Developmental Profile of Enzyme Activity in Several Tissues
of the C57BL/6 Mouse between Birth and 35 Days of Age

Burkhart, James G.; Mohrenweiser, Harvey W.

doi:10.1159/000458586

Cited by 7 publications

(2 citation statements)

References 18 publications

(31 reference statements)

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“…These differences are also larger than would be expected simply by the reduced growth rate of the c3H/c 6H mice [34]. The enzyme developmental profile in the control mice is similar to that previously reported for C57BL/6 mice [35].…”

Section: Discussionsupporting

confidence: 86%

Enzyme changes associated with mitochondrial malic enzyme deficiency in mice

Mohrenweiser

Erickson

1979

Biochimica et Biophysica Acta (BBA) - General Subjects

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A genetically determined absence of mitochondrial malic enzyme (EC 1.1.1.40) in c3H/c6H mice is accompanied by a four-fold increase in liver glucose-6-phosphate dehydrogenase and a two-fold increase for 6-phosphogluconate dehydrogenase activity. Smaller increases in the activity of serine dehydratase and glutamic oxaloacetic transaminase are observed while the level of glutamic pyruvate transaminase activity is reduced in the liver of deficient mice. Unexpectedly, the level of activity of total malic enzyme in the livers of mitochondrial malic enzyme-deficient mice is increased approximately 50% compared to littermate controls. No similar increase in soluble malic enzyme activity is observed in heart of kidney tissue of mutant mice and the levels of total malic enzyme in these tissues are in accord with expected levels of activity in mitochondrial malic enzyme-deficient mice. The divergence in levels of enzyme activity between mutant and wild-type mice begins at 19--21 days of age. Immunoinactivation experiments with monospecific antisera to the soluble malic enzyme and glucose-6-phosphate dehydrogenase demonstrate that the activity increases represent increases in the amount of enzyme protein. The alterations are not consistent with a single hormonal response.

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Section: Discussionsupporting

confidence: 86%

Enzyme changes associated with mitochondrial malic enzyme deficiency in mice

Mohrenweiser

Erickson

1979

Biochimica et Biophysica Acta (BBA) - General Subjects

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“…The animals in experiment one were 12 weeks of age when sacrificed, while the animals in experiments two and three were 7 weeks of age. I t has been suggested that some of the differences seen in absolute activities might be due to age (Burkhart et al, 1978;Knox, 1972), but this was 24 Fig. 4.…”

Section: Discussionmentioning

confidence: 98%

Circadian organization of thirteen liver and six brain enzymes of the mouse

et al. 1981

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The activities of 13 liver and 6 brain enzymes were studied in 7-12 week old CD2F1 male mice that had been fed ad libitum and standardized either to 12 hours of light (0600-1800) alternating with 12 hours of darkness (1800-0600) (LD12:12); or to a reversed light-dark cycle (darkness 0600-1800; light 1800-0600) (DL12:12). Three separate studies were performed on two different days; in each experiment, subgroups of 14 animals were sacrificed at 3-hour intervals. Livers were assayed for: isocitrate dehydrogenase, glutamate dehydrogenase, lactate dehydrogenase, alcohol dehydrogenase, glutathione reductase, glyoxylate reductase, L-alanine aminotransferase, glutamate oxalacetate transaminase, pyruvate decarboxylase, fructose-1-phosphate aldolase, fructose diphosphate aldolase, fructose 1,6-diphosphatase, and fatty acid synthetase. Brains were assayed for phosphoglucose isomerase, adenosine triphosphatase, creatine phosphokinase, pyruvate kinase, adenylate kinase, and malate dehydrogenase. All 19 enzymes demonstrated a prominent circadian rhythm in at least one experiment. Moreover, each rhythmic variable showed a statistically significant fit to a 24-hour cosine (sine) curve by the method of least squares. In general, peak activities of the liver enzymes analyzed were associated with the beginning of the dark cycle and initiation of the animal's activity, while the group of brain enzymes had peak activities which occurred at the beginning of the animals' rest span and were near the beginning of the light cycle. The phasing of each of the rhythms could be reversed within a two-week span after reversing the environmental light-dark cycle 180 degrees.

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Dystrophic mutation (dy2J) affecting regulation of lactate dehydrogenase (LDH) and pyruvate kinase (PK) in C57BL/6J mice

1982

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The genotype difference (dystrophic vs nondystrophic) in the LDH isozymes is observed in kidney. These differences are evident only at birth and at early developmental stages (before the expression of dystrophic symptoms). The tissue specific genotype differences for PK are limited to the thigh muscle (M form) and heart (L form), after the onset of the condition. These differences may reflect the pleiotropic effect of the dy2J locus during the temporal regulation of these and other enzymes implicated in muscular dystrophy (MD).

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Developmental Profile of Enzyme Activity in Several Tissues of the C57BL/6 Mouse between Birth and 35 Days of Age

Cited by 7 publications

References 18 publications

Enzyme changes associated with mitochondrial malic enzyme deficiency in mice

Enzyme changes associated with mitochondrial malic enzyme deficiency in mice

Circadian organization of thirteen liver and six brain enzymes of the mouse

Dystrophic mutation (dy2J) affecting regulation of lactate dehydrogenase (LDH) and pyruvate kinase (PK) in C57BL/6J mice

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