Developmental plasticity of rat cerebellar cortex after cisplatin injury: Inhibitory synapses and differentiating Purkinje neurons

Pisu, Maria Bonaria; Roda, Elisa; Avella, Debora; Bernocchi, Graziella

doi:10.1016/j.neuroscience.2004.08.023

Cited by 24 publications

(49 citation statements)

References 36 publications

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“…Similarly, cisplatin also caused complete dissolution of the basophilic materials of the nuclei of Purkinje cells of the cerebellum of the rats showing eosinophilia. This agrees with the report of Pisu et al (2004Pisu et al ( , 2005) that cisplatin has negative effects on cerebellar cortex neurons, especially Purkinje cells. Although platinum drugs are reported to have poor penetration of the blood-brain barrier (Gregg et al, 1992), sufficient levels of cisplatin have been shown to cause toxicity in the brain (Gulec et al, 2013), which this study demonstrated.…”

supporting

confidence: 93%

“…By implication, this cisplatin-induced Purkinje cell alteration may lead to cerebellar injury which may cause gait, movement and posture impairments (Pisu et al, 2004). Similarly, the histological alterations caused by neuronal death as observed in the dentate gyrus neurons and those of the pyramidal cells of Cornu Ammonis3 might disrupt the smooth flow of neural information from the entorhinal cortex to the granular layer of dentate gyrus and further propagation of mossy fibres to the CA3 and CA1 subzones of the hippocampus as reported by Vivar and Praag (2013).…”

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confidence: 99%

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Tomato pomace powder ameliorated cisplatin-induced microanatomical alterations in brain of Wistar rats

Owoeye¹,

Onwuka²

2015

Int. J. Bio. Chem. Sci

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Cisplatin chemotherapy is associated with neurotoxicity. Oxidative stress has been associated with its pathogenesis. Tomato (Lycopersicon esculentum) pomace powder (TPP) may be a preventive agent by virtue of its known antioxidant property. The possible protective role of TPP against cisplatin-induced alteration of the microanatomy of rat brain was investigated. Thirty rats were divided equally into five groups: control, propylene glycol, TPP, cisplatin (10 mg/kg i.p.); TPP 50 mg/kg plus cisplatin (10 mg/kg i.p.). All administrations were oral by gastric gavage given for 24 days except cisplatin which was given intraperitoneally on days 10 and 17. Rats were euthanized on the 25 th day of treatment. Blood parameters checked, neurobehavioural tests done and brain tissue were examined with regards to micro-anatomical parameters. Cisplatin caused a significant (p<0.05) reduction in lymphocytes. Neurobehavioural results showed that cisplatin caused a reduction in line crossing, rearing and forelimb grip. Cisplatin caused histological alterations in the cerebellum, dentate gyrus and Cornu Ammonis3 (CA3). Co-treatment of cisplatin with TTP ameliorated these haematological, neurobehavioural and histological alterations. In conclusion, Lycopersicon esculentum (as tomato pomace powder) demonstrated neuroprotection against cisplatin-induced alteration of microanatomy of rat cerebellum, dentate gyrus and Cornu Ammonis3 of rat brain.

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supporting

confidence: 93%

mentioning

confidence: 99%

Tomato pomace powder ameliorated cisplatin-induced microanatomical alterations in brain of Wistar rats

Owoeye¹,

Onwuka²

2015

Int. J. Bio. Chem. Sci

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“…In this light, we propose that the abnormal development of Purkinje cell dendrites detected at PD10 and PD11 is likely due to a changed guiding influence of parallel fibers as already observed by Altman (1973) as also may occur after cytotoxic injury (Pisu et al, 2004(Pisu et al, , 2005. Changes in this relationship may result in the disturbed cerebellar cytoarchitecture.…”

Section: Defects Of Cerebellum Fissuressupporting

confidence: 67%

Postnatal Development of the Central Nervous System: Anomalies in the Formation of Cerebellum Fissures

Cerri¹,

Piccolini²,

Bernocchi³

2010

The Anatomical Record

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A natural defect in rat cerebellum postnatal development has been found in the fissura prima, consisting in various complex configurations of the cerebellar layers. We investigated the genesis of fissure malformations through immunoreactions for PCNA, GFAP, GABAA a6, and calbindin to label proliferating cells of the external granular layer (egl), radial glial fibers, mature granule cells, and Purkinje cells, respectively. Results on critical stages of rat postnatal development provided interesting evidences on how the malformation develops in fissures prima and secunda. Early (postnatal day 10) at the site of malformation, the Bergmann radial glia was often retracted and showed distortions and irregular running. The interruption of GFAP-positive radial glial fibers could fit in with the presence of clusters of PCNA-unlabeled cells in the sites of fusion of the egl; the clusters of cells are granule cells since their soma is labeled by GABAA a6. Moreover, an altered migration of granule cell precursors to the internal granular layer was evident which, in turn, affected Purkinje cell differentiation and the growth of their dendrites. In summary, the changed relationship among glial fiber morphology, granule cell migration, and Purkinje cell differentiation suggests how the Bergmann glial fibers have a basic role in the foliation process, being the driving physical force in directing migration of the granule cells at the base of fissure. Anat Rec, 293:492-501, 2010. V V C 2010 Wiley-Liss, Inc.

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“…GluR2-3 labelling is localised at postsynaptic level in both Purkinje neuron somata and dendrite branches, in granule cells and glomeruli in the IGL (Hafidi & Hillman 1997;Pisu et al 2003Pisu et al , 2004.…”

Section: Resultsmentioning

confidence: 99%

“…The GABA-synthesising enzyme (Houser & Esclapez 1994) represents a good marker for GABAergic function (Martin & Rimvall 1993;Sheikh et al 1999) and was used to mark the distribution of GABAergic neurons, such as Purkinje cells (Erlander & Tobin 1991;Martin & Barke 1998;Esclapez & Houser 1999;Sheikh et al 1999). In particular, GAD67 marks axons and neuronal cell bodies (Scherini et al 1992;Pisu et al 2004;Popp et al 2009). Our findings on GAD67 expression indicated a loss of labelling in the axons surrounding Purkinje cell somata, particularly in March after a long hibernation period, indicating functional alterations within this neuronal population.…”

Section: Neurotransmissionmentioning

confidence: 99%

Changes in the cerebellar cytoarchitecture of hibernating hedgehogErinaceus europaeusL. (Mammalia): an immunocytochemical approach

Roda

Bottone

Insolia

et al. 2017

The European Zoological Journal

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Hibernation is an amazing animal strategy to survive when the environmental temperature is very low and food resources are scarce. Successful hibernation requires a variety of complex biological adaptations, in which the brain plays a central regulatory role. Currently, little information is available regarding the morphology and functional activity of specific neurons within the cerebellar cytoarchitecture of hibernating animals. In the present study, we investigated the immunohistochemical expression of essential proteins in the cerebellum of a mammalian hibernator (i.e. hedgehog Erinaceus europaeus L.), focusing on (i) Purkinje neurons, the sole output cells of the cerebellar cortex; (ii) selected neurotransmitters involved in hibernation processes; (iii) intracellular calcium homeostasis, considering that calcium is also an important regulator of neurotransmission mechanisms; and (iv) cytoskeletal proteins, involved in maintenance of neuronal shape and axon calibre. Specifically, we studied in situ immunocytochemical changes during the torpor state of hibernation (November-March) versus the activity phase (AprilSeptember). We employed different selected markers, i.e. glutamic acid decarboxylase (GAD67) and postsynaptic glutamate ionotropic receptor GluR2-3, different calcium-binding proteins (i.e. calbindin, parvalbumin and calretinin) and cytoskeletal components (i.e. pNF-H and MAP2). In summary, our data in hibernating animals demonstrated: (i) downregulation of GAD67, indicating loss/changes of synaptic contacts on Purkinje somata and dendrites; (ii) GluR2-3 upregulation in Purkinje neurons, with a drastic decrease of calbindin expression; and (iii) decrease of normal mechanisms regulating intracellular calcium homeostasis. We also found a decrease/modification in the distribution of cytoskeletal proteins, particularly evident for pNF-H. Changes in the functional activity of Purkinje cells were accompanied by some morphological dendrite alterations, signs of degeneration in cell somata and flattened basket pinceaux at the Purkinje axon hillock. These findings confirm that hibernation makes heterothermic animals a valuable model to study physiological adaptations to adverse conditions, and also for understanding cellular and molecular mechanisms aimed at preserving mammalian organs from full degeneration and death.

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Developmental plasticity of rat cerebellar cortex after cisplatin injury: Inhibitory synapses and differentiating Purkinje neurons

Cited by 24 publications

References 36 publications

Tomato pomace powder ameliorated cisplatin-induced microanatomical alterations in brain of Wistar rats

Tomato pomace powder ameliorated cisplatin-induced microanatomical alterations in brain of Wistar rats

Postnatal Development of the Central Nervous System: Anomalies in the Formation of Cerebellum Fissures

Changes in the cerebellar cytoarchitecture of hibernating hedgehogErinaceus europaeusL. (Mammalia): an immunocytochemical approach

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