Brain tissue from adult male and female mice of the C57BL/6J, C57BL/6J-AW-J, BALB/cJ, SJL/J, and DBA/2J genotypes was examined for brain weight, total protein, total lipid, cholesterol, phospholipid, plasmalogen, sulfatide, nonganglioside-glycolipid sphingosine, and ganglioside N-acetyl neuraminic acid, fatty acid, and sphingosine. No significant differences were found between sexes for any of these constituents. When compared to the overall average obtained for other animals, the DBA/2J, C57BL/6J-AW-J, and BALB/cJ mice contained lower quantities of plasmalogen and sulfatide compared to the overall averages obtained for the other genotypes. In addition, the sterol content in DBA/2J mice was significantly higher than the overall average value obtained for the other animals.
Procedures for the quantitative estimation of sialic acid, sphingosine and fatty acid were employed to assess the purity of commerically available ganglioside standards. Based on the weight printed on the label, experimental values of mono‐, di‐ and trisialoganglioside standards represented approximately 7, 77 and 25%, respectively, of the expected value. Thin layer chromatography (TLC) revealed that the monosialoganglioside was contaminated with phospholipid, possibly phosphatidyl choline. Trisialoganglioside was resolved into three spots on TLC, two of which were ninhydrin positive and resorcinol negative. These results suggest that caution should be observed when dealing with commercially available standards even though a high degree of purity is guaranteed.
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