The spatio-temporal specificity of PYR1/PYL/RCAR ABA receptors in response to developmental and environmental cues

Sun, Shanhui; Fan, Wenqiang; Mu, Zixin

doi:10.1080/15592324.2016.1214793

Cited by 9 publications

(7 citation statements)

References 42 publications

(66 reference statements)

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“…Depending on the previous occurrence of accumulated age‐related changes, the leaf can integrate environmental cues, which, if adverse, will induce the onset of senescence. The role of the rise of ABA contents and ABA signalling changes during leaf senescence is less clear (Sun et al ., ; Zhao et al ., ). ABA is involved in both developmental processes and stress responses (Finkelstein, ).…”

Section: Introductionmentioning

confidence: 99%

ABA signalling manipulation suppresses senescence of a leafy vegetable stored at room temperature

Miret

Munné‐Bosch

Dijkwel

2017

Plant Biotechnology Journal

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SummaryPostharvest senescence and associated stresses limit the shelf life and nutritional value of vegetables. Improved understanding of these processes creates options for better management. After harvest, controlled exposure to abiotic stresses and/or exogenous phytohormones can enhance nutraceutical, organoleptic and commercial longevity traits. With leaf senescence, abscisic acid (ABA) contents progressively rise, but the actual biological functions of this hormone through senescence still need to be clarified. Postharvest senescence of detached green cabbage leaves (Brassica oleracea var. capitata) was characterized under cold (4 °C) and room temperature (25 °C) storage conditions. Hormonal profiling of regions of the leaf blade (apical, medial, basal) revealed a decrease in cytokinins contents during the first days under both conditions, while ABA only increased at 25 °C. Treatments with ABA and a partial agonist of ABA (pyrabactin) for 8 days did not lead to significant effects on water and pigment contents, but increased cell integrity and altered 1‐aminocyclopropane‐1‐carboxylic acid (ACC) and cytokinins contents. Transcriptome analysis showed transcriptional regulation of ABA, cytokinin and ethylene metabolism and signalling; proteasome components; senescence regulation; protection of chloroplast functionality and cell homeostasis; and suppression of defence responses (including glucosinolates and phenylpropanoids metabolism). It is concluded that increasing the concentration of ABA (or its partial agonist pyrabactin) from the start of postharvest suppresses senescence of stored leaves, changes the transcriptional regulation of glucosinolates metabolism and down‐regulates biotic stress defence mechanisms. These results suggest a potential for manipulating ABA signalling for improving postharvest quality of leafy vegetables stored at ambient temperature.

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Section: Introductionmentioning

confidence: 99%

ABA signalling manipulation suppresses senescence of a leafy vegetable stored at room temperature

Miret

Munné‐Bosch

Dijkwel

2017

Plant Biotechnology Journal

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“…In the future, the further characterization of the downstream signaling molecules of LbPP2Cs and LbSnRK2s, followed by exploration of the LbPYL–LbPP2C–LbSnRK2 combinations, will greatly advance our understanding of LbPYL–mediated ABA signaling in Lycium plants, as complicated combinations have been shown in Arabidopsis , maize, and tomato [ 9 , 43 , 44 , 45 , 46 ]. Moreover, establishing an efficient CRISPR/Cas9 gene editing system for L. barbarum will greatly accelerate the genetic improvement of L. barbarum for climate adaptation, such as LbPYL10 .…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of LbPYL10 in Heat Tolerance in Goji (Lycium barbarum)

Li,

Liu,

Chen

et al. 2024

Plants

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The characterization of the PYL/RCAR ABA receptors in a great deal of plant species has dramatically advanced the study of ABA functions involved in key physiological processes. However, the genes in this family are still unclear in Lycium (Goji) plants, one of the well–known economically, medicinally, and ecologically valuable fruit crops. In the present work, 12 homologs of Arabidopsis PYL/RCAR ABA receptors were first identified and characterized from Lycium (L.) barbarum (LbPYLs). The quantitative real–time PCR (qRT–PCR) analysis showed that these genes had clear tissue–specific expression patterns, and most of them were transcribed in the root with the largest amount. Among the three subfamilies, while the Group I and Group III members were down–regulated by extraneous ABA, the Group II members were up–regulated. At 42 °C, most transcripts showed a rapid and violent up–regulation response to higher temperature, especially members of Group II. One of the genes in the Group II members, LbPYL10, was further functionally validated by virus–induced gene silencing (VIGS) technology. LbPYL10 positively regulates heat stress tolerance in L. barbarum by alleviating chlorophyll degradation, thus maintaining chlorophyll stability. Integrating the endogenous ABA level increase following heat stress, it may be concluded that LbPYL–mediated ABA signaling plays a vital role in the thermotolerance of L. barbarum plants. Our results highlight the strong potential of LbPYL genes in breeding genetically modified L. barbarum crops that acclimate to climate change.

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“…RCAR2/PYL9 overexpression conferred drought resistance by promoting ABA-mediated leaf senescence ( Zhao et al., 2016 ). This suggests that RCARs have distinct functions, which are associated with different tissues, developmental stages, and specific environmental conditions ( Sun et al., 2017 ). However, non-redundant functionality of RCAR in an ABA-dependent and/or ABA-independent manner remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold Stress

Lim

Lee

2020

Front. Plant Sci.

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Arabidopsis thaliana has 14 abscisic acid (ABA) receptors-PYR1/PYLs/RCARs-which have diverse and redundant functions in ABA signaling; however, the precise role of these ABA receptors remains to be elucidated. Here, we report the functional characterization of RCAR5/PYL11 in response to cold stress. Expression of RCAR5 gene in dry seeds and leaves was ABA-dependent and ABA-independent, respectively. Under cold stress conditions, seed germination was negatively affected by the level of RCAR5 expression, which was dependent on ABA and was regulated by HAB1, OST1, and ABI5downstream components of RCAR5 in ABA signaling. Leaves of RCAR5overexpressing plants showed enhanced stomatal closure-independent of ABA-and high expression levels of cold, dehydration, and/or ABA-responsive genes compared to those of wild-type; these traits conferred enhanced freezing tolerance. Our data suggest that RCAR5 functions in response to cold stress by delaying seed germination and inducing rapid stomatal closure via ABA-dependent and ABA-independent pathways, respectively.

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The spatio-temporal specificity of PYR1/PYL/RCAR ABA receptors in response to developmental and environmental cues

Cited by 9 publications

References 42 publications

ABA signalling manipulation suppresses senescence of a leafy vegetable stored at room temperature

ABA signalling manipulation suppresses senescence of a leafy vegetable stored at room temperature

Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of LbPYL10 in Heat Tolerance in Goji (Lycium barbarum)

ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold Stress

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