Developmental changes of steryl esters in normal and jimpy mouse brain¹

Myelin

“…The myelin-associated sialosylgalactosykeramide (GM4) has not yet been sought in mutant brain. The cholesterol content of brain is reduced by 40-50% after 18 days Sheads et al, 1974). Cholesterol esters amount to less than 0.4% of total sterol in mouse brain, while the jimpy mutant demonstrates a progressive and more rapid increase in cholesterol esters after 12 days with a peak value of 1.1 % of total sterol.…”

Comparison of lipids in total brain tissue from five mouse genotypes

“…As a result of our initial work with whole brain (Campagnoni, Campagnoni, Huang and Sampugna, 1972;Lerner, Campagnoni and Sampugna, 1974;Sheads, Sampugna and Campagnoni, 1974) and particularly with subcellular fractions (unpublished observations), we recognize that future studies involving many lipid constituents may require the use of both male and female animals. The pooling of animals would be particularly advantageous in studies utilizing the "quaking" mutants, where both females and males are afflicted with a genetically determined myelin abnormality.…”

Myelin

“…The myelin-associated sialosylgalactosylceramide ({ry-ganglioside) has not yet been examined in mutant brain. The cholesterol content of brain is reduced by 40-50% after 18 days Sheads et al, 1974). Cholesterol esters amount to less than 0.4% of total sterol in mouse brain, while the jimpy mutant demonstrates a progressive and more rapid increase in cholesterol esters after 12 days with a peak value of 1.1% of total sterol.…”