Developmental Changes in the Sterol Composition and the Glycerol Content of Cuticular and Internal Lipids of Three Species of Flies

Gołębiowski, Marek; Cerkowniak, Magdalena; Boguś, Mieczysława I.; Włóka, Emilia; Przybysz, E; Stepnowski, Piotr

doi:10.1002/cbdv.201200419

Cited by 20 publications

(19 citation statements)

References 52 publications

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“…Glycerol prevents insects from freezing . This compound has been found in the hygroscopic secretions collected from the secretory setae of the biting midge Forcipomyia nigra larvae , Eurosta solidaginis , and in the cuticular lipids of the larvae, pupae, male, and female of M. domestica , S. carnaria , and C. vicina .…”

Section: Discussionmentioning

confidence: 99%

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Effect of Conidiobolus coronatus on the Cuticular and Internal Lipid Composition of Tettigonia viridissima Males

Gołębiowski

Cerkowniak

Ostachowska

et al. 2016

Chemistry & Biodiversity

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Conidiobolus coronatus is an entomopathogenic fungus which has a potential as a biological control agent of insects. The cuticular and internal lipid composition of infected and noninfected Tettigonia viridissima males were analyzed by GC/MS. A total of 49 compounds were identified in the infected and noninfected males, including fatty acids, fatty acid methyl esters (FAMEs), n-alkanes, alcohols, sterols, and other organic compounds. The most abundant components of the cuticular and internal lipids of the insects were fatty acids. After exposure to C. coronatus, the cuticular lipids of the T. viridissima males contained 17 free fatty acids from C(8) to C(22), while the cuticular lipids of the noninfected insects contained only 15 fatty acids from C(12) to C(24). The cuticular and internal lipids of both the infected and the noninfected males also contained five FAMEs from C(15) to C(19), seven n-alkanes from C(25) to C(34), five alcohols from C(16) to C(25), five sterols, and the following six other organic compounds: azelaic acid, phenylacetic acid, glutaric acid, benzoic acid, sebacic acid, and glycerol. The compounds which were present only in the cuticular lipids of the infected males could be due to fungal infection.

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Section: Discussionmentioning

confidence: 99%

“…Insects need sterols for development . These compounds have been found in Manduca sexta , M. domestica , Heliothis zea , Tenebrio molitor , Sarcophaga carnaria , and C. vicina .…”

Section: Discussionmentioning

confidence: 99%

Effect of Conidiobolus coronatus on the Cuticular and Internal Lipid Composition of Tettigonia viridissima Males

Gołębiowski

Cerkowniak

Ostachowska

et al. 2016

Chemistry & Biodiversity

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“…Like the hydrocarbons, these lipids are known to provide resistance to desiccation, fungal infection, and bacterial infection. A number of sterols have also been reported, including cholesterol, campesterol, and sitosterol, although cholesterol appears to be the most consistently dominant [35]. …”

Section: Introductionmentioning

confidence: 99%

Factors Affecting Species Identifications of Blow Fly Pupae Based upon Chemical Profiles and Multivariate Statistics

Kranz

Carroll

Dixon

et al. 2017

Insects

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Alternative methods for the identification of species of blow fly pupae have been developed over the years that consist of the analyses of chemical profiles. However, the effect of biotic and abiotic factors that could influence the predictive manner for the tests have not been evaluated. The lipids of blowfly pupae (Cochliomyia macellaria, Lucilia cuprina, Lucilia sericata, and Phormia regina) were extracted in pentane, derivatized, and analyzed by total-vaporization solid phase microextraction gas chromatography-mass spectrometry (TV-SPME GC-MS). Peak areas for 26 compounds were analyzed. Here we evaluated one biotic factor (colonization) on four species of blow flies to determine how well a model produced from lipid profiles of colonized flies predicted the species of flies of offspring of wild-caught flies and found very good species identification following 10 generations of inbreeding. When we evaluated four abiotic factors in our fly rearing protocols (temperature, humidity, pupation substrate, and diet), we found that the ability to assign the chemical profile to the correct species was greatly reduced.

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“…These epicuticular compounds consist of primarily hydrocarbons, but free fatty acids, fatty alcohols, aldehydes, ketones, wax esters, primary and secondary alcohol esters or acylglycerols are also commonly found (Blomquist and Jackson ; Buckner ; Gołębiowski et al . , , , ,b; Gołębiowski et al .,). The presence of such variety of lipid compounds causes waterproofing of cuticle.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of untypical lipid compounds in the cuticular and internal lipids of four fly species

et al. 2013

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Aims: This article describes the qualitative and quantitative analyses of untypical compounds in the cuticular and internal lipids of four dipteran species. For isolated compounds, antimicrobial activity against 18 reference strains of bacteria and fungi was determined. Methods and Results: In this study, gas chromatography (GC) combined with mass spectrometry (GC-MS) was used to analyse the surface and internal compounds of four fly species. Seven untypical compounds from both preimaginal and imaginal stages of examined insects were identified. Azelaic acid (AA) was the most abundant, while phenylacetic and phenylpropionic acids occurred in lower concentration. Minor quantities of sebacic acid, 2-methyl-2-hydroxybutanoic acid, tocopherol acetate and trace amounts of 2,4-decadienal were also detected. Tocopherol acetate was found only in cuticular lipids of Musca domestica larvae. Each compound was tested against several species of fungi and bacteria by determining minimal inhibitory concentration (MIC). Human pathogenic fungi were also investigated. Phenylpropionic acid showed the greatest antifungal activity. Bacterial strains were insensitive to the presence of identified compounds, apart from 2,4-decadienal which strongly inhibited bacterial growth. Conclusions: This is the first time that the chemical composition and the antimicrobial activity of untypical compounds in the cuticular and internal lipids of four fly species has been analysed. Significance and Impact of the Study: Determination of untypical compounds and their antimicrobial activity can effectively contribute to the knowledge concerning insect defence mechanisms.

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Developmental Changes in the Sterol Composition and the Glycerol Content of Cuticular and Internal Lipids of Three Species of Flies

Cited by 20 publications

References 52 publications

Effect of Conidiobolus coronatus on the Cuticular and Internal Lipid Composition of Tettigonia viridissima Males

Effect of Conidiobolus coronatus on the Cuticular and Internal Lipid Composition of Tettigonia viridissima Males

Factors Affecting Species Identifications of Blow Fly Pupae Based upon Chemical Profiles and Multivariate Statistics

Antimicrobial activity of untypical lipid compounds in the cuticular and internal lipids of four fly species

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