Developmental toxicity of 3,4-dichloroaniline on rare minnow (Gobiocypris rarus) embryos and larvae

Zhu, Bin; Liu, Tianqiang; Hu, Xuegang; Wang, Gao‐Xue

doi:10.1016/j.chemosphere.2012.09.021

Cited by 45 publications

(31 citation statements)

References 28 publications

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“…Five replicates were set for the tests, with 200 embryos per replicate to behavioral changes (Zhong et al 2005;Hermsen et al 2011b). Thus, enzyme activity and mRNA level also play a critical role in these evaluation processes (Robinson et al 2012;Zhu et al 2013Zhu et al , 2014. In this study, some relative enzyme activities and mRNA levels were chosen for better understanding of the potential effects and action mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Body weight (mg) was measured by AUW220D analytical balance (Shimadzu; Tokyo, Japan). The body length (mm) and heart rate (beats/min) were counted, photographed, and videoed with a digital camera (Nikon, Japan) fixed on a dissection microscope and measured from these digital images using Image Pro Plus software (Media Cybernetics, Bethesda, MD, USA) and recorded (Zhu et al 2013). All spontaneous movement, heart beat, and swimming speed recordings started after adaptation to 25±1°C for 5 min.…”

Section: Developmental Toxicity Assaysmentioning

confidence: 99%

“…Real-time (quantitative) RT-qPCR was performed using CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) and SYBR Premix Ex Taq II kit (Takara, Dalian, China). RT-qPCR primers for hsp70, mstn, mt, apaf1, vezf1, cyp1a, and β-actin gene were designed by previous studies (Liu et al 2008;Yang et al 2010;Zhu et al 2013) and listed in Table 1. The housekeeping gene β-actin was used as an internal standard Zhu et al 2013Zhu et al , 2014.…”

Section: Developmental Toxicity Assaysmentioning

confidence: 99%

“…RT-qPCR primers for hsp70, mstn, mt, apaf1, vezf1, cyp1a, and β-actin gene were designed by previous studies (Liu et al 2008;Yang et al 2010;Zhu et al 2013) and listed in Table 1. The housekeeping gene β-actin was used as an internal standard Zhu et al 2013Zhu et al , 2014. PCR was run in the following cycling conditions: initial denaturation at 95°C for 10 min, 40 cycles of 95°C for 10 s, 58°C for 10 s, and 72°C for 30 s. Relative expression was calculated by using a modified comparative cycle threshold (CT) method.…”

Section: Developmental Toxicity Assaysmentioning

confidence: 99%

“…The G. rarus embryo as a useful research model also has lots of advantages, such as small in size and transparent, easy to maintain, short development cycle, rapid embryogenesis (Zha et al 2007;Zhu et al 2011). Earlier studies have shown that G. rarus embryos are sensitive to heavy metals, xenoestrogens, and other aquatic pollutant (Qun-Fang et al 2002;Wang et al 2010;Zhu et al 2011Zhu et al , 2013.…”

Section: Introductionmentioning

confidence: 96%

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Triazole-induced toxicity in developing rare minnow (Gobiocypris rarus) embryos

Zhu

Liu

Gong

et al. 2014

Environ Sci Pollut Res

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Using rare minnow (Gobiocypris rarus) at early-life stages as experimental models, the developmental toxicity of five widely used triazole fungicides (myclobutanil, fluconazole, flusilazole, triflumizole, and epoxiconazole) were investigated following exposure to 1-15 mg/L for 72 h. Meanwhile, morphological parameters (body length, body weight, and heart rate), enzyme activities (superoxide dismutase (SOD), glutathione S-transferase (GST), adenosine triphosphatase (ATPase), and acetyl cholinesterase (AChE)), and mRNA levels (hsp70, mstn, mt, apaf1, vezf1, and cyp1a) were also recorded following exposure to 0.2, 1.0, and 5.0 mg/L for 72 h. Results indicated that increased malformation and mortality, decreased body length, body weight, and heart rate provide a concentration-dependent pattern; values of 72 h LC50 (median lethal concentration) and EC50 (median effective concentration) ranged from 3 to 12 mg/L. Most importantly, the results of the present study suggest that even at the lowest concentration, 0.2 mg/L, five triazole fungicides also caused notable changes in enzyme activities and mRNA levels. Overall, the present study points out that those five triazole fungicides are highly toxic to the early development of G. rarus embryos. The information presented in this study will be helpful in better understanding the toxicity induced by triazole fungicides in fish embryos.

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Section: Discussionmentioning

confidence: 99%

Section: Developmental Toxicity Assaysmentioning

confidence: 99%

Section: Developmental Toxicity Assaysmentioning

confidence: 99%

Section: Developmental Toxicity Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Triazole-induced toxicity in developing rare minnow (Gobiocypris rarus) embryos

Zhu

Liu

Gong

et al. 2014

Environ Sci Pollut Res

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Development of cardiovascular and neurodevelopmental metrics as sublethal endpoints for the Fish embryo toxicity test

Krzykwa

Olivas

Jeffries

2018

Enviro Toxic and Chemistry

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The fathead minnow fish embryo toxicity (FET) test has been proposed as a more humane alternative to current toxicity testing methods as younger organisms are thought to experience less distress during toxicant exposure. However, the FET test protocol does not include endpoints that allow for the prediction of sublethal adverse outcomes, limiting its utility relative to other test types. Researchers have proposed the development of sublethal endpoints for the FET test to increase its utility. The present study 1) developed methods for previously unmeasured sublethal metrics in fathead minnows (i.e., spontaneous contraction frequency and heart rate) and 2) investigated the responsiveness of several sublethal endpoints related to growth (wet wt, length, and growth-related gene expression), neurodevelopment (spontaneous contraction frequency, eye size, and neurodevelopmental gene expression), and cardiovascular function and development (pericardial area, heart rate, and cardiovascular system-related gene expression) as additional FET test metrics using the model toxicant 3,4-dichloroaniline. Of the growth, neurological, and cardiovascular endpoints measured, length, eye size, and pericardial area were found to be more responsive than the other endpoints evaluated. Future studies linking alterations in these endpoints to longer-term adverse impacts are needed to fully evaluate the predictive power of these metrics in chemical and whole-effluent toxicity testing. Environ Toxicol Chem 2018;37:2530-2541. © 2018 SETAC.

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Chemicals Weaken Shoal Preference in the Rare MinnowGobiocypris rarus

Qiu

Wang

et al. 2020

Enviro Toxic and Chemistry

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Fish behavioral responses are sensitive to chemicals in the water. We tested rare minnow tested for their shoal preference, and the shoal (school) factors including nutritional status, body size, and shoal (school) size that can make their preference most stable were measured. Then shoal preference was measured again while fish and shoal were subjected to a concentration gradient of chemicals (cadmium ion [Cd 2+ ], tricaine methanesulfonate [MS222], and p-chloroaniline). The results showed that single rare minnow preferred shoals over blank control tanks. In addition, this preference was most stable when the shoal was well fed and contained 20 individuals 2 cm long. Although there was no significant response after exposure to p-chloroaniline, the time spent from entering the tank to start moving decreased greatly at concentrations of Cd 2+ >3 mg/L and MS222 >11 mg/L. The time the test fish spent close to the shoal significantly decreased at Cd 2+ >3 mg/L, MS222 >11 mg/L, and p-chloroaniline >10 mg/L, and the frequency of boundary line crossing increased at the same concentrations. The behavioral parameters changed by 20, 5, and 8 min once the lowest-observed-effect concentrations of Cd 2+ , MS222, and p-chloroaniline, respectively, were added. Our study provides useful information on rare minnow shoal preference that may be used for a biological early warning system.

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Developmental toxicity of 3,4-dichloroaniline on rare minnow (Gobiocypris rarus) embryos and larvae

Cited by 45 publications

References 28 publications

Triazole-induced toxicity in developing rare minnow (Gobiocypris rarus) embryos

Triazole-induced toxicity in developing rare minnow (Gobiocypris rarus) embryos

Development of cardiovascular and neurodevelopmental metrics as sublethal endpoints for the Fish embryo toxicity test

Chemicals Weaken Shoal Preference in the Rare MinnowGobiocypris rarus

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