Developmental expression and alternative splicing of the duck myostatin gene

Huang, Kailiang; Wang, Jiwen; Han, Chunchun; Liu, Hehe; Li, Liang; Dai, Fei; Pan, Zhenxiao; Xu, Feng; He, Hua; Xu, Hengyong

doi:10.1016/j.cbd.2011.04.002

Cited by 13 publications

(14 citation statements)

References 21 publications

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“…A recent study showed that miR-133 directly down-regulated nPTB during muscle development and establishes a role for miRs in the control of a developmentally dynamic splicing program (Boutz et al, 2007). Interestingly, four alternatively spliced transcripts of MSTN mRNA are expressed during duck embryonic skeletal muscle development (Huang et al, 2011). Alternative splicing of MSTN was described also in developing chicken (Moon et al, 2005;Castelhano-Barbosa et al, 2005).…”

Section: Discussionmentioning

confidence: 94%

Structural and functional analysis of myostatin-2 promoter alleles from the marine fish Sparus aurata: Evidence for strong muscle-specific promoter activity and post-transcriptional regulation

Nadjar-Boger

Hinits

Funkenstein

2012

Molecular and Cellular Endocrinology

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Section: Discussionmentioning

confidence: 94%

Structural and functional analysis of myostatin-2 promoter alleles from the marine fish Sparus aurata: Evidence for strong muscle-specific promoter activity and post-transcriptional regulation

Nadjar-Boger

Hinits

Funkenstein

2012

Molecular and Cellular Endocrinology

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“…Among 5 MSTN isoforms (A to E forms) in the avian, MSTN-A and MSTN-B form are major and second dominant forms in the muscle, respectively ( Huang et al., 2011 , Shin et al., 2015 ). Our previous in vitro study showed that binding of MSTN-B to MSNT-A causes a reduced production of mature MSTN from the MSTN-A, resulting in increases in muscle fiber length, diameter, and nuclei numbers ( Shin et al., 2015 ).…”

Section: Resultsmentioning

confidence: 99%

Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines

et al. 2020

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Myostatin ( MSTN ) negatively regulates in muscle growth and development. Among alternative splicing isoforms of avian MSTN, MSTN-A has antimyogenic activities and MSTN-B functions as a promyogenic factor. In this study, different lines of Japanese quail were used: a random bred control ( RBC ) and a heavy weight ( HW ) quail line with muscle hypertrophy. The objectives of the current study are to compare temporal expression of the MSTN isoforms in pectoralis major muscle ( PM ) between 2 quail lines and to relate MSTN expression with temporal changes in muscle growth and total amounts of DNA in PM. Gains of body weight ( BW ) and PM weight were greater until posthatch day ( D ) 28 ( P < 0.001), and the fold increases in total DNA contents of PM were greater in the HW line compared with the RBC line during D7 to D28 ( P < 0.05). PCR analysis showed that MSTN-A expression was greater at 14 D (E14) of embryonic age ( P < 0.01), D7 ( P = 0.052), and D14 ( P < 0.01) in the RBC line compared with the HW line. At D28 and D75, expression of MSTN-A was greater in the HW line compared with the RBC line ( P < 0.05). MSTN -B expression was barely detectable from E14 to D14 and measurable from D28 to D75 in the muscle of both lines. Ratios of the MSTN -B/-A form ranging from 0.15 to 0.29 indicate a minor expression of the B form. Taken together, the lesser expression levels of MSTN -A at E14, D7, and D14 are associated with the fast growth of PM, and greater MSTN-A expression at D28 and D75 are associated with a slowdown of PM growth in the HW line. These data indicate a negative association of MSTN expression with PM growth and provide a scientific basis for potential usage of MSTN expression as a selection marker for greater muscle growth in poultry.

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“…Of the five MSTN isoforms produced by alternative splicing in avians, two isoforms, MSTN-A and MSTN-B, are highly expressed in skeletal muscle [18,19]. MSTN-A encodes the full-length peptide, which is a strong negative regulator of myoblast proliferation and differentiation [20].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, knockdown of ACVR2B by shRNAs increased body and muscle weights in chickens, confirming the importance of this receptor in the signaling cascade [17]. Multiple MSTN isoforms produced by alternative splicing have been revealed in avians (MSTN-A to MSTN-E) [18]. The full-length MSTN peptide, encoded by MSTN-A, and another isoform, MSTN-B, which encodes a truncated peptide devoid of the active C-terminal region, are highly expressed in skeletal muscle.…”

Section: Introductionmentioning

confidence: 92%

Exogenous Expression of an Alternative Splicing Variant of Myostatin Prompts Leg Muscle Fiber Hyperplasia in Japanese Quail

Chen

Suh

Shin

et al. 2019

IJMS

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Myostatin (MSTN) negatively regulates muscle growth and development through inhibiting myoblast proliferation and differentiation. Five alternative splicing isoforms of MSTN (MSTN-A to MSTN-E) have been discovered in domestic avian species. MSTN-A has high expression in skeletal muscle and encodes the full-length peptide with anti-myogenic activity. Another isoform, MSTN-B, is also highly expressed in skeletal muscle and encodes a truncated peptide that has pro-myogenic capabilities in vitro, which include promoting the proliferation and differentiation of quail muscle precursor cells. The objective of this study was to investigate overexpression of MSTN-B in vivo by using two independent lines of transgenic Japanese quail with expression directed in the skeletal muscle. Unexpectedly, the chicken skeletal muscle alpha actin 1 (cACTA1) promoter resulted in restricted exogenous MSTN-B protein expression to certain skeletal muscles, such as the gastrocnemius and tibialis anterior, but not the pectoralis major muscle. Gastrocnemius weight as a percentage of body weight in transgenic quail was increased compared to non-transgenic quail at posthatch day 21 (D21) and posthatch D42. An increase in the size of the gastrocnemius in transgenic quail was attributed to an increase in fiber number but not fiber cross-sectional area (CSA). During embryonic development, paired box 7 (PAX7) expression was prolonged in the transgenic embryos, but other myogenic regulatory factors (MRFs) were unchanged after MSTN-B overexpression. Taken together, these data provide novel insights into the regulation of skeletal muscle development by alternative splicing mechanisms in avians.

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Developmental expression and alternative splicing of the duck myostatin gene

Cited by 13 publications

References 21 publications

Structural and functional analysis of myostatin-2 promoter alleles from the marine fish Sparus aurata: Evidence for strong muscle-specific promoter activity and post-transcriptional regulation

Structural and functional analysis of myostatin-2 promoter alleles from the marine fish Sparus aurata: Evidence for strong muscle-specific promoter activity and post-transcriptional regulation

Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines

Exogenous Expression of an Alternative Splicing Variant of Myostatin Prompts Leg Muscle Fiber Hyperplasia in Japanese Quail

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