Developmental Sites of Neotropical Drosophilidae (Diptera): V. Inflorescences of Calathea Cylindrica andCalathea monophylla(Zingiberales: Marantaceae)

Abstract

In December 2006, 495 drosophilids belonging to 19 species were aspirated from inflorescences of Calathea monophylla (Vellozo) Körn at a forest reserve in the city of São Paulo, state of São Paulo, and 42 specimens belonging to three species of Drosophila were aspirated from those of Calathea cylindrica (Roscoe) Karl Schumann in an urban forest in the city of Rio de Janeiro, state of Rio de Janeiro, Brazil. In addition, 20 inflorescences of C. monophylla and 14 inflorescences of C.cylindrica were collected and observed for the emergence of flies in the laboratories. In total, 137 drosophilids belonging to Zygothrica dispar (Wiedemann, 1830) plus five species of Drosophila (Drosophila griseolineata Duda and four undescribed species) emerged from C. monophylla inflorescences, and a total of 22 specimens, all belonging to two undescribed species of Drosophila, emerged from those of C. cylindrica. Drosophila calatheae sp. nov., ungrouped but related to both the xanthopallescens Pipkin and the bromeliae Patterson & Stone species groups, is described based on both aspirated and emerged flies from C. monophylla inflorescences from São Paulo city. This new species was also reared in the laboratory with a new medium recipe, thus providing larvae for chromosomal studies.

The invasive spotted-wing Drosophila (Diptera, Drosophilidae) has been found in the city of São Paulo (Brazil)

“…Male (5, 6, 8-10) and female terminalia (7, 11) of Drosophila suzukii, isofemale line M50F1, São Paulo, state of São Paulo, Brazil. source software CombineZP as detailed by Vaz et al (2014). Voucher specimens will be housed in the Museu de Zoologia da Universidade de São Paulo.…”

The flower flies and the unknown diversity of Drosophilidae (Diptera): a biodiversity inventory in the Brazilian fauna

“…In fact, drosophilid records in Brazil are strongly biased towards fruit-feeding species of Drosophila, and to southeastern and southern localities (more exactly, near the main universities, and the present survey is no exception); moreover, about one third of the species recorded in Brazil is known for a single locality (Gottschalk et al, 2008). Some new anthophilic species were described recently for Neotropics (Vaz et al, 2014(Vaz et al, , 2018Grimaldi, 2016;Llangarí & Rafael, 2017;Vilela & Prieto, 2018), but none of them seem to be conspecific with the species of unknown identity reported in the present survey.…”

“…In Neotropics, the best-studied flower-specialized species belong to the Drosophila flavopilosa species group (Brncic, 1962a(Brncic, , 1966(Brncic, , 1978Santos & Vilela, 2005;Robe et al, 2013), associated to flowers of the genus Cestrum (Solanaceae). Other notable anthophilic Drosophila taxa in Neotropical region are the D. bromeliae and D. lutzii species groups, both widespread in the Neotropics and associated to flowers of several botanical families Silva & Martins, 2004;Grimaldi, 2016;Vilela & Prieto, 2018); the D. onychophora and D. xanthopallescens species groups, reported respectively to the northern Andes and Central America (Pipkin, 1964;Hunter, 1979), and some species of uncertain affinities associated to Araceae inflorescences Vaz et al, 2014Vaz et al, , 2018Llangarí & Rafael, 2017). Reports from other genera are also known, especially for Zygothrica Wiedemann (Grimaldi, 1987;Endara et al, 2010;Fonseca et al, 2017) and the poorly known Laccodrosophila Duda and Zapriothrica Wheeler (Heed et al, 1960).…”

Drosofilídeos (Diptera) associados a flores e fungos da mata atlântica: identificação de novas espécies e evolução do cromossomo Y

“…Para a visualização dos cromossomos mitóticos disseca-se larvas de 3 o estádio para separação dos gânglios cerebrais e preparação de acordo com o protocolo de Sullivan et al (2000). O método está detalhado em Vaz et al (2014;seção 4.1). Os objetivos da visualização de cromossomos mitóticos são: 1) determinar o cariótipo de espécies novas (como parte de sua descrição) e 2) confirmar o cariótipo X0 de espécies que não apresentam nenhum gene ligado ao Y nos testes de PCR (ver seção 3.5).…”

“…Calathea são usadas como substrato de desenvolvimento de duas novas espécies de Drosophila está descrita emVaz et al (2014; seção 4.1). Consiste basicamente em aspirar adultos sobre o substrato durante alguns dias seguidos e coletar as inflorescências, colocando-as em garrafas de plástico arejadas, com areia úmida no fundo para observar as imagos que emergem.…”