Developmental Changes in Creatine Kinase and Aldolase Isoenzymes and their Possible Function in Association with Contractile Elements

Turner, David C.; Eppenberger, Hans M.

doi:10.1159/000481062

Cited by 12 publications

(5 citation statements)

References 29 publications

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“…Skeletal-muscle-fiber damage and the clinical picture C Ki saw i d e l yu s e dm a r k e ri nm u s c l ed i s e a s e ,a n dt h e degree of CK elevation reflects the underlying disease process. In chronic myopathies, the CK level can drop back to normal due to loss of muscle mass (Turner and Eppenberger 1973;Giesker and Bowers 1979). Similarly, we found in this study that severely affected adult Pompe patients had near-normal CK levels, whereas less affected adults had higher levels.…”

Section: Skeletal-muscle-fiber Damagementioning

confidence: 99%

Muscle fiber‐type distribution, fiber‐type‐specific damage, and the Pompe disease phenotype

Berg

Drost

Schaart

et al. 2012

J of Inher Metab Disea

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Pompe disease is a lysosomal storage disorder caused by acid α-glucosidase deficiency and characterized by progressive muscle weakness. Enzyme replacement therapy (ERT) has ameliorated patients' perspectives, but reversal of skeletal muscle pathology remains a challenge. We studied pretreatment biopsies of 22 patients with different phenotypes to investigate to what extent fiber-type distribution and fiber-type-specific damage contribute to clinical diversity. Pompe patients have the same fiber-type distribution as healthy persons, but among nonclassic patients with the same GAA mutation (c.-32-13T>G), those with early onset of symptoms tend to have more type 2 muscle fibers than those with late-onset disease. Further, it seemed that the older, more severely affected classic infantile patients and the wheelchair-bound and ventilated nonclassic patients had a greater proportion of type 2x muscle fibers. However, as in other diseases, this may be caused by physical inactivity of those patients.

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Section: Skeletal-muscle-fiber Damagementioning

confidence: 99%

Muscle fiber‐type distribution, fiber‐type‐specific damage, and the Pompe disease phenotype

Berg

Drost

Schaart

et al. 2012

J of Inher Metab Disea

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“…Creatine kinase (CK) plays a role in dynamics of high energy phosphate formation and utilization and thereby infl uences the fl ux of many reactions of energy metabolism and electron transport [ 22 ] . CK undergoes striking changes in its activity during embryonic development and diff erentiation of muscle cells [ 23 ] . Studies from our laboratory have shown that testosterone has a region specifi c modulatory eff ect on the activities of CK, myokinase, and key enzymes involved in glycogen metabolism in the skeletal muscles of rats [ 24 , 25 ] .…”

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confidence: 99%

Transient Gestational and Neonatal Hypothyroidism-induced Specific Changes in Androgen Receptor Expression in Skeletal and Cardiac Muscles of Adult Rat

et al. 2012

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The present study aims to identify the association between androgen status and metabolic activity in skeletal and cardiac muscles of adult rats with transient gestational/neonatal-onset hypothyroidism. Pregnant and lactating rats were made hypothyroid by exposing to 0.05% methimazole in drinking water; gestational exposure was from embryonic day 9-14 (group II) or 21 (group III), lactational exposure was from postnatal day 1-14 (group IV) or 29 (group V). Serum was collected for hormone assay. Androgen receptor status, Glu-4 expression, and enzyme activities were assessed in the skeletal and cardiac muscles. Serum testosterone and estradiol levels decreased in adult rats of groups II and III, whereas testosterone remained normal but estradiol increased in group IV and V, when compared to coeval control. Androgen receptor ligand binding activity increased in both muscle phenotypes with a consistent increase in the expression level of its mRNA and protein expressions except in the forelimb of adult rats with transient hypothyroidism (group II-V). Glut-4 expression remained normal in skeletal and cardiac muscle of experimental rats. Specific activity of hexokinase and lactate dehydrogenase increased in both muscle phenotypes whereas, creatine kinase activity increased in skeletal muscles alone. It is concluded that transient gestational/lactational exposure to methimazole results in hypothyroidism during prepuberal life whereas it increases AR status and glycolytic activity in skeletal and cardiac muscles even at adulthood. Thus, the present study suggests that euthyroid status during prenatal and early postnatal life is essential to have optimal AR status and metabolic activity at adulthood.

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“…This isoenzyme has been demonstrated in young embryonic muscle (2l), and the appearance of M-type CPK in these cells has been used as a marker for muscle differentiation (l8, 29). It has, therefore, been suggested that M-type CPK is a characteristic protein of differentiated skeletal muscle (29) . Because we have shown M-type specificity for our antibody and have carefully controlled for nonspecific staining, our results show that this muscle-type protein is in nonmuscle cells.…”

Section: Discussionmentioning

confidence: 99%

“…Muscle, or M-type, is characteristic of skeletal muscle (8). Brain, or Btype, has been demonstrated in brain, nonmuscle tissue, and embryonic myoblasts (1,18,29). Transition from B-type to Mtype CPK in cultured myoblasts has been shown to reflect the differentiation of the cells (4,18,29).…”

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confidence: 99%

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Association of creatine phosphokinase with the cytoskeleton of cultured mammalian cells.

Eckert¹,

Koons²,

Schantz³

et al. 1980

The Journal of Cell Biology

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. Using an antibody specific for creatine phosphokinase (CPK), we have discovered an association between that enzyme and the cytoskeleton . Immunofluorescence observations show that CPK is associated with intermediate filaments in PTK cells and BALB/3T3 cells . The CPK distribution also follows intermediate filaments when cells are treated with colchicine .Intermediate (10-nm) filaments, microtubules, and actin filaments in the cytoplasm of cells contribute to a fibrous network known as the cytoskeleton. The role of microtubules in maintaining cell shape (10) and in organizing components of the cytoplasm (3, 7, 23) has been demonstrated in many systems (see also reference 28 for review). Intermediate filaments have been receiving increasing attention recently and are considered to be an integral part of the cytoskeleton as well . The distribution of these filaments has been determined in cultured cells by immunocytochemical methods (19) as well as in cytoskeleton models prepared by extraction with Triton X-100 (2, 25). The configuration of these filaments throughout the cytoplasm of many cells suggests a structural or supporting role (9). Desmin, a structural protein in skeletal muscle (15), is found as a component of intermediate filaments in embryonic chick cells (14), suggesting a structural role for these filaments.Although the configuration of these structural elements, as well as the characterization of their proteins, is well documented, less is known about the source of energy that may be used by contractile activities requiring cytoskeletal support. In muscle, it is well known that ATP is supplied by mitochondria . In addition, creatine phosphokinase (CPK) catalyzes the generation of ATP from creatine phosphate and ADP, maintaining an equilibrium . Thus, creatine phosphate serves as a energy source, readily converted into ATP. CPK has long been known as a mitochondrial constituent (24) where it likely catalyzes the formation of creatine phosphate from ATP and creatine . Much of the CPK is soluble, and recent evidence has shown that CPK may be found in the M-line of striated muscle (31, 33) as well as in mitochondria (24) . Immunological analysis has shown that antibodies specific for CPK will react with purified M-line protein, verifying the presence of CPK on the M-line (30) .

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Developmental Changes in Creatine Kinase and Aldolase Isoenzymes and their Possible Function in Association with Contractile Elements

Cited by 12 publications

References 29 publications

Muscle fiber‐type distribution, fiber‐type‐specific damage, and the Pompe disease phenotype

Muscle fiber‐type distribution, fiber‐type‐specific damage, and the Pompe disease phenotype

Transient Gestational and Neonatal Hypothyroidism-induced Specific Changes in Androgen Receptor Expression in Skeletal and Cardiac Muscles of Adult Rat

Association of creatine phosphokinase with the cytoskeleton of cultured mammalian cells.

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