Developmental Priming of Stomatal Sensitivity to Abscisic Acid by Leaf Microclimate

Pantin, Florent; Renaud, Jeanne; Barbier, François; Vavasseur, Alain; Thiec, Didier Le; Rose, Christophe; Bariac, Thierry; Casson, Stuart A.; McLachlan, Deirdre H.; Hetherington, Alistair M.; Muller, Bertrand; Simonneau, Thierry

doi:10.1016/j.cub.2013.07.050

Cited by 85 publications

(87 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown that stomatal responsiveness may be conditional on growth conditions (e.g. plants grown under very high humidity show reduced stomatal responsiveness to ABA; Pospíšilová, 1996;Pantin et al, 2013b;Arve et al, 2014). However, different growth conditions seem to be insufficient to explain the abovementioned differences between our results and those of McAdam et al ( , 2016b, even though different humidity regimes were used in our experiments.…”

Section: Strong Vpd Response Of Aba-deficient Mutantscontrasting

confidence: 55%

Stomatal VPD Response: There Is More to the Story Than ABA

et al. 2017

View full text Add to dashboard Cite

Guard cells shrink and close stomatal pores when air humidity decreases (i.e. when the difference between the vapor pressures of leaf and atmosphere [VPD] increases). The role of abscisic acid (ABA) in VPD-induced stomatal closure has been studied using ABA-related mutants that respond to VPD in some studies and not in others. The importance of ABA biosynthesis in guard cells versus vasculature for whole-plant stomatal regulation is unclear as well. Here, we show that Arabidopsis (Arabidopsis thaliana) lines carrying mutations in different steps of ABA biosynthesis as well as pea (Pisum sativum) wilty and tomato (Solanum lycopersicum) flacca ABA-deficient mutants had higher stomatal conductance compared with wild-type plants. To characterize the role of ABA production in different cells, we generated transgenic plants where ABA biosynthesis was rescued in guard cells or phloem companion cells of an ABA-deficient mutant. In both cases, the whole-plant stomatal conductance, stunted growth phenotype, and leaf ABA level were restored to wild-type values, pointing to the redundancy of ABA sources and to the effectiveness of leaf ABA transport. All ABA-deficient lines closed their stomata rapidly and extensively in response to high VPD, whereas plants with mutated protein kinase OST1 showed stunted VPD-induced responses. Another strongly ABAinsensitive mutant, defective in the six ABA PYR/RCAR receptors, responded to changes in VPD in both directions strongly and symmetrically, indicating that its VPD-induced closure could be passive hydraulic. We discuss that both the VPD-induced passive hydraulic stomatal closure and the stomatal VPD regulation of ABA-deficient mutants may be conditional on the initial pretreatment stomatal conductance.

show abstract

Section: Strong Vpd Response Of Aba-deficient Mutantscontrasting

confidence: 55%

Stomatal VPD Response: There Is More to the Story Than ABA

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Exposure to low RH can enhance stomatal ABA responsiveness in young leaves that are constantly in a microenvironment with high RH (Pantin et al, 2013b). This indicates that reduced RH is required to prime stomatal ABA responses, potentially via increased ABA levels, as ABA application can restore stomatal ABA responsiveness (Pantin et al, 2013b) and results in normal stomatal development (Fanourakis et al, 2011) in plants grown at high RH. However, in these reports, the RH that led to reduced ABA sensitivity was very high: 90% or more.…”

Section: Resultsmentioning

confidence: 99%

“…High relative air humidity during growth has been associated with longer and more open stomata (Torre et al, 2003;Nejad and van Meeteren, 2005;Fanourakis et al, 2011;Aliniaeifard et al, 2014), lower levels of ABA (Zeevaart, 1974;Nejad and van Meeteren, 2007;Okamoto et al, 2009;Aliniaeifard et al, 2014), and decreased stomatal responsiveness to ABA (Pospíšilová, 1996;Fanourakis et al, 2013;Pantin et al, 2013b;Arve et al, 2014). Exposure to low RH can enhance stomatal ABA responsiveness in young leaves that are constantly in a microenvironment with high RH (Pantin et al, 2013b). This indicates that reduced RH is required to prime stomatal ABA responses, potentially via increased ABA levels, as ABA application can restore stomatal ABA responsiveness (Pantin et al, 2013b) and results in normal stomatal development (Fanourakis et al, 2011) in plants grown at high RH.…”

Section: Resultsmentioning

confidence: 99%

Fern Stomatal Responses to ABA and CO₂ Depend on Species and Growth Conditions

2017

View full text Add to dashboard Cite

, climate, and air humidity affect plant gas exchange that is controlled by stomata, small pores on plant leaves and stems formed by guard cells. Evolution has shaped the morphology and regulatory mechanisms governing stomatal movements to correspond to the needs of various land plant groups over the past 400 million years. Stomata close in response to the plant hormone abscisic acid (ABA), elevated CO 2 concentration, and reduced air humidity. Whether the active regulatory mechanisms that control stomatal closure in response to these stimuli are present already in mosses, the oldest plant group with stomata, or were acquired more recently in angiosperms remains controversial. It has been suggested that the stomata of the basal vascular plants, such as ferns and lycophytes, close solely hydropassively. On the other hand, active stomatal closure in response to ABA and CO 2 was found in several moss, lycophyte, and fern species. Here, we show that the stomata of two temperate fern species respond to ABA and CO 2 and that an active mechanism of stomatal regulation in response to reduced air humidity is present in some ferns. Importantly, fern stomatal responses depend on growth conditions. The data indicate that the stomatal behavior of ferns is more complex than anticipated before, and active stomatal regulation is present in some ferns and has possibly been lost in others. Further analysis that takes into account fern species, life history, evolutionary age, and growth conditions is required to gain insight into the evolution of land plant stomatal responses.

show abstract

“…In the rosette plant Arabidopsis, stomatal sensitivity to ABA is acquired during leaf development by exposure to an increasingly dryer atmosphere, while young immature leaves, which develop in the center of the rosette, do not close in response to ABA. 30 Taken together, during the process of leaf development, that means from immature to mature, from young to aging, its stomatal sensitivity to ABA initially increased, and then decreased again. The young mature leaves have the maximum stomatal sensitivity to ABA than both the young immature leaves and the senescing leaves.…”

Section: The Development Stage-specificities Of Pyr1/pyl/ Rcar Aba Rementioning

confidence: 99%

“…10,28 The different stomatal sensitivity to ABA between mature leaves and young leaves in Arabidopsis rosette is also due to their distinct surrounding microclimate. 30 It seems that exposure of a leaf for a long time (several days) to some environmental conditions generates a sort of memory in the guard cells that results in the loss of suitable responses of the stomata to closing stimuli, such as desiccation and ABA. 47 The involved secondary messengers in stomatal movement is also related with leaves water conditions or RH.…”

Section: Spatio-temporal Specificities Of Pyr1/pyl/rcar Aba Receptorsmentioning

confidence: 99%

The spatio-temporal specificity of PYR1/PYL/RCAR ABA receptors in response to developmental and environmental cues

Sun

Fan

2017

Plant Signaling & Behavior

View full text Add to dashboard Cite

From the different functions ABA exerted between the aboveground and belowground, seed and vegetative tissues, primary root and lateral root, stimulating stomatal closure and inhibiting stomatal opening, between young and senescence leaves in stomatal movement, among different cells in plasma membrane water permeability, we addressed the organ-, tissue-, cell-, physiological processes-, and development stage specificities of PYR1/PYL/RCAR ABA receptors. This specificity may reflect the spatio-temporal properties of water potentials as well as the endogenous ABA levels in detail context, which plus the various affinities among this receptor families, resulted in the specificity of the transcripts as well as genes functions. PYR1/PYL/RCAR ABA receptors may integrate the message of ABA resource (local signaling or long distance signaling) and concentration, thus fine-tuning ABA response to environmental-and developmental cues. It also evolutionally affording land plants sophisticated mechanism to survival adverse environments.

show abstract

Developmental Priming of Stomatal Sensitivity to Abscisic Acid by Leaf Microclimate

Cited by 85 publications

References 43 publications

Stomatal VPD Response: There Is More to the Story Than ABA

Stomatal VPD Response: There Is More to the Story Than ABA

Fern Stomatal Responses to ABA and CO₂ Depend on Species and Growth Conditions

The spatio-temporal specificity of PYR1/PYL/RCAR ABA receptors in response to developmental and environmental cues

Contact Info

Product

Resources

About

Developmental Priming of Stomatal Sensitivity to Abscisic Acid by Leaf Microclimate

Cited by 85 publications

References 43 publications

Stomatal VPD Response: There Is More to the Story Than ABA

Stomatal VPD Response: There Is More to the Story Than ABA

Fern Stomatal Responses to ABA and CO2 Depend on Species and Growth Conditions

The spatio-temporal specificity of PYR1/PYL/RCAR ABA receptors in response to developmental and environmental cues

Contact Info

Product

Resources

About

Fern Stomatal Responses to ABA and CO₂ Depend on Species and Growth Conditions