rot (SSR), and a major disease of soybean ( Glycine max ). A time course transcriptomic analysis was performed in both compatible and incompatible soybean lines to identify pathogenicity and developmental factors utilized by S. sclerotiorum to achieve pathogenic success. Results A comparison of genes expressed during early infection identified the potential importance of toxin efflux and nitrogen metabolism during the early stages of disease establishment. The later stages of infection were characterized by an apparent shift to survival structure formation. Analysis of genes highly upregulated in-planta revealed a temporal regulation of hydrolytic and detoxification enzymes, putative secreted effectors, and secondary metabolite synthesis genes. Redox regulation also appears to play a key role during the course of infection, as suggested by the high expression of genes involved in reactive oxygen species production and scavenging. Finally, distinct differences in early gene expression were noted based on the comparison of S. sclerotiorum infection of resistant and susceptible soybean lines. Conclusions Although many potential virulence factors have been noted in the S. sclerotiorum pathosystem, this study serves to highlight soybean specific processes most likely to be critical in successful infection. Functional studies of genes identified in this work are needed to confirm their importance to disease development, and may constitute valuable targets of RNAi approaches to improve resistance to SSR. Electronic supplementary material The online version of this article (10.1186/s12864-019-5517-4) contains supplementary material, which is available to authorized users.

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“…The DEPs were categorized into six groups: carbohydrate metabolism (three spots), protein synthesis (four spots), protein folding (five spots), proteolysis (three spots), signal transduction (one spot) and unknown function (five spots). Scytalone dehydratase has recently been reported to affect sclerotial development and vegetative growth in a necrotrophic plant pathogen, Sclerotinia sclerotiorum . In the present study, scytalone dehydratase (C17) was induced by oligochitosan, which may contribute to the inhibition of B. cinerea growth.…”

Section: Resultsmentioning

confidence: 50%

Proteomic analysis of the inhibitory effect of oligochitosan on the fungal pathogen, Botrytis cinerea

Meng

2018

J Sci Food Agric

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BACKGROUND: The fungal pathogen Botrytis cinerea infects a broad range of horticultural plants worldwide, resulting in significant economic losses. A derivative of chitosan, oligochitosan, has been reported to be an eco-friendly alternative to synthetic fungicides. RESULTS:Oligochitosan can greatly inhibit B. cinerea spore germination and induce protein carbonylation. To further investigate the molecular mechanism underlying the inhibitory effect, a comparative proteome analysis was conducted of oligochitosan-treated versus non-treated B. cinerea spores. The cellular proteins were obtained from B. cinerea spore samples and subjected to two-dimensional gel electrophoresis. In total, 21 differentially expressed proteins (DEPs) were identified. Three DEPs were up-regulated in the oligochitosan-treated versus the untreated spores, including scytalone dehydratase and a serine carboxypeptidase III precursor. By contrast, seven DEPs, including Hsp 88 and cell division cycle protein 48, were down-regulated by oligochitosan treatment. Notably, 10 DEPs, including phosphatidylserine decarboxylase proenzyme and ATP-dependent molecular chaperone HSC82, were only detected in the control spores, whereas one DEP, a non-annotated predicted protein, was only detected in the oligochitosan-treated spores. CONCLUSION: Oligochitosan may affect the spore germination of B. cinerea by impairing protein function. These findings have practical implications with respect to the use of oligochitosan for controlling fungal pathogens. A, Potential applications and antifungal activities of engineered nanomaterials against gray J Sci Food Agric 2019; 99: 2622-2628

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Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum

Cited by 54 publications

References 36 publications

Analysis of Differentially Expressed Sclerotinia Sclerotiorum Genes During the Interaction With Moderately Resistant and Highly Susceptible Chickpea Lines

Analysis of Differentially Expressed Sclerotinia Sclerotiorum Genes During the Interaction With Moderately Resistant and Highly Susceptible Chickpea Lines

Gene regulation of Sclerotinia sclerotiorum during infection of Glycine max: on the road to pathogenesis

Proteomic analysis of the inhibitory effect of oligochitosan on the fungal pathogen, Botrytis cinerea

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