Developmental bisphenol A exposure impairs sperm function and reproduction in zebrafish

Chen, Jiangfei; Saili, Katerine S.; Liu, Yueqin; Li, Lelin; Zhao, Yuxin; Jia, Yinhang; Bai, Chenglian; Tanguay, Robert L.; Dong, Qian; Huang, Changjiang

doi:10.1016/j.chemosphere.2016.11.089

Cited by 68 publications

(41 citation statements)

References 56 publications

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“…It is well-established in the current scientific literature that BPA has adverse effects on zebrafish development. 5,15 It is also known that BPA can have adverse effects on human health. 12 Little is known about the mechanism by which BPA disrupts endocrine function, and this research aims to shed light on that mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…14 A 2017 study found that exposure to concentrations of BPA as low as 0.001 M during development can severely impact zebrafish reproductive health, based on analysis of morphological traits post-exposure. 15 The amount of BPA in the environment varies widely among geographical regions. With some areas near water waste treatment plants (WWTP) found to range from not detectable to 1.6 M and other areas reporting maximums of 36.8 M and 45.1 M. 16,17,18 Marine and coastal water was also measured and showed that concentrations of BPA ranged from 0.00017 M to 0.00085 M, and portable tap water was found to have a maximum BPA concentration of 5.7 M. 16 BPA tends to exhibit a nonmonotonic dose-response curve-meaning that there is a nonlinear relationship between effects and doses.…”

Section: Introductionmentioning

confidence: 99%

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Transcription of nanos-1 in Zebrafish Embryos is not Affected by Bisphenol A: Evaluated Using Quantitative Real-Time PCR

Babich¹,

Roba²,

Safura³

et al. 2019

AJUR

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The presence of primordial germ cells (PGCs) is crucial for proper gonad formation in zebrafish (Danio rerio). The many aspects of PGC migration that allow these cells to reach the proper location at the gonadal ridge include receptors, ligands, germ plasm components, and internal maintenance of PGCs. Any one of these factors could be affected by endocrine-disrupting chemicals (EDCs), which have been shown to alter the directed migration of these cells during early embryonic development. Based on recent research wherein the EDC bisphenol A (BPA) inhibited normal PGC migration, we have used the same dose of BPA to determine the impact of BPA on a gene central to proper germ cell migration. Zebrafish embryos were exposed to BPA, and the levels of the target gene nanos-1 were analyzed using quantitative real-time PCR (q-PCR). The target gene nanos-1 is a critically important germplasm component that allows for survival and proper migration of PGCs. The q-PCR results showed that BPA did not affect the transcription level of nanos-1 in zebrafish embryos. KEYWORDS: Zebrafish; Zebrafish Embryos; nanos-1; Primordial Germ Cells; PGC Migration; Gonad Development; Endocrine-Disrupting Chemicals; Bisphenol A; Sex Determination

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcription of nanos-1 in Zebrafish Embryos is not Affected by Bisphenol A: Evaluated Using Quantitative Real-Time PCR

Babich¹,

Roba²,

Safura³

et al. 2019

AJUR

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show abstract

“…Prior studies have demonstrated that exposure to BPA at doses as low as 0.001 M negatively impact zebrafish reproductive health. 12 In 2017, researchers found that higher levels of BPA exposure in zebrafish can cause a reduction in egg production and reduce chances of fertilization. 12 Because BPA exhibits a non-monotonic dose-response curve, trace BPA concentrations in aquatic environments can still have adverse effects on aquatic organisms.…”

Section: Effects Of Endocrine-disrupting Chemicals (Edcs) On Organismsmentioning

confidence: 99%

Evaluating the Effects of Bisphenols F and S with Respect to Bisphenol A on Primordial Germ Cell Migration in Zebrafish (Danio rerio) Embryos Using Immunofluorescence Microscopy

Safura¹,

Roba²,

Freeman³

2019

AJUR

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Primordial Germ Cell (PGC) migration occurs in early embryonic development and is highly conserved across taxa. PGC migration occurs within the first 24 hours post fertilization (hpf) in zebrafish, making the organism an efficient model for observing the migration pathway. Proper PGC migration is necessary for normal gonad development and, in some species, sex determination. Disruption of this process leads to defects in gonad formation and abnormal sex determination and differentiation. Studies show that endocrine-disrupting chemicals such as bisphenol A (BPA) disrupt PGC migration in zebrafish. BPA is an estrogenic compound that has been linked to a variety of human diseases, including various cancers, diabetes, reproductive disorders, obesity, and cardiovascular diseases. It is one of the most widely used synthetic compounds worldwide, as it used to make polycarbonate plastics. Many studies provide evidence of the harmful effects of BPA on living organisms. In response, manufacturers have started to use replacements such as bisphenol F (BPF) and bisphenol S (BPS). However, due to their structural similarity, it is likely that BPF and BPS are just as harmful to organisms as BPA. In this study, we use antibody staining and immunofluorescence microscopy to confirm that BPA exposure results in abnormal PGC migration in zebrafish embryos, as previously studied, and to illustrate that BPF and BPS exposure results in similar PGC migration defects. KEYWORDS: Zebrafish; Zebrafish Embryos; Primordial Germ Cells; PGC Migration; Gonad Development; Endocrine-Disrupting Chemicals; Bisphenol A; Bisphenol S; Bisphenol F; Sex Determination

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“…Most endocrine disturbance research has focused on the impacts of estrogens, which can have a feminising effect. In human, conceptive clutter and decreased sperm numbers have been ascribed to estrogens [7,8]. Estrogenic impacts in fish, both in the research centreand in the field, have significantly been reported, including affectation of the female yolk protein in guys (vitellogenesis: e.g.…”

Section: Introductionmentioning

confidence: 99%

Dunaliella salina extract alleviates the toxic impact of Dioxin induced endocrine disruption in Nile tilapia

et al. 2019

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OLLUTION of lakes and seas in Egypt become a severe problem as it produces a lot of wastes and toxic chemicals. The water pollution induces a lot of endocrine disruptions in aquatic organisms. Several endocrine organs are influencing by pollution such as testes, ovaries, pituitary, hypothalamus, thyroid, and adrenal organs. Propositions of upsetting endocrine chemicals (EDCs) can meddle with fish larval advancement affecting the delicate balance of the biological ecosystem. Endocrine disruption effects on reproductive cycles may affect reproductive behaviour and the development of secondary sexual characteristics. So the main objective of this research is to use marine algae, Dunaliella salina, extract in fish immunity enhancement against pollutants. Nile tilapia fish were exposed to Dioxin (as the main pollutant in the Egyptian environment) after feeding on algae extracts for one month. The results revealed that Dunaliella salina was able to protect the fish against pollution as it enhances the immune capability of Nile tilapia fish due to increase the levels of the antioxidant enzymes and decrease the protein oxidation. Additionally, Dunaliella salina extract was able to decrease the alteration in the expression of genes encoding endocrine hormones such as LH and FSH in the pituitary of Nile tilapia exposed to Dioxin in comparison to control fish. Moreover, the Dunaliella salina extract decreased significantly the DNA damage in brain tissues of Nile tilapia exposed to Dioxin compared with control fish. The results conclude that Dunaliella salina could be used as ameliorative compounds against toxicity of Dioxin.

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Developmental bisphenol A exposure impairs sperm function and reproduction in zebrafish

Cited by 68 publications

References 56 publications

Transcription of nanos-1 in Zebrafish Embryos is not Affected by Bisphenol A: Evaluated Using Quantitative Real-Time PCR

Transcription of nanos-1 in Zebrafish Embryos is not Affected by Bisphenol A: Evaluated Using Quantitative Real-Time PCR

Evaluating the Effects of Bisphenols F and S with Respect to Bisphenol A on Primordial Germ Cell Migration in Zebrafish (Danio rerio) Embryos Using Immunofluorescence Microscopy

Dunaliella salina extract alleviates the toxic impact of Dioxin induced endocrine disruption in Nile tilapia

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