Developmental cell death during <i>Xenopus</i> metamorphosis involves BID cleavage and caspase 2 and 8 activation

Pasquier, David Du; Rincheval, Vincent; Sinzelle, Ludivine; Chesneau, Albert; Ballagny, Chantal; Sachs, Laurent M.; Demeneix, Barbara; Mazabraud, A

doi:10.1002/dvdy.20874

Cited by 13 publications

(13 citation statements)

References 64 publications

(78 reference statements)

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“…Amphibian metamorphosis is a good model to understand the mechanisms regulating PCD [52][53][54]. The tail regression implicates an apoptotic pathway inducible by T3 hormone and involving some cell death executioners [55]. At the onset of metamorphosis, numerous apoptotic cells were observed in the spinal cord and caudal spinal ganglia [43,47,56], and a caspase-3 activity increased during climax in the spinal cord [48].…”

Section: Several Aspects Of Apoptosis At Metamorphosismentioning

confidence: 99%

Apoptosis in amphibian development

Exbrayat¹,

Moudilou²,

Abrouk³

et al. 2012

ABB

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Amphibians and more particularly X. laevis are models often used for studying apoptosis during embryonic development. Using several methods, searchers determined the localization of programmed cell deaths (PCD). Several experimental methods also have been used to understand the regulatory mechanisms of apoptosis, throughout development, contributing to elucidate the general action of several genes and proteins. Apoptosis occurs very early, with a first program under control of maternal genes expressed before MBT, in order to eliminate damaged cells before gastrulation, and a second program at the onset of gastrulation. PCD is also observed during neurulation. Then, apoptotic cells are observed in amphibian organogenesis and metamorphosis. Results of these researches showed both importance of PCD for embryonic development, and the complexity of its regulation. Results obtained can be useful to understand others aspects of the importance of apoptosis, particularly pathological aspects.

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Section: Several Aspects Of Apoptosis At Metamorphosismentioning

confidence: 99%

Apoptosis in amphibian development

Exbrayat¹,

Moudilou²,

Abrouk³

et al. 2012

ABB

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“…Although cold-blooded animals like zebrafish and Xenopus are appropriate models in the study of apoptotic processes (Du Pasquier et al, 2006; Eimon and Ashkenazi 2010; Jette et al, 2008), little is known about the Bcl-2 family in non-mammalian vertebrates. Hsieh et al cloned the zebrafish ortholog of Bad and demonstrated that it is involved in apoptosis.…”

Section: Resultsmentioning

confidence: 99%

“…Du Pasquier et al analyzed Bid expression in larval and adult tissues. In functional analogy to the mammalian protein, Bid was localized to the mitochondrion, inducing a drop of mitochondrial membrane potential (ΔΨm) (Du et al, 2006). …”

Section: Resultsmentioning

confidence: 99%

Identification of axolotl BH3-only proteins and expression in axolotl organs and apoptotic limb regeneration tissue

et al. 2018

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Like other urodela amphibians, axolotls are able to regenerate lost appendages, even as adults, rendering them unique among higher vertebrates. In reaction to the severe trauma of a lost limb, apoptosis seems to be primarily implicated in the removal of injured cells and tissue homeostasis. Little, however, is known about apoptotic pathways and control mechanisms. Therefore, here we provide additional information regarding the mechanisms of tissue degradation. Expression patterns of Bcl-2 family members were analyzed using reverse transcriptase-PCR, western blotting and immunofluorescence. In our study, we identified ten putative axolotl orthologs of the Bcl-2 family. We demonstrated that BH3-only proteins are differentially expressed in some axolotl organs, while they are expressed broadly in tail composite tissue and limb regeneration blastema. The importance of Bcl-2 family members is also indicated by detecting the expression of proapoptotic protein Bak in spatial congruence to apoptosis in the early stages of limb regeneration, while Bcl-2 expression was slightly modified. In conclusion, we demonstrate that Bcl-2 family members are conserved in the axolotl and might be involved in the tissue degradation processes that occur during limb regeneration.

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“…Proteins-Five members of the Xenopus Bcl-2 family of proteins (xBcl-w/xR1, xBcl-x L /xR11, xBax, xBid, and xBok) were functionally characterized in the previous studies (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). In addition, partial or complete nucleotide sequences of 10 Bcl-2 family proteins (xBcl-2, xBcl-B, xBcl-G, xBcl2L12, xBcl2L13, xMcl-1, xBak, xBim, xBmf, and xNoxa) were deposited in the Xenopus nucleotide database, but the functions of their protein products remained uncharacterized (42)(43)(44).…”

Section: Identification and Rt-pcr Analysis Of Xenopus Bcl-2 Familymentioning

confidence: 99%

“…They are classified as either anti-apoptotic or pro-apoptotic, and the functional balance between these two groups controls the mitochondria-mediated intrinsic pathway of apoptosis. Although more than 10 members of the Bcl-2 family of proteins have been identified in Xenopus laevis (simply referred to as Xenopus hereafter), their functional characterizations are not yet complete (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19).…”

mentioning

confidence: 99%

Anti-apoptotic Activity and Proteasome-mediated Degradation of Xenopus Mcl-1 Protein in Egg Extracts

Tsuchiya

Yamashita

2011

Journal of Biological Chemistry

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Xenopus egg extracts execute spontaneous apoptosis without the requirement of transcription and translation, and this intrinsic mechanism is supposed to be involved in the physiological elimination of aged eggs. Although apoptosis in this system is carried out by maternally stockpiled materials, the endogenous apoptosis regulators present in egg extracts are still poorly characterized. Here we examined the mRNA expression profiles and apoptosis-regulating functions of 13 Xenopus Bcl-2 family proteins in egg extracts. Among these, we found that endogenous Xenopus Mcl-1 (xMcl-1) physiologically inhibited apoptosis by counteracting the pro-apoptotic activity of endogenous Xenopus Bid in egg extracts. Exogenously added recombinant xMcl-1 was rapidly degraded by proteasome in egg extracts, and we identified the destabilizing region in the N terminus of xMcl-1. Our results suggest that the proteolytic decay of xMcl-1 may change the functional balance between pro-and anti-apoptotic activities of Bcl-2 family proteins, thereby regulating the timing of cytochrome c release in egg extracts.

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Developmental cell death during Xenopus metamorphosis involves BID cleavage and caspase 2 and 8 activation

Cited by 13 publications

References 64 publications

Apoptosis in amphibian development

Apoptosis in amphibian development

Identification of axolotl BH3-only proteins and expression in axolotl organs and apoptotic limb regeneration tissue

Anti-apoptotic Activity and Proteasome-mediated Degradation of Xenopus Mcl-1 Protein in Egg Extracts

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