Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production

Machatkova, Marie; Krausova, K.; Jokešová, E.; Tománek, M.

doi:10.1016/s0093-691x(03)00216-4

Cited by 104 publications

(61 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Only good quality oocytes with homogenous dark cytoplasm and at least two layers of cumulus cells were considered usable. Embryos were prepared according to a standard protocol described previously (Machatkova et al, 2004). Oocytes were matured in TCM-199 medium (Earle's salt), supplemented with antibiotics, 0.20mM sodium pyruvate (Sigma-Aldrich, St. Louis, MO, USA), gonadotropins (P.G.…”

Section: Embryo Productionmentioning

confidence: 99%

“…Both the embryo quality and the kinetics of embryo development are related to the oocyte and, most importantly, to its meiotic and developmental competence (Rizos et al, 2002;Sirard et al, 2006;Lonergan, 2007). It has been documented that follicles present on the ovaries at the end of the growth phase of follicular wave can provide oocytes with greater meiotic and developmental competence (Hagemann et al, 1999;de Wit et al, 2000;Machatkova et al, 2004).…”

mentioning

confidence: 99%

See 1 more Smart Citation

In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

Machatkova¹,

Hulinska²,

Rečková³

et al. 2008

Vet. Med. - Czech

Self Cite

View full text Add to dashboard Cite

AbstrAct:In this field study, embryos were derived from genetically highly valuable cows excluded from breeding due to reproductive disorders. Cows, 5 to 10 years old, of Czech Siemmental, Holstein Dairy and Beef Cattle breeds were used as oocyte donors. Oocytes were obtained either in the growth phase of the first follicular wave from cows with synchronized oestrus or in any other phases of follicular development from cows without oestrus synchronization. The embryos were prepared by a standard protocol described previously. The mean number of usable oocytes, transferable and freezable embryos per donor, and the mean percentage of usable, transferable and freezable embryos were assessed. The results were analyzed by Student's-t and Chi-squared tests. The embryos were frozen according to a slow freezing protocol. After thawing, they were transferred to recipients on Day 7 after oestrus. Irrespective of the breed, the mean numbers of usable oocytes and transferable and freezable embryos collected per donor were significantly higher (P < 0.01) for the synchronized than for the nonsynchronized donors (20.4 vs 11.7, 4.3 vs 1.0 and 3.2 vs 0.8, respectively). Similarly, the mean percentages of usable oocytes, transferable and freezable embryos were significantly higher (P < 0.01) for the synchronized than for the nonsynchronized donors (28.5% vs 20.5%, 20.9% vs 9.0% and 15.8% vs 6.5%, respectively). On comparison of the synchronized and nonsynchronized donors of each breed, the difference in the mean percentage of usable oocytes was significant (P < 0.01) in cows of all three breeds, the difference in the mean percentage of transferable embryos was significant in Czech Siemmental and Holstein Dairy cows (P < 0.01) and the difference in the mean percentage of freezable embryos was significant only in Holstein Dairy cows (P < 0.01). After the transfer of 41 frozen-thawed embryos and 43 fresh embryos, 20 heifers and 24 heifers became pregnant, respectively. In conclusion: (a) higher number of oocytes from infertile, genetically valuable cows was recovered in the growth phase compared with the other phases of follicular development; (b) greater development of these oocytes resulted in more embryos for transfer and cryopreservation; (c) the transfer of frozen-thawed and fresh embryos resulted in pregnancy rates of 48.8% and 55.8% , respectively.

show abstract

Section: Embryo Productionmentioning

confidence: 99%

mentioning

confidence: 99%

In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

Machatkova¹,

Hulinska²,

Rečková³

et al. 2008

Vet. Med. - Czech

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many factors have been shown to affect the oocyte's developmental potential, including follicle size (Lonergan et al 1994), health of the follicle (Blondin & Sirard 1995, Vassena et al 2003, phase of follicular wave (Hagemann 1999, Machatková et al 2004, hormonal stimulation (Blondin et al 2002; for review Sirard et al 2006), maturation environment (Warzych et al 2007; for review Sutton et al 2003), season (Al-Katanani et al 2002, Sartori et al 2002, nutrition (Fouladi-Nashta et al 2007) and age (Rizos et al 2005). Although previous studies support the notion that oocyte competence depends on multiple factors, it remains difficult to draw clear and reliable criteria for oocyte selection.…”

Section: Introductionmentioning

confidence: 99%

Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity

Torner¹,

Ghanem²,

Ambros³

et al. 2008

Reproduction

View full text Add to dashboard Cite

Oocyte selection based on glucose-6-phosphate dehydrogenase (G6PDH) activity has been successfully used to differentiate between competent and incompetent bovine oocytes. However, the intrinsic molecular and subcellular characteristics of these oocytes have not yet been investigated. Here, we aim to identify molecular and functional markers associated with oocyte developmental potential when selected based on G6PDH activity. Immature compact cumulus-oocyte complexes were stained with brilliant cresyl blue (BCB) for 90 min. Based on their colouration, oocytes were divided into BCB K (colourless cytoplasm, high G6PDH activity) and BCB C (coloured cytoplasm, low G6PDH activity). The chromatin configuration of the nucleus and the mitochondrial activity of oocytes were determined by fluorescence labelling and photometric measurement. The abundance and phosphorylation pattern of protein kinases Akt and MAP were estimated by Western blot analysis. A bovine cDNA microarray was used to analyse the gene expression profiles of BCB C and BCB K oocytes. Consequently, marked differences were found in blastocyst rate at day 8 between BCB C (33.1G3.1%) and BCB K (12.1G1.5%) oocytes. Moreover, BCB C oocytes were found to show higher phosphorylation levels of Akt and MAP kinases and are enriched with genes regulating transcription (SMARCA5), cell cycle (nuclear autoantigenic sperm protein, NASP ) and protein biosynthesis (RPS274A and mRNA for elongation factor 1a, EF1A). BCB K oocytes, which revealed higher mitochondrial activity and still nucleoli in their germinal vesicles, were enriched with genes involved in ATP synthesis (ATP5A1), mitochondrial electron transport (FL405), calcium ion binding (S100A10) and growth factor activity (bone morphogenetic protein 15, BMP15). This study has evidenced molecular and subcellular organisational differences of oocytes with different G6PDH activity.

show abstract

“…Similarly, in the study conducted by Blondin et al [8], 24% versus 39% of embryos with ≥16 cells were obtained using oocytes from 2.7-8 mm and ≥8 mm follicles, respectively, on post-insemination day 5, while oocytes from follicles ≤2.7 mm failed to reach this stage. Other investigators [9] yielded significantly more blastocysts using oocytes from 11-15 mm follicles than with those from 6-10 and 2-5 mm follicles when combining oocytes collected during the growth and static phases of follicular development. Collectively, the available results indicate that in adult cattle, oocytes from large follicles are more developmentally competent in vitro than oocytes from follicles of medium or small size.…”

mentioning

confidence: 99%

The <i>In Vitro</i> Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter

Kauffold¹,

Amer

Bergfeld³

et al. 2005

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. This study investigated the relationship between follicle size (FS) and developmental competence of calf oocytes. Cumulus-oocyte-complexes (COCs) from follicles >8 (L-COCs; n=19), 4-8 (M-COCs; n=54), and 2-3 mm (S-COCs; n=155) were recovered from non-stimulated 1-4 months old dairy calves post mortem and ex vivo (laparoscopy), and in parallel from slaughtered adult cows from follicles of identical size categories [> 8 (n=91); 4-8 (n=138); 2-3 mm (n=193)]. Morphologically intact COCs were subjected to in vitro maturation, fertilization, and embryo culture. Cleavage rate (CR; 46 h post-insemination=p.i.), rate of morulae/blastocysts (M/Bl; day 7 p.i.), and blastocysts (Bl; day 9 p.i.) were recorded. FS had no effect on the CR in calves. However, calf L-COCs yielded the highest rates of M/Bl and Bl compared with the two other size categories (P<0.05). In contrast, calf S-and M-COCs gave similar rates of M/Bl, whereas the proportion of Bl was lowest for S-COCs (P<0.05). This was almost identical to findings in cows, except that the CR was highest for L-COCs and M/Bl yields were lowest for S-COCs (P<0.05). There were no differences between calf and cows with regard to CR for the respective FS categories. L-COCs from calves and cows yielded similar rates of M/Bl and Bl, whereas calf S-and M-COCs yielded lower rates of Bl than S-and M-COCs from cows and a lower rate of M/Bl when S-and M-COCs were analyzed as one group (P<0.05). Whereas the CR was similar in calves and cows, calf COCs yielded lower rates of M/Bl and Bl (P<0.05). In conclusion, the results show that the developmental competence of calf oocytes is higher in those derived from follicles larger than 8 mm, and thus are almost equally as competent as cow oocytes derived from follicles of identical size. This suggests that calf oocytes acquire developmental competence within the large follicle, potentially due to a process similar to prematuration of the oocyte in the adult cow. It is proposed that procedures that facilitate prematuration, such as "coasting" following a preceding superstimulation, might increase the developmental competence of calf oocytes.

show abstract

Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production

Cited by 104 publications

References 24 publications

In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity

The <i>In Vitro</i> Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter

Contact Info

Product

Resources

About