Developmental expression of cytochrome p450s within intrasplenically transplanted fetal hepatocytes

Katô, Kazuya; Kasai, S.; Onodera, Kenji; Sawa, Masayuki; Kawabata, Norihiro; Nakazawa, Fumiaki; Imai, Masato; Kino, Yasuhisa; Mito, Michio; John, W Erickson; Hodgson, Barbara B.

doi:10.1016/0963-6897(96)00035-8

Cited by 1 publication

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7 In animal studies, successful engraftment and function of transplanted hepatocytes were shown in many different sites and have been well documented over the past 20 years. 7,17,18,20 Spleen and liver favor long-term engraftment and function of donor hepato- cytes better than other sites studied. 7,20,24,25 The precise amount of hepatocytes required to sustain the minimal metabolic needs of the human recipient and the time for engraftment and function of these cells have not been determined.…”

Section: Discussionmentioning

confidence: 99%

“…15 After 24 hours, RNA from these hepatocytes was isolated to perform Northern blots. 16 Another aliquot of 2 ϫ 10 7 hepatocytes from each preparation was transplanted into the spleen of Fisher 344 rats 17 and maintained under cyclosporine A monoimmunosuppression. 18 Seven days posttransplantation, all animals were killed to harvest their livers and spleens for histological examination and RNA preparation to show the presence of inducibility of transplanted hepatocytes.…”

Section: Functional Assessment Of Cryopreserved Hepatocytesmentioning

confidence: 99%

“…18 Seven days posttransplantation, all animals were killed to harvest their livers and spleens for histological examination and RNA preparation to show the presence of inducibility of transplanted hepatocytes. 17,18 Results from these studies served as a quality control measure but did not influence clinical management.…”

Section: Functional Assessment Of Cryopreserved Hepatocytesmentioning

confidence: 99%

See 2 more Smart Citations

Hepatocyte transplantation in acute liver failure

et al. 2000

View full text Add to dashboard Cite

The majority of patients with acute liver failure (ALF) die waiting for orthotopic liver transplantation (OLT). No other treatment modality is shown to improve survival. This study was conducted to assess the safety and feasibility of hepatocyte transplantation (HT) and subsequent engraftment and function of donor cells. Functional and structural integrity of cryopreserved and thawed human hepatocytes were assessed by their morphological characteristics, induction of P-4501A1 transcription, and survival in vivo by xenotransplantation into rats. Five patients with severe ALF underwent intrasplenic (4 patients) and/or intrahepatic (2 patients) HT through angiography under cyclosporine immunosuppression. All patients had grade III to IV encephalopathy and factor V levels less than 0.5 U/mL, were ventilator and dialysis dependent, and were not OLT candidates. Three of the 5 patients who survived 48 hours after HT had substantial improvement in encephalopathy scores, arterial ammonia levels, and prothrombin times. Clinical improvement was paralleled by an increase in aminopyrine and caffeine clearances. All 3 patients lived substantially longer than expected based on clinical experience after HT (12, 28, and 52 days) but eventually died. Postmortem examination showed the presence of transplanted hepatocytes in liver and spleen by light microscopy and fluorescent in situ hybridization (FISH). Cryopreserved and thawed human hepatocytes can be transplanted into recipients with ALF with some acceptable but definite complications. Engraftment of donor hepatocytes was proven by histological examination and FISH by both transjugular biopsy and at autopsy. Improvement in brain edema, encephalopathy grade, and clearance of antipyrine and caffeine suggested function, albeit with a 24- to 72-hour delay posttransplantation.

show abstract