Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines

Kim, Dong Hwan; Choi, Young Min; Suh, Young Ho; Shin, Sangsu; Lee, Joonbum; Hwang, Seongsoo; Lee, Kichoon

doi:10.1016/j.psj.2019.12.069

Cited by 7 publications

(10 citation statements)

References 27 publications

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“…Heavy-weight (HW) and low-weight (LW) quail lines have been developed by selectively breeding of more than 80 generations for body weight [ 17 ]. The HW quail line showed muscle hypertrophy with a higher ratio of Mstn-B / Mstn-A forms, and the LW line showed hypoplasia with a lower ratio of Mstn-B / Mstn-A forms [ 8 , 18 ]. In addition, overexpression of the MSTN-B form in transgenic quail increased muscle mass [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of MSTN Isoforms in Muscle between Broiler and Layer Chickens

Kim

Choi

Lee

et al. 2022

Animals

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Myostatin (Mstn)-A, the main isoform among Mstn splicing variants, functions as a negative regulator, whereas Mstn-B functions as a positive regulator in muscle development. Because broiler chickens are a fast-growing breed raised for meat production and layer chickens are a slow-growing breed raised for egg production, differences in the expression of Mstn isoforms between the two distinct breeds were analyzed in this study. There was no difference in the expression levels of total Mstn (Mstn-A and -B forms) during embryonic development and at D33 between the two breeds. Interestingly, the ratios of Mstn-B to -A were significantly higher in the broiler compared to the layer at most ages. In pectoralis major muscle (PM) tissue, the cross-sectional area (CSA) of muscle fiber was significantly greater in the broiler. The broiler also showed greater bundle CSA and a similar fiber number per bundle compared to the layer at D5 and D33. These data suggest that the greater bundle CSA with myofiber hypertrophy in the broilers is associated with greater muscle growth. The relationship between the expression of Mstn isoforms and growth rate can be used as a potential genetic marker for the selection of higher muscle growth in chickens.

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Section: Discussionmentioning

confidence: 99%

Differential Expression of MSTN Isoforms in Muscle between Broiler and Layer Chickens

Kim

Choi

Lee

et al. 2022

Animals

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“…For example, myostatin is a negative regulator in muscle development, and animals with the myostatin mutation produce more muscle mass [ 4 ]. Currently, an effective breeding method is selecting myostatin-mutant individuals to propagate high-performance offspring in Bos taurus and Coturnix japonica [ 4 , 5 , 6 ]. During the selection of 11 generations for AFP (abdominal fat percentage), the fat chicken line and lean chicken line were produced from the same ancestors, and Wang et al found that the level of AFP was 5.29 times higher in the fat chicken line than in the lean one [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Untargeted Metabolomics Reveals the Effect of Selective Breeding on the Quality of Chicken Meat

et al. 2022

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The selection for improved body weight is an effective approach in animal breeding. Guangxi Partridge chickens have differentiated into two lines under selective breeding, which include line S and line D that have shown statistically significant differences in body weight. However, the meat quality analysis in our study indicated that the quality of breast and thigh muscles in line S chickens changed, which included increased values of L*, b*, and drip loss and decreased a* value, pH, and shear force in skeletal muscles. To illuminate the effect of selection on skeletal muscles, LC-MS/MS metabolomics was performed to explore differentiated metabolites in divergent tissues from the two chicken lines. The results of principal component analysis and orthogonal projection to latent structures discriminant analysis suggested that metabolites of different groups were separated, which suggested that selective breeding certainly affected metabolism of skeletal muscles. KEGG analysis identified that valine, leucine, and isoleucine biosynthesis, glycerophospholipid metabolism, and glutathione metabolism noteworthily changed in breast muscle. Amino sugars and nucleotide sugar metabolism, ascorbate and aldarate metabolism, the pentose phosphate pathway, pentose and glucuronate interconversions, fructose and mannose metabolism, and glycerophospholipid metabolism were remarkedly identified in thigh muscle. These screened pathways suggested oxidative stress in breast and thigh muscles, which corresponded with our previous results. Therefore, this study determined that glycerophospholipid metabolism conservatively functioned in muscle flavor and development but exhibited different anti-oxidative patterns in different skeletal muscles. Overall, the present study identified several differentiated metabolites and pathways for exploring differences in meat quality between different broiler populations.

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“…These findings suggest that differential expression of Mstn may potentially contribute to embryonic muscle development when the myofiber is being formed, thereby influencing rates of muscle growth at posthatch ages. This explanation is partially supported by the recent findings of the involvement of Mstn in regulation of total myofiber numbers in chickens and quail with CRISPR/Cas-mediated indel mutations or shRNA-mediated knockdown of the Mstn gene (Bhattacharya et al., 2019; Kim et al., 2020a ; Lee et al., 2020 ).…”

Section: Resultsmentioning

confidence: 72%

“…Among alternative mRNA splicing variants ( Mstn-A to Mstn-E ) in avian Mstn , Mstn-A , a dominant form, had an inhibitory effect on proliferation of quail myogenic cells, fusion rate, and myotube formation in vitro ( Shin et al., 2015 ). Our recent study showed that the heavy-weight quail line ( HW ) had a muscle hypertrophic phenotype with low expression levels of Mstn in E14 compared with the CON ( Kim et al., 2020a ). Opposite of the antimyogenic function of Mstn-A , the Mstn-B form has promyogenic function ( Shin et al., 2015 ).…”

Section: Resultsmentioning

confidence: 99%

“…Among the variants, exogenous overexpression of an alternative splicing variant, Mstn-B , in transgenic quail increased muscle weight with hyperplasia by inhibiting proteolytic cleavage of pro-MSTN ( Shin et al., 2015 ; Chen et al., 2019 ). Recently, differential expression of splicing variants of Mstn has been associated with hypertrophic muscle growth in a heavy weight ( HW ) quail line ( Kim et al., 2020a ). However, temporal expression of Mstn variants has not been investigated in a low-weight ( LW ) quail line with muscle hypoplasia ( Choi et al., 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Research Note: Increased myostatin expression and decreased expression of myogenic regulatory factors in embryonic ages in a quail line with muscle hypoplasia

et al. 2021

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Genetic selection of quail for a low body weight for more than 80 generations established a low-weight ( LW ) Japanese quail line that has been previously characterized to have a muscle hypoplasia phenotype. The aim of this study is to investigate the relationship of temporal expression levels of myostatin ( Mstn ) and myogenic regulatory factors ( MRFs ) with hypoplastic muscle growth in the LW line. During embryonic day ( E ) 13 to 15, gain of embryo weight was 2-fold lower ( P < 0.001) in the LW line than that in the random bred control ( CON ). Gains in body weight and pectoralis muscle weight from hatch to posthatch day ( P ) 28 were also significantly lower ( P < 0.01) in the LW line but increased by 4-fold ( P < 0.05) during P42 to P75. PCR analysis showed that expression levels of Mstn were greater in the LW at embryonic stage (E12 to E14, P < 0.05), but there was no difference after hatch. In addition, expression levels of Pax7 and myogenin ( MyoG ) at E12 were 23-fold ( P < 0.05) and 3.4-fold ( P < 0.05) lesser in the LW line, respectively. At E14, expression of Pax3 , Pax7 , and MyoG gene was 3.5-fold ( P < 0.05), 6.5-fold ( P = 0.065), and 4.4-fold ( P < 0.01) less than that in the CON. Taken together, high expression levels of Mstn and low expression of MRFs during embryonic stages can be associated with development of muscle hypoplasia and delayed muscle growth in the LW quail line. These data provide evidence that genetic selection for a low body weight resulting in an avian model with muscle hypoplasia has altered the expression profiles of myogenic factors.

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Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines

Cited by 7 publications

References 27 publications

Differential Expression of MSTN Isoforms in Muscle between Broiler and Layer Chickens

Differential Expression of MSTN Isoforms in Muscle between Broiler and Layer Chickens

Untargeted Metabolomics Reveals the Effect of Selective Breeding on the Quality of Chicken Meat

Research Note: Increased myostatin expression and decreased expression of myogenic regulatory factors in embryonic ages in a quail line with muscle hypoplasia

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