DEVELOPMENTAL STUDIES IN PORPHYRA. I. BLADE DIFFERENTIATION IN PORPHYRA PERFORATA AS EXPRESSED BY MORPHOLOGY, ENZYMATIC DIGESTION, AND PROTOPLAST REGENERATION¹

Abstract

Four areas containing different cell morphologies were mapped on Porphyra blades and five different cell types (i.e. tapered with long extensions, large and vacuolated, vegetative and dividing, and reproductive: males and females) were identified in them. Tissues from these areas were dissociated, and protoplasts and single cells were isolated from the dissociated tissue of each distinct region. Regeneration rates of the isolated cells and protoplasts (isolates) varied depending on their morphological type. Regeneration rates were lowest in cultured isolates from the area just above the holdfast (ca. 1 %) and increased gradually to over 80% in isolates from areas of vegetative and reproductive regions away from the holdfast. Four distinct morphological patterns were observed among the regenerating plants. Cells isolated from vegetative areas developed into leafy plants while in liquid culture, and into calli when grown on solid medium. Isolates from reproductive areas developed into either a long thin or short thick filamentous plant. Those from ripe patches of carposporangia developed into thin conchocelis filaments, while isolates from non‐differentiated cells bordering the ripe reproductive patches developed into thick filaments resembling the morphology of conchosporangial branches. The blade of Porphyra appears simple as it consists of a single cell layer; however, it is complex both morphologically and physiologically.

Regeneration Studies in Grateloupia filicina (J.V. Lamouroux) C. Agardh - An Important Carrageenophyte and Edible Seaweed

“…Out of this 1 ml of IKI solution was added to 250 ml of sterilized seawater). Further sterilization was done by using broad-spectrum antibiotic mixture and 1% GeO 2 for 2 days to prevent the growth of bacteria and diatoms (Polne-Fuller and Gibor 1984).…”

REGENERASIRUMPUT LAUT Kappaphycus alvarezii (Doty) MELALUI INDUKSI KALUS DAN EMBRIO DENGAN PENAMBAHAN HORMON PERANGSANG TUMBUH SECARA IN VITRO

“…Fragmen yang dipilih untuk kultur jaringan, disterilkan dengan metode sterilisasi permukaan (Polne-Fuller & Gibor, 1984;Huang & Fujita, 1997). Explants dibersihkan dengan sikat di bawah mikroskop, kemudian dimasukkan ke dalam 0,5% deterjen cair dalam air laut steril selama 10 menit, kemudian dengan betadin 2% w/v) di dalam air laut steril selama 3 menit untuk menghilangkan mikroba permukaan, kemudian disterilisasi menggunakan campuran antibiotik 3% di dalam media kultur Conwy selama 2 hari.…”

Red and blue light regulation of growth and photosynthetic metabolism inPorphyra umbilicalis(Bangiales, Rhodophyta)

“…1. After this pretreatment, the experimental algae presented the characteristics of nitrogen limitation (L6pez- Figueroa & Niell, 1990), and their cells morphologically corresponded to group 3 II of Polne-Fuller & Gibor (1984). Discs of 9 mm diameter were cut from the thalli and treated briefly in Provasoli's enriched seawater (PES; Starr & Zeikus, 1987), to which antibiotics were added according to Robaina et al (1990) in order to prevent undesirable bacterial growth.…”